Bacillus subtilis is a Potential Degrader of Pyrene and Benzo[a]pyrene
Abstract
:Introduction
Materials and Methods
Chemicals and Reagents
Preparation of Media
Isolation of Bacteria from PAH-Contaminated Soil
Growth of Tgr3 in Various Media
Tgr3 Viability in the Presence of Different PAH
Assessment of Degradation Capacity of Tgr3
Analysis of PAH Extracted from Transformation Experiment
Results
Isolation of Bacteria from PAH-Contaminated Soil
Transformation Experiments
Discussion
Isolation of Potential HMW-PAH Degrading Bacteria
Conclusions
Bacterium | Characteristics | ||
---|---|---|---|
Morphology | Gram Stain | Other Features | |
Tgr1 | Rod Shaped | − | Cloudy w/yellow flocculation |
Tgr 2 | Rod Shaped | − | Cloudy white |
Tgr 3 | Rod Shaped | + | Cloudy white |
Tgr 4 | Rod Shaped | − | Cloudy white |
Tgr 5 | Rod Shaped | − | Cloudy white |
Tgr 7 | Rod Shaped | + | Cloudy white |
Tgr 8 | Rod Shaped | + | Cloudy white |
Tgr 9 | Rod Shaped | − | Cloudy white |
Tgr 10 | Rod Shaped | + | Slightly cloudy |
Tgr 11 | Rod Shaped | − | Cloudy white |
Tgr 12 | Rod Shaped | − | Slightly cloudy |
Acknowledgements
References
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Hunter, R.D.; Ekunwe, S.I.N.; Dodor, D.E.; Hwang, H.-M.; Ekunwe, L. Bacillus subtilis is a Potential Degrader of Pyrene and Benzo[a]pyrene. Int. J. Environ. Res. Public Health 2005, 2, 267-271. https://doi.org/10.3390/ijerph2005020010
Hunter RD, Ekunwe SIN, Dodor DE, Hwang H-M, Ekunwe L. Bacillus subtilis is a Potential Degrader of Pyrene and Benzo[a]pyrene. International Journal of Environmental Research and Public Health. 2005; 2(2):267-271. https://doi.org/10.3390/ijerph2005020010
Chicago/Turabian StyleHunter, Rochelle D., Stephen I. N. Ekunwe, Daniel E. Dodor, Huey-Min Hwang, and Lynette Ekunwe. 2005. "Bacillus subtilis is a Potential Degrader of Pyrene and Benzo[a]pyrene" International Journal of Environmental Research and Public Health 2, no. 2: 267-271. https://doi.org/10.3390/ijerph2005020010