Comparative Analysis Reveals the Metabolic Characteristics of Astringent Seeds of Chinese Fir (Cunninghamia lanceolata (Lamb) Hook) during Astringent Compounds Accumulation Stages
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples Collection, Processing, and Storage
2.2. Samples Preparation and Extraction
2.3. HPLC and ESI-Q TRAP-MS/MS Conditions
2.4. Qualitation and Quantitation of Metabolites
2.5. Identification and Verification of Different Expressive Metabolites
2.6. KEGG Enrichment Analysis
3. Results
3.1. Status of Astringent and Germinable Seeds
3.2. Samples Reliability Analysis
3.3. Selection and Classification of Different Expressive Metabolites
3.4. Expressive and Accumulative Change of Different Expressive Metabolites
3.5. KEGG Enrichment of Different Expressive Metabolites
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Metabolites Class | Amount | Percentage (%) | Amount in Different Categories 1 | |||
---|---|---|---|---|---|---|
A1 | A2 | B1 | B2 | |||
Alkaloids | 1 | 0.89 | 1 | 0 | 0 | 0 |
Amino acid derivatives | 10 | 8.93 | 1 | 8 | 1 | 0 |
Amino acids | 2 | 1.79 | 0 | 1 | 0 | 1 |
Anthocyanins | 3 | 2.68 | 0 | 0 | 0 | 3 |
Benzoic acid derivatives | 1 | 0.89 | 0 | 0 | 1 | 0 |
Catechin derivatives | 2 | 1.79 | 0 | 0 | 1 | 1 |
Coumarins | 3 | 2.68 | 1 | 0 | 1 | 1 |
Flavanone | 7 | 6.25 | 1 | 0 | 3 | 3 |
Flavone | 18 | 16.07 | 0 | 2 | 9 | 7 |
Flavone C-glycosides | 5 | 4.46 | 0 | 2 | 1 | 2 |
Flavonol | 15 | 13.39 | 0 | 1 | 10 | 4 |
Flavonolignan | 1 | 0.89 | 0 | 0 | 1 | 0 |
Hydroxycinnamoyl derivatives | 3 | 2.68 | 0 | 0 | 1 | 2 |
Indole derivatives | 1 | 0.89 | 0 | 1 | 0 | 0 |
Isoflavone | 4 | 3.57 | 0 | 0 | 3 | 1 |
Lipids_Fatty acids | 2 | 1.79 | 0 | 0 | 2 | 0 |
Lipids_Glycerolipids | 2 | 1.79 | 0 | 1 | 0 | 1 |
Lipids_Glycerophospholipids | 9 | 8.04 | 0 | 9 | 0 | 0 |
Nucleotide and its derivatives | 8 | 7.14 | 0 | 5 | 1 | 2 |
Organic acids | 2 | 1.79 | 0 | 0 | 0 | 2 |
Others | 3 | 2.68 | 1 | 1 | 1 | 0 |
Phenolamides | 1 | 0.89 | 0 | 1 | 0 | 0 |
Phytohormones | 4 | 3.57 | 2 | 1 | 1 | 0 |
Pyridine derivatives | 1 | 0.89 | 0 | 0 | 0 | 1 |
Tryptamine derivatives | 2 | 1.79 | 0 | 2 | 0 | 0 |
Vitamins | 2 | 1.79 | 1 | 1 | 0 | 0 |
Grand total | 112 | 100.00 |
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Chen, Y.; Wu, Y.; Wu, C.; Lin, S. Comparative Analysis Reveals the Metabolic Characteristics of Astringent Seeds of Chinese Fir (Cunninghamia lanceolata (Lamb) Hook) during Astringent Compounds Accumulation Stages. Forests 2020, 11, 1206. https://doi.org/10.3390/f11111206
Chen Y, Wu Y, Wu C, Lin S. Comparative Analysis Reveals the Metabolic Characteristics of Astringent Seeds of Chinese Fir (Cunninghamia lanceolata (Lamb) Hook) during Astringent Compounds Accumulation Stages. Forests. 2020; 11(11):1206. https://doi.org/10.3390/f11111206
Chicago/Turabian StyleChen, Yu, Yihan Wu, Chao Wu, and Sizu Lin. 2020. "Comparative Analysis Reveals the Metabolic Characteristics of Astringent Seeds of Chinese Fir (Cunninghamia lanceolata (Lamb) Hook) during Astringent Compounds Accumulation Stages" Forests 11, no. 11: 1206. https://doi.org/10.3390/f11111206