Study on Gene Differential Expression in Tetraploid Populus Leaves
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Growth Conditions
2.2. Measurement of Leaf Areas
2.3. Determination of the Chlorophyll Content
2.4. Determination of the Auxin Content
2.5. RNA Extraction, RNA-seq Library Preparation, and Sequencing
2.6. Quantitative Real-Time PCR Analysis of Gene Expression
2.7. Statistical Analysis
3. Results
3.1. Variation of the Polyploidy Plant Growth, Leaf Morphology and Physiological Characteristic in Poplar
3.2. Gene Expressions and Differentially Expressed Genes (DEGs) among Tetraploid, Triploid, and Diploid
3.3. Analysis of the Differential Expression and Functional Enrichment of Leaf Genes in Poplar Tetraploid and Diploid
3.3.1. GO Enrichment Analysis of Differential Genes in Poplar Tetraploid and Diploid Leaves
3.3.2. KEGG Enrichment Analysis of Differential Genes in Poplar Tetraploid and Diploid Leaves
3.4. Analysis of the Differential Expression and Functional Enrichment of Leaf Genes in Poplar Tetraploid and Triploid
3.4.1. GO Enrichment Analysis of Differential Genes in Poplar Tetraploid and Triploid Leaves
3.4.2. KEGG Enrichment Analysis of Differential Genes in Poplar Tetraploid and Triploid Leaves
4. Discussion
4.1. Differential Expression of Genes Related to Leaf Growth and Chlorophyll Synthesis of Tetraploid Poplar
4.2. Differential Expression of Auxin and Senescence-Related Genes in Tetraploid Poplar Leaves
4.3. Differential Expression of Genes Related to Chlorophyll Synthesis in Tetraploid Poplar Leaves
4.4. Differential Expression of Genes Related to Photosynthesis and Carbon Fixation in Tetraploid Poplar Leaves
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Zhang, Y.; Ren, Y.; Kang, X. Study on Gene Differential Expression in Tetraploid Populus Leaves. Forests 2020, 11, 1233. https://doi.org/10.3390/f11111233
Zhang Y, Ren Y, Kang X. Study on Gene Differential Expression in Tetraploid Populus Leaves. Forests. 2020; 11(11):1233. https://doi.org/10.3390/f11111233
Chicago/Turabian StyleZhang, Ying, Yongyu Ren, and Xiangyang Kang. 2020. "Study on Gene Differential Expression in Tetraploid Populus Leaves" Forests 11, no. 11: 1233. https://doi.org/10.3390/f11111233