A qPCR-Based Tool to Diagnose the Presence of Harmful Cyanobacteria and Cyanotoxins in Drinking Water Sources
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Description: Figure S1 – Locations of the studied reservoirs: (a) 10 studied reservoirs in Taiwan, (b) 9 studied reservoirs in Matsu, and (c) 10 studied reservoirs in Kinmen. The Hsin-Shan Reservoir (HSR), Shih-Men Reservoir (SMR), Bao-Shan Reservoir (BSR), Bao-Shan Second Reservoir (BSSR), Liyutan Reservoir (LYTR), Lan-Tan Reservoir (LTR), Nan-Hua Reservoir (NHR), Agongdian Reservoir (AGDR), and Fong-Shan Reservoir (FSR) in Taiwan; the Hou-Wo Reservoir (HWR), Chu-Shui-Wo Lower Dam (CSWLD), Chu-Shui-Wo Upper Dam (CSWUD), Jin-Sha Reservoir (JSR-M), First Jin-Sha Reservoir (FJSR), Sheng-Li Reservoir (SLR), Tsair-Pu-Wo Reservoir (TPWR), Le-Dao-Wo Reservoir (LDWR), and Jhu-Luo Reservoir (JLR) in Matsu; the Rong-Hu Reservoir (RHR), Jin-Sha Reservoir (JSR-K), Tian-Pu Reservoir (TPR), Lan-Hu Reservoir (LHR), Lian-Hu Reservoir (LianHR), Ling-Hu Reservoir (LingHR), Yang-Ming-Hu Reservoir (YMHR), Xi-Hu Reservoir (XHR), Jin-Hu Reservoir (JHR), and Tai-Hu Reservoir (THR) in Kinmen. Figure S2 – Tests of inhibition on gene detection caused by different amounts of standard DNA using gel electrophoresis, where M represents the DNA marker, N represents the negative control, P5R5, P3R5 and P6R3 represent the concentration of pks gene and rpoC1 gene with 2 replicates, respectively (P5R5 = 105 and 105; P3R5 = 103 and 105; P6R3 = 106 and 103). (a) is for the duplex qPCR system with primer and probe sets of pks gene and rpoC1 gene; (b) is for the duplex qPCR system with primer sets of pks gene and rpoC1 gene (without probes). Figure S3 – The relationship between cell enumeration measured with microscopy and gene copy number with qPCR, where (a) is for 16S rRNA gene, (b) is for mcyB gene, and (c) is for rpoC1 gene. Error bars represent standard deviation of 2 replicates. Table S1 – Detailed information of oligonucleotides. Table S2 – Monitoring results of Microcystis and microcystins for the samples collected from Tai-Hu Reservoir (THR). Table S3 – Monitoring results of Cylindrospermopsis and cylindrospermopsin for the samples collected from Tai-Hu Reservoir (THR). Table S4 –The influence of primer concentration on the inhibition of gene detection. Table S5 – Correlation between MCs/CYN concentrations and cell equivalents.
Chiu, Y.-T.; Chen, Y.-H.; Wang, T.-S.; Yen, H.-K.; Lin, T.-F. A qPCR-Based Tool to Diagnose the Presence of Harmful Cyanobacteria and Cyanotoxins in Drinking Water Sources. Int. J. Environ. Res. Public Health 2017, 14, 547.
Chiu Y-T, Chen Y-H, Wang T-S, Yen H-K, Lin T-F. A qPCR-Based Tool to Diagnose the Presence of Harmful Cyanobacteria and Cyanotoxins in Drinking Water Sources. International Journal of Environmental Research and Public Health. 2017; 14(5):547.Chicago/Turabian Style
Chiu, Yi-Ting; Chen, Yi-Hsuan; Wang, Ting-Shaun; Yen, Hung-Kai; Lin, Tsair-Fuh. 2017. "A qPCR-Based Tool to Diagnose the Presence of Harmful Cyanobacteria and Cyanotoxins in Drinking Water Sources." Int. J. Environ. Res. Public Health 14, no. 5: 547.