Benzene Exposure Alters Expression of Enzymes Involved in Fatty Acid β-Oxidation in Male C3H/He Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Antibodies
2.2. Animals and Treatment
2.3. Blood Routine
2.4. L-Carnitine Assay
2.5. Real-Time Polymerase Chain Reaction (PCR)
2.6. Western Blot
2.7. ATP Assay
2.8. Mitochondrial Membrane Potential Measurement
2.9. Measurement of Reactive Oxygen Species (ROS) Level
2.10. Measurement of H2O2 Level
2.11. Measurement of Malondialdehyde (MDA) Level
2.12. Statistical Analysis
3. Results
3.1. Effects of Benzene on Body Weight
3.2. Benzene Causes Declines in Mouse WBC, RBC, Pit Count, and Hgb Concentration
3.3. Benzene Induces Reduced L-Carnitine and Alters Expression of Key Genes in the FAO Pathway in Mouse BM Cells
3.4. Benzene Induces Increased Expression of Key Fatty Acid (FA) Transport Proteins in Mouse BM Cells
3.5. Benzene Induces Increased Expression of Key FAO Proteins in Mouse BM Cells
3.6. Benzene Induces Mitochondrial Dysfunction
3.7. Benzene Induces Oxidative Stress and Lipid Peroxidation
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Primer Name | Primer Sequence (5′-3′) |
---|---|
Actin | Forward: CGAGCGGTTCCGATGCCCTG; Reverse: ACGCAGCTCAGTAACAGTCCGC |
Cpt1a | Forward: CGATCATCATGACTATGCGCTACT; Reverse: GCCGTGCTCTGCAAACATC |
Crat | Forward: GCTTGTGCGGTCTCCTATGGT; Reverse: TTGGCTTTCTCTATGTCGTTCTTG |
Hadha | Forward: AAGGGGATGTGGCAGTTATT; Reverse: ACTCCTGATTTGGTCGTTGG |
Acaa2 | Forward: CAATGAGGAGATGGCACCC; Reverse: CTTTCTTGAACACGGACGG |
Acadvl | Forward: GTGGCTCTGCAAGGCTGTA; Reverse: CGATTCCTGTCCTCCGTCTC |
Echs1 | Forward: AAGCAGGCAGGTCTTGTAAGC; Reverse: CGGTGGCAAAGGTGGAATA |
Aldh1l2 | Forward: ACCAGCCGGGTTTATTTCAAA; Reverse: ACTCCCACTACTCGGTGGC |
Crot | Forward: GGTGGCTCAATGTTGCCTAC; Reverse: TCCTCTTTCTAACTGAGTGCCT |
Group | WBC (109/L) | RBC (1012/L) | Hgb (g/L) | Pit (109/L) |
---|---|---|---|---|
Control | 7.36 ± 1.45 | 8.04 ± 0.18 | 127.75 ± 11.32 | 593.38 ± 87.43 |
Benzene | 3.08 ± 0.80 *** | 6.23 ± 0.33 * | 109.50 ± 5.50 * | 368.38 ± 82.63 * |
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Sun, R.; Cao, M.; Zhang, J.; Yang, W.; Wei, H.; Meng, X.; Yin, L.; Pu, Y. Benzene Exposure Alters Expression of Enzymes Involved in Fatty Acid β-Oxidation in Male C3H/He Mice. Int. J. Environ. Res. Public Health 2016, 13, 1068. https://doi.org/10.3390/ijerph13111068
Sun R, Cao M, Zhang J, Yang W, Wei H, Meng X, Yin L, Pu Y. Benzene Exposure Alters Expression of Enzymes Involved in Fatty Acid β-Oxidation in Male C3H/He Mice. International Journal of Environmental Research and Public Health. 2016; 13(11):1068. https://doi.org/10.3390/ijerph13111068
Chicago/Turabian StyleSun, Rongli, Meng Cao, Juan Zhang, Wenwen Yang, Haiyan Wei, Xing Meng, Lihong Yin, and Yuepu Pu. 2016. "Benzene Exposure Alters Expression of Enzymes Involved in Fatty Acid β-Oxidation in Male C3H/He Mice" International Journal of Environmental Research and Public Health 13, no. 11: 1068. https://doi.org/10.3390/ijerph13111068