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Comment published on 19 January 2021, see Int. J. Environ. Res. Public Health 2021, 18(2), 793.
Article

Leptospira Detection in Cats in Spain by Serology and Molecular Techniques

1
Department de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, Barcelona (UAB), CP 08193 Bellaterra, Espana
2
Wildlife Ecology & Health group (WE&H), Servei d’Ecopatologia de Fauna Salvatge (SEFaS), Universitat Autònoma de Barcelona (UAB), CP 08193 Bellaterra, Espana
3
OIE and National Collaborating Centre for Reference and Research on Leptospirosis (NRL), Amsterdam UMC, University of Amsterdam, Medical Microbiology, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands
4
Clínica Felina Barcelona, CP 08015 Barcelona, Espana
5
Facultad de Veterinaria, Universidad de Extremadura, CP 10003 Cáceres, Espana
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2020, 17(5), 1600; https://doi.org/10.3390/ijerph17051600
Received: 7 February 2020 / Revised: 26 February 2020 / Accepted: 27 February 2020 / Published: 2 March 2020
(This article belongs to the Section Infectious Disease Epidemiology)
Leptospirosis is the most neglected widespread zoonosis worldwide. In Spain, leptospirosis reports in people and animals have increased lately. Cats can become infected with Leptospira, as well as be chronic carriers. The aim of this study was to determine serological antibody prevalence against Leptospira sp., blood DNA, and shedding of DNA from pathogenic Leptospira species in the urine of cats in Spain. Microagglutination tests (MAT) and blood and urine TaqMan real-time polymerase chain reaction (PCR) were performed. Leptospira antibodies were detected in 10/244 cats; with 4.1% positive results (95% confidence interval (CI): 2.1–7.18%). Titers ranged from 1:20 to 1:320 (serovars Ballum; Bataviae; Bratislava; Cynopteri; Grippotyphosa Mandemakers; Grippotyphosa Moskva; Pomona; and Proechimys). The most common serovar was Cynopteri. Blood samples from 1/89 cats amplified for Leptospira DNA (1.12%; 95% CI: 0.05–5.41%). Urine samples from 4/232 cats amplified for Leptospira DNA (1.72%; 95% CI: 0.55–4.10%). In conclusion free-roaming cats in Spain can shed pathogenic Leptospira DNA in their urine and may be a source of human infection. Serovars not previously described in cats in Spain were detected; suggesting the presence of at least 4 different species of pathogenic leptospires in the country (L. borgpetersenii; L. interrogans; L. kirschneri; and L. noguchii). View Full-Text
Keywords: antibodies; free-roaming cat; leptospirosis; PCR; shedding; serovar; zoonoses antibodies; free-roaming cat; leptospirosis; PCR; shedding; serovar; zoonoses
MDPI and ACS Style

Murillo, A.; Cuenca, R.; Serrano, E.; Marga, G.; Ahmed, A.; Cervantes, S.; Caparrós, C.; Vieitez, V.; Ladina, A.; Pastor, J. Leptospira Detection in Cats in Spain by Serology and Molecular Techniques. Int. J. Environ. Res. Public Health 2020, 17, 1600. https://doi.org/10.3390/ijerph17051600

AMA Style

Murillo A, Cuenca R, Serrano E, Marga G, Ahmed A, Cervantes S, Caparrós C, Vieitez V, Ladina A, Pastor J. Leptospira Detection in Cats in Spain by Serology and Molecular Techniques. International Journal of Environmental Research and Public Health. 2020; 17(5):1600. https://doi.org/10.3390/ijerph17051600

Chicago/Turabian Style

Murillo, Andrea, Rafaela Cuenca, Emmanuel Serrano, Goris Marga, Ahmed Ahmed, Salvador Cervantes, Cristina Caparrós, Verónica Vieitez, Andrea Ladina, and Josep Pastor. 2020. "Leptospira Detection in Cats in Spain by Serology and Molecular Techniques" International Journal of Environmental Research and Public Health 17, no. 5: 1600. https://doi.org/10.3390/ijerph17051600

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