Next Article in Journal
On the Management of Large-Diameter Trees in China’s Forests
Previous Article in Journal
Impact of Land Use/Cover Change on Ecosystem Service Values in the Kilombero Valley Floodplain, Southeastern Tanzania
Open AccessArticle

Genetic Structure of Norway Spruce Ecotypes Studied by SSR Markers

Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 21 Prague, Czech Republic
*
Author to whom correspondence should be addressed.
Forests 2020, 11(1), 110; https://doi.org/10.3390/f11010110
Received: 6 December 2019 / Revised: 7 January 2020 / Accepted: 13 January 2020 / Published: 16 January 2020
(This article belongs to the Section Forest Ecophysiology and Biology)
Norway spruce is a widespread and economically highly important tree species in Central Europe which occurs there in different morphotypic forms (also known as ecotypes). Previously established common garden experiments indicated that the morphological differentiation is most likely genetically determined. The genetic structure of Norway spruce morphological variants might be an indicator (marker) of specific sustainability in forest ecosystems. In this study, we investigated 436 individuals from autochthonous populations belonging to three different ecotypes. The main aim was to evaluate a level of genetic intra and interpopulation diversity among the low, medium and high-elevation ecotypes using both expressed sequence tag simple sequence repeats (EST – SSR) and genomic SSR markers. Sixteen highly polymorphic microsatellite loci folded in two newly designed multiplexes were used to depicture the genetic structure of targeted trees. Important allele frequency parameters, such as the mean expected (0.722, SE = 0.061) and observed (0.585, SE = 0.062) heterozygosity and mean effective number of alleles (Ne = 5.943, SE = 1.279), were estimated. The low genetic differentiation detected among different ecotypes (Fst = 0.008) was further discussed and clarified. View Full-Text
Keywords: Picea abies (L.) Karst; microsatellite genotyping; genetic variation; three forms Picea abies (L.) Karst; microsatellite genotyping; genetic variation; three forms
Show Figures

Graphical abstract

MDPI and ACS Style

Bínová, Z.; Korecký, J.; Dvořák, J.; Bílý, J.; Zádrapová, D.; Jansa, V.; Lstibůrek, M. Genetic Structure of Norway Spruce Ecotypes Studied by SSR Markers. Forests 2020, 11, 110.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop