Next Article in Journal
Symbiodinium—Invertebrate Symbioses and the Role of Metabolomics
Previous Article in Journal
Assessing Pearl Quality Using Reflectance UV-Vis Spectroscopy: Does the Same Donor Produce Consistent Pearl Quality?
Open AccessArticle

TFA and EPA Productivities of Nannochloropsis salina Influenced by Temperature and Nitrate Stimuli in Turbidostatic Controlled Experiments

1
Forschungs- und Technologiezentrum Westküste der Universität Kiel, Hafentörn 1, 25761 Büsum, Germany
2
Botanisches Institut der Universität Kiel, Am Botanischen Garten 1–9, 24098 Kiel, Germany
*
Author to whom correspondence should be addressed.
Mar. Drugs 2010, 8(9), 2526-2545; https://doi.org/10.3390/md8092526
Received: 3 September 2010 / Revised: 15 September 2010 / Accepted: 21 September 2010 / Published: 27 September 2010
The influence of different nitrate concentrations in combination with three cultivation temperatures on the total fatty acids (TFA) and eicosapentaenoic acid (EPA) content of Nannochloropsis salina was investigated. This was done by virtue of turbidostatic controlled cultures. This control mode enables the cultivation of microalgae under defined conditions and, therefore, the influence of single parameters on the fatty acid synthesis of Nannochloropsis salina can be investigated. Generally, growth rates decreased under low nitrate concentrations. This effect was reinforced when cells were exposed to lower temperatures (from 26 °C down to 17 °C). Considering the cellular TFA concentration, nitrate provoked an increase of TFA under nitrate limitation up to 70% of the biological dry mass (BDM). In contrast to this finding, the EPA content decreased under low nitrate concentrations. Nevertheless, both TFA and EPA contents increased under a low culture temperature (17 °C) compared to moderate temperatures of 21 °C and 26 °C. In terms of biotechnological production, the growth rate has to be taken into account. Therefore, for both TFA and EPA production, a temperature of 17 °C and a nitrate concentration of 1800 µmol L-1 afforded the highest productivities. Temperatures of 21 °C and 26 °C in combination with 1800 µmol L-1 nitrate showed slightly lower TFA and EPA productivities. View Full-Text
Keywords: microalgae; ratio unsaturated/saturated FA; growth rate; total fatty acids; eicosapentaenoic acid microalgae; ratio unsaturated/saturated FA; growth rate; total fatty acids; eicosapentaenoic acid
MDPI and ACS Style

Hoffmann, M.; Marxen, K.; Schulz, R.; Vanselow, K.H. TFA and EPA Productivities of Nannochloropsis salina Influenced by Temperature and Nitrate Stimuli in Turbidostatic Controlled Experiments. Mar. Drugs 2010, 8, 2526-2545.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop