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Open AccessArticle

Production of the Allergenic Protein Alt a 1 by Alternaria Isolates from Working Environments

1
Institute of Fermentation Technology and Microbiology, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland
2
Institute of General Food Chemistry, Lodz University of Technology, 171/173 Wólczańska Str, 90-924 Łódź, Poland
3
Institute of Plant Genetics, Polish Academy of Sciences, 34 Strzeszyńska Str, 60-479 Poznań, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Paul B. Tchounwou
Int. J. Environ. Res. Public Health 2015, 12(2), 2164-2183; https://doi.org/10.3390/ijerph120202164
Received: 12 January 2015 / Revised: 27 January 2015 / Accepted: 6 February 2015 / Published: 16 February 2015
The aim of the study was to evaluate the ability of Alternaria isolates from workplaces to produce Alt a 1 allergenic protein, and to analyze whether technical materials (cellulose, compost, leather) present within the working environment stimulate or inhibit Alt a 1 production (ELISA test). Studies included identification of the isolated molds by nucleotide sequences analyzing of the ITS1/ITS2 regions, actin, calmodulin and Alt a 1 genes. It has been shown that Alternaria molds are significant part of microbiocenosis in the archive, museum, library, composting plant and tannery (14%–16% frequency in the air). The presence of the gene encoding the Alt a 1 protein has been detected for the strains: Alternaria alternata, A. lini, A. limoniasperae A. nobilis and A. tenuissima. Environmental strains produced Alt a 1 at higher concentrations (1.103–6.528 ng/mL) than a ATCC strain (0.551–0.975 ng/mL). It has been shown that the homogenization of the mycelium and the use of ultrafiltration allow a considerable increase of Alt a 1 concentration. Variations in the production of Alt a 1 protein, depend on the strain and extraction methods. These studies revealed no impact of the technical material from the workplaces on the production of Alt a 1 protein. View Full-Text
Keywords: Alternaria; workplaces; Alt a 1 protein; Alt a 1 gene; ELISA Alternaria; workplaces; Alt a 1 protein; Alt a 1 gene; ELISA
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Skóra, J.; Otlewska, A.; Gutarowska, B.; Leszczyńska, J.; Majak, I.; Stępień, Ł. Production of the Allergenic Protein Alt a 1 by Alternaria Isolates from Working Environments. Int. J. Environ. Res. Public Health 2015, 12, 2164-2183.

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