Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation
Abstract
:1. Introduction
2. Experimental Section
2.1. Cell Culture and Irradiation
2.2. The Cytokinesis-Block Micronucleus Assay
2.3. Fluorescent in Situ Hybridization (FISH) Using Pan-Centromeric Probe
2.4. FISH Using Centromeric Probes of Chromosomes 1 and 4
2.5. Microscopic Examination
2.6. Statistical Analysis
3. Results
3.1. Radiation Increased the Frequency of MN, but not Delayed MN Induction
Time after Irradiation (h) | Dose (cGy) | No. of MNCB a Cells/1000 BN b Cells | Multi MNCB c/MNCB Cells (%) † | Total No. of MN d/1000 BN Cells |
---|---|---|---|---|
28 (Direct) | Control | 28.0 ± 1.4 | 9.8 ± 2.5 | 30.5 ± 2.1 |
20 | 35.0 ± 0.0 | 6.1 ± 2.2 | 37.0 ± 2.8 | |
50 | 55.5 ± 0.7 | 19.1 ± 3.4 | 65.0 ± 2.1 | |
100 | 95.5 ± 3.5 | 18.9 ± 1.4 | 113.0 ± 7.0 * | |
88 (1 passage) | Control | 30.0 ± 0.0 | 5.3 ± 1.6 | 31.5 ± 0.7 |
20 | 33.0 ± 0.0 | 6.5 ± 1.7 | 35.5 ± 2.1 | |
50 | 57.0 ± 2.8 | 9.6 ± 1.4 | 62.0 ± 5.6 | |
100 | 99.5 ± 0.7 | 13.8 ± 3.5 | 114.5 ± 3.5 * | |
240 (5 passages) | Control | 31.0 ± 2.8 | 5.1 ± 1.9 | 32.5 ± 2.1 |
20 | 33.5 ± 2.1 | 9.8 ± 2.8 | 37.0 ± 2.8 | |
50 | 58.0 ± 4.2 | 9.4 ± 1.5 | 64.0 ± 8.4 | |
100 | 101.5 ± 0.7 | 8.0 ± 4.2 | 111.0 ± 4.2 * |
3.2. Induction of MNC+ and MNC− Did Not Differ with Number of Cell Passages After X-Irradiation
3.3. Radiation Induced Delayed Aneuploidy of Chromosomes 1 and 4 in Fibroblasts
Time after Irradiation (h) | Dose (cGy) | No. of BN a Cells Scored | Normal Cells b | Aneuploid Cells | Total Aneuploidy | |
---|---|---|---|---|---|---|
Non-Disjunction c | Chromosome Loss d | |||||
28 (Direct) | Control | 1000 | 997.0 ± 0.0 | 2.5 ± 0.7 | 0.5 ± 0.7 | 3.0 ± 0.0 |
20 | 1000 | 996.5 ± 0.7 | 3.0 ± 1.4 | 0.5 ± 0.7 | 3.5 ± 0.7 | |
50 | 1000 | 993.5 ± 2.1 | 5.0 ± 1.4 | 1.5 ± 0.7 | 6.5 ± 2.1 | |
100 | 1000 | 990.0 ± 4.2 | 7.5 ± 0.7 | 2.5 ± 2.1 | 10.0 ± 2.8 * | |
88 (1 passage) | Control | 1000 | 997.0 ± 1.4 | 3.0 ± 1.4 | 0.0 ± 0.0 | 3.0 ± 1.4 |
20 | 1000 | 996.0 ± 0.0 | 3.5 ± 0.7 | 0.5 ± 0.7 | 4.0 ± 0.0 | |
50 | 1000 | 995.0 ± 1.4 | 4.0 ± 1.4 | 1.5 ± 0.7 | 5.0 ± 1.4 | |
100 | 1000 | 991.5 ± 2.1 | 7.0 ± 2.8 | 1.5 ± 0.7 | 8.5 ± 2.1 * | |
240 (5 passages) | Control | 1000 | 997.0 ± 0.0 | 2.5 ± 0.7 | 0.5 ± 0.7 | 3.0 ± 0.0 |
20 | 1000 | 992.5 ± 0.7 | 6.0 ± 0.0 | 1.5 ± 0.7 | 7.5 ± 0.7 | |
50 | 1000 | 987.0 ± 1.4 | 11.0 ± 0.0 | 2.0 ± 1.4 | 13.0 ± 1.4 | |
100 | 1000 | 979.5 ± 2.1 | 15.0 ± 0.0 | 5.5 ± 2.1 | 21.0 ± 2.1 *† |
Time after Irradiation (h) | Dose (cGy) | No. of BN a Cells Scored | Normal Cells b | Aneuploid Cells | Total Aneuploidy | |
---|---|---|---|---|---|---|
Non-Disjunction c | Chromosome Loss d | |||||
28 (Direct) | Control | 1000 | 997.5 ± 0.7 | 2.5 ± 0.7 | 0.0 ± 0.0 | 2.5 ± 0.7 |
20 | 1000 | 997.0 ± 1.4 | 2.5 ± 0.7 | 0.5 ± 0.7 | 3.0 ± 1.4 | |
50 | 1000 | 995.5 ± 2.1 | 3.5 ± 0.7 | 1.0 ± 1.4 | 4.5 ± 2.1 | |
100 | 1000 | 993.5 ± 2.1 | 5.0 ± 1.4 | 1.5 ± 0.7 | 6.5 ± 2.1 * | |
88 (1 passage) | Control | 1000 | 997.0 ± 1.4 | 2.5 ± 0.7 | 0.5 ± 0.7 | 3.0 ± 1.4 |
20 | 1000 | 998.0 ± 1.4 | 2.0 ± 1.4 | 0.0 ± 0.0 | 2.0 ± 1.4 | |
50 | 1000 | 996.0 ± 1.4 | 4.0 ± 1.4 | 0.0 ± 0.0 | 4.0 ± 1.4 | |
100 | 1000 | 993.0 ± 1.4 | 5.0 ± 1.4 | 2.0 ± 0.0 | 7.0 ± 1.4 * | |
240 (5 passages) | Control | 1000 | 997.0 ± 0.0 | 3.0 ± 0.0 | 0.0 ± 00 | 3.0 ± 0.0 |
20 | 1000 | 995.5 ± 0.7 | 3.0 ± 1.4 | 1.5 ± 0.7 | 4.5 ± 0.7 | |
50 | 1000 | 991.5 ± 0.7 | 6.5 ± 0.7 | 2.0 ± 0.0 | 8.5 ± 0.7 | |
100 | 1000 | 988.0 ± 1.4 | 8.5 ± 1.4 | 3.5 ± 0.7 | 12.0 ± 1.4 * |
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Cho, Y.H.; Kim, S.Y.; Woo, H.D.; Kim, Y.J.; Ha, S.W.; Chung, H.W. Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation. Int. J. Environ. Res. Public Health 2015, 12, 15162-15172. https://doi.org/10.3390/ijerph121214979
Cho YH, Kim SY, Woo HD, Kim YJ, Ha SW, Chung HW. Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation. International Journal of Environmental Research and Public Health. 2015; 12(12):15162-15172. https://doi.org/10.3390/ijerph121214979
Chicago/Turabian StyleCho, Yoon Hee, Su Young Kim, Hae Dong Woo, Yang Jee Kim, Sung Whan Ha, and Hai Won Chung. 2015. "Delayed Numerical Chromosome Aberrations in Human Fibroblasts by Low Dose of Radiation" International Journal of Environmental Research and Public Health 12, no. 12: 15162-15172. https://doi.org/10.3390/ijerph121214979