Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture
2.3. Modeling of Transdifferentiation
2.4. Small-Molecule Intervention and Treatment of NIH-3T3 Cells
2.5. Transfection of Small-Interfering RNA
2.6. Animals
2.7. Animal Model of Silicosis
2.8. RT-qPCR
2.9. Western Blot Analysis
2.10. Histology
2.11. Immunohistochemistr (IHC)
2.12. Statistical Analysis
3. Result
3.1. TGF-β1 Induces Cells Transdifferentiation in a Dose-Dependent Manner
3.2. TGF-β1 Causes Cellular Transdifferentiation in a Time-Dependent Manner
3.3. HIF-1α and PSMAD3 Reached Maximum Expression at the Same Time
3.4. HIF-1α Affects the Phosphorylation of SMAD3 and the Expression of COL1A1
3.5. Expression of α-SMA and Col1a Was Affected by Knockdown of HIF-1α Using siRNA
3.6. SIS3 Inhibits DMOG Induced Elevation of COL1A1
3.7. KC7F2 Alleviates Silica Particles Induced PF in Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Forward, 5′-3′ | Reverse, 5′-3′ |
---|---|---|
COL1A1 | TAAGGGTCCCCAATGGTGAGA | GGGTCCCTCGACTCCTACAT |
α-SMA | GGCACCACTGAACCCTAAGG | ACAATACCAGTTGTACGTCCAGA |
SMAD3 | AGACGCCAGTTCTACCTCCAGTG | GCCAGCAGGGAAGTTAGTGTTCTC |
HIF-1α | CTGCCACTGCCACCACAACTG | TGCCACTGTATGCTGATGCCTTAG |
β-Actin | GTGCTATGTTGCTCTAGACTTCG | ATGCCACAGGATTCCATACC |
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Xu, X.; Li, Y.; Niu, Z.; Xia, J.; Dai, K.; Wang, C.; Yao, W.; Guo, Y.; Deng, X.; He, J.; et al. Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis. Int. J. Environ. Res. Public Health 2022, 19, 6775. https://doi.org/10.3390/ijerph19116775
Xu X, Li Y, Niu Z, Xia J, Dai K, Wang C, Yao W, Guo Y, Deng X, He J, et al. Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis. International Journal of Environmental Research and Public Health. 2022; 19(11):6775. https://doi.org/10.3390/ijerph19116775
Chicago/Turabian StyleXu, Xiao, Yiping Li, Zhuoya Niu, Jiarui Xia, Kai Dai, Chen Wang, Wu Yao, Yonghua Guo, Xuedan Deng, Jing He, and et al. 2022. "Inhibition of HIF-1α Attenuates Silica-Induced Pulmonary Fibrosis" International Journal of Environmental Research and Public Health 19, no. 11: 6775. https://doi.org/10.3390/ijerph19116775