Optimization of Pollen Germination in Tectona grandis (Teak) for Breeding Programs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Effect of Dehydration on the Viability and Germination of Pollen Inside and Outside the Anther
2.2. Effect of pH on Viability and in vitro Germination of Pollen
2.3. Determination of the Dehydration Curve and Survival of Pollen
2.4. Effect of the Culture Medium on the Viability and Germination of Pollen
2.5. Statistic Analysis
3. Results
3.1. Effect of Dehydration on the Viability and Germination of Pollen Analyzed Inside and Outside the Anther
3.2. Effect of pH on the Viability and In Vitro Germination of Pollen
3.3. Determination of the Dehydration Curve and Survival of Pollen
3.4. Effect of the Culture Media on the Viability and Germination of Pollen
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Medium | Formulation |
---|---|
Medium 1 | 10% sucrose (g/100 mL) in aqueous solution at a pH of 7 |
Medium 2 | 14% sucrose (g/100 mL) in aqueous solution at a pH of 7 |
Medium 3 | BK medium at 10% of its salts + 10% sucrose (g/100 mL) in aqueous solution at a pH of 7 |
Dehydration (h) | Viability (%) | Germination (%) | ||
---|---|---|---|---|
Inside | Outside | Inside | Outside | |
0 | 75.47 ± 10.29 c | 86.45 ± 1.38 a | 43.22 ± 2.18 a | 52.72 ± 2.01 a |
2 | 90.00 ± 0.00 a | 87.96 ± 0.85 a | 20.77 ± 3.71 b | 23.28 ± 1.74 bc |
4 | 89.00 ± 3.47 a | 84.83 ± 2.07 b | 4.68 ± 1.3 c | 32.73 ± 4.33 ab |
6 | 83.20 ± 13.87 b | 70.46 ± 7.24 c | 10.07 ± 1.95 c | 21.69 ± 2.57 c |
Time (h) | pH | Variability (%) | Germination (%) |
---|---|---|---|
0 | 6 | 74.72 ± 1.91 abcd | 19.61 ± 2.59 abc |
6.3 | 72.25 ± 6.01 bcd | 16.35 ± 2.36 bc | |
6.5 | 77.95 ± 4.92 abcd | 29.44 ± 3.87 abc | |
6.8 | 68.33 ± 1.62 cd | 19.48 ± 5.05 abc | |
7 | 65.54 ± 6.30 d | 29.96 ± 2.66 abc | |
2 | 6 | 73.60 ± 1.73 bcd | 32.23 ± 2.97 abc |
6.3 | 83.58 ± 2.76 abc | 36.28 ± 10.55 abc | |
6.5 | 86.11 ± 3.89 ab | 41.15 ± 7.69 ab | |
6.8 | 90.00 ± 0.00 a | 29.00 ± 8.50 abc | |
7 | 83.88 ± 3.78 abc | 45.78 ± 9.99 a | |
4 | 6 | 74.44 ± 2.12 abcd | 13.62 ± 1.56 c |
6.3 | 77.67 ± 0.83 abcd | 42.36 ± 5.29 ab | |
6.5 | 81.42 ± 0.5 abcd | 12.81 ± 1.9 c | |
6.8 | 79.74 ± 2.58 abcd | 15.57 ± 2.77 bc | |
7 | 78.14 ± 0.77 abcd | 12.81 ± 1.12 c |
Medium | Viability (%) | Germination (%) |
---|---|---|
Medium 1 | 90 | 5.14 ± 1.33 b |
Medium 2 | 90 | 5.35 ± 1.26 b |
Medium 3 | 90 | 28.99 ± 1.53 a |
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Hine, A.; Rojas, A.; Suarez, L.; Murillo, O.; Espinoza, M. Optimization of Pollen Germination in Tectona grandis (Teak) for Breeding Programs. Forests 2019, 10, 908. https://doi.org/10.3390/f10100908
Hine A, Rojas A, Suarez L, Murillo O, Espinoza M. Optimization of Pollen Germination in Tectona grandis (Teak) for Breeding Programs. Forests. 2019; 10(10):908. https://doi.org/10.3390/f10100908
Chicago/Turabian StyleHine, Ana, Alejandra Rojas, Lorenzo Suarez, Olman Murillo, and Mario Espinoza. 2019. "Optimization of Pollen Germination in Tectona grandis (Teak) for Breeding Programs" Forests 10, no. 10: 908. https://doi.org/10.3390/f10100908