New Antibacterial Diterpenoids from the South China Sea Soft Coral Klyxum molle

Abstract

Fifteen new diterpenoids, namely xishaklyanes A-O (1–15), along with three known related ones (16–18), were isolated from the soft coral Klyxum molle collected from Xisha Islands, South China Sea. The stereochemistry of the new compounds was elucidated by a combination of detailed spectroscopic analyses, chemical derivatization, quantum chemical calculations, and comparison with the reported data. The absolute configuration of compound 18 was established by the modified Mosher’s method for the first time. In bioassay, some of these compounds exhibited considerable antibacterial activities on fish pathogenic bacteria, and compound 4 showed the most effective activity with MIC of 0.225 μg/mL against Lactococcus garvieae.

1. Introduction

Soft corals of the genus Klyxum (order Alcyonacea, family Alcyoniidae) are broadly distributed over the tropical Indo-Pacific, including the South China Sea [1]. Different from the commonly chemically investigated Sinularia [2] and Sarcophyton [3] soft corals, only three species from the genus Klyxum have been chemically studied, including Klyxum simplex [4,5,6,7], Klyxum molle [8,9,10,11], and Klyxum flaccidum [12,13,14,15]. Diverse secondary metabolites were discovered, including novel skeleton (Klyflaccilides A and B) and eunicellin-type diterpenoids [8,9,10,11,12]. These compounds exhibited widespread biological activities, such as antibacterial, cytotoxic, and anti-inflammatory effects [4,5,6,7,8,9,10,11,12,14,15].

As part of our continuous research project aiming for the discovery of bioactive metabolites from Chinese marine Cnidaria [12,16,17,18], Klyxum molle collected off the Xisha Islands were subjected to systematic research, yielding fifteen new diterpenoids 1−15 and three known related diterpenoids 16−18 (Figure 1). Herein, the isolation, structural elucidation, and biological evaluations of these compounds are reported.

2. Results and Discussion

By a series of column chromatography in combination with HPLC, the acetone extract of K. molle resulted in the purification of fifteen new diterpenoids, namely xishaklyanes A-O (1–15), along with three known related ones (16–18) (Figure 1). Those known diterpenoids were unambiguously identified as fuscol (16) [19], lobovarol H (17) [20], and 17,18-epoxyloba-8,10,13(15)-trien-16-ol (18) [21], respectively, by comparing their NMR data and specific rotation values with those reported in the literature.

Xishaklyane A (1) was obtained as an optically active colourless oil. From the pseudo molecular ion peak at m/z 289.2527 ([M + H]⁺, calcd. 289.2526) in the HRESIMS spectrum, a molecular formula of C₂₀H₃₂O was established, indicating five degrees of unsaturation. The ¹H and ¹³C NMR data of 1 (

Table 1. The ¹H NMR data (600 MHz, δ_H in ppm, J in Hz) for compounds 1–5 in CDCl₃.

No.	1	2	3	4	5
	δH Mult. (J Hz)	δH Mult. (J Hz)	δH Mult. (J Hz)	δH Mult. (J Hz)	δH Mult. (J Hz)
2	2.08, dd (12.9, 3.3)	1.94, dd (11.8, 3.2)	1.94, dd (11.8, 3.2)	1.97, dd (11.8, 3.3)	1.97, dd (11.8, 3.3)
3a	1.68, m	1.43, m	1.43, m	1.50, m	1.50, m
3b	1.74, m	1.59, m	1.59, m	1.62, m	1.62, m
4	2.70, tt (12.1, 3.8)	1.43, m	1.43, m	1.50, m	1.50, m
5a	1.38, m	1.44, m	1.44, m	1.33, m	1.33, m
5b	1.59, m	1.63, m	1.63, m	1.45, m	1.45, m
6a	1.44, m	1.44, m	1.44, m	1.46, m	1.46, m
6b	1.55, m	1.44, m	1.44, m	1.46, m	1.46, m
7	1.02, s	0.97, s	0.97, s	0.97, s	0.97, s
8	5.83, dd (17.2, 11.3)	5.80, dd (15.3, 10.9)	5.80, dd (15.3, 10.9)	5.80, dd (15.3, 10.9)	5.80, dd (15.3, 10.9)
9a	4.90, d (11.3)	4.88, d (10.9)	4.88, d (10.9)	4.88, d (10.9)	4.88, d (10.9)
9b	4.90, d (17.2)	4.89, d (15.3)	4.89, d (15.3)	4.89, d (15.3)	4.89, d (15.3)
11a	4.59, s	4.58, s	4.58, s	4.58, s	4.58, s
11b	4.81, s	4.81, s	4.81, s	4.81, s	4.81, s
12	1.71, s	1.70, s	1.70, s	1.70, s	1.70, s
14	1.76, s	1.29, s	1.29, s	1.35, s	1.35, s
15	5.77, d (10.9)	5.65, d (15.3)	5.65, d (15.3)	5.31, d (11.9)	5.31, d (11.9)
16	6.50, dd (15.2, 10.9)	6.43, dd (15.3, 10.9)	6.43, dd (15.3, 10.9)	6.19, t (11.9)	6.19, t (11.9)
17	5.70, d (15.2)	5.84, d (10.9)	5.84, d (10.9)	6.63, d (11.9)	6.63, d (11.9)
19	1.34, s	1.78, s	1.78, s	1.74, s	1.74, s
20	1.34, s	1.78, s	1.78, s	1.81, s	1.81, s

Chemical shifts (ppm) refer to CHCl₃ (δ_H 7.26). Assignments were deduced by analysis of 1D and 2D NMR spectra.

and

Table 2. The ¹³C NMR data (125 MHz, δ_C in ppm) for compounds 1–10 in CDCl₃.

No.	1	2	3	4	5	6	7	8	9	10
	δC Mult.	δC Mult.	δC Mult.	δC Mult.	δC Mult.	δC Mult.	δC Mult.	δC Mult.	δC Mult.	δC Mult.
1	39.7, C	39.9, C	39.9, C	39.9, C	39.9, C	39.9, C	39.9, C	39.9, C	39.9, C	39.8, C
2	52.6, CH	52.8, CH	52.8, CH	52.7, CH	52.8, CH	52.9, CH	53.0, CH	53.0, CH	52.6, CH	52.8, CH
3	31.9, CH2	28.2, CH2	28.5, CH2	28.1, CH2	28.5, CH2	33.3, CH2	34.3, CH2	34.7, CH2	33.1, CH2	33.9, CH2
4	40.5, CH	48.8, CH	48.9, CH	49.5, CH	49.6, CH	41.1, CH	40.9, CH	41.0, CH	37.2, CH	41.1, CH
5	26.0, CH2	22.7, CH2	22.4, CH2	22.6, CH2	22.2, CH2	27.2, CH2	28.7, CH2	28.2, CH2	27.2, CH2	27.1, CH2
6	39.8, CH2	40.0, CH2	39.9, CH2	40.0, CH2	40.0, CH2	40.0, CH2	40.2, CH2	40.1, CH2	39.7, CH2	39.8, CH2
7	16.8, CH3	16.7, CH3	16.7, CH3	16.7, CH3	16.7, CH3	16.8, CH3	16.8, CH3	16.8, CH3	16.7, CH3	16.7, CH3
8	150.4, CH	150.4, CH	150.4, CH	150.5, CH	150.5, CH	150.2, CH	150.3, CH	150.3, CH	150.1, CH	150.1, CH
9	110.1, CH2	110.0, CH2	110.0, CH2	110.0, CH2	110.0, CH2	110.2, CH2	110.1, CH2	110.1, CH2	110.2, CH2	110.3, CH2
10	147.7, C	148.1, C	148.0, C	148.1, C	148.0, C	147.6, C	147.6, C	147.6, C	147.5, C	147.4, C
11	112.3, CH2	112.2, CH2	112.2, CH2	112.2, CH2	112.2, CH2	112.4, CH2	112.3, CH2	112.4, CH2	112.4, CH2	112.4, CH2
12	25.0, CH3	24.9, CH3	24.9, CH3	24.8, CH3	24.9, CH3	24.9, CH3	24.9, CH3	24.9, CH3	24.9, CH3	25.0, CH3
13	143.1, C	75.0, C	75.1, C	76.7, C	76.7, C	140.2, C	156.0, C	156.0, C	145.7, C	149.2, C
14	20.2, CH3	26.2, CH3	26.2, CH3	27.4, CH3	27.5, CH3	61.6, CH2	109.1, CH2	108.9, CH2	75.3, CH2	109.4, CH2
15	124.6, CH	135.3, CH	135.2, CH	133.5, CH	133.4, CH	128.4, CH	75.0, CH	74.9, CH	120.9, CH	56.0, CH
16	122.0, CH	124.4, CH	124.4, CH	125.5, CH	125.5, CH	122.0, CH	128.1, CH	128.2, CH	83.1, CH	58.5, CH
17	139.3, CH	124.8, CH	124.8, CH	121.4, CH	121.5, CH	143.0, CH	139.6, CH	139.6, CH	125.7, CH	63.0, CH
18	71.1, C	137.0, C	137.0, C	137.3, C	137.3, C	71.1, C	70.8, C	70.8, C	135.5, C	58.6, C
19	30.2, CH3	18.5, CH3	18.5, CH3	17.8, CH3	17.8, CH3	29.9, CH3	29.9, CH3	29.9, CH3	18.2, CH3	19.7, CH3
20	30.2, CH3	26.0, CH3	26.1, CH3	26.7, CH3	26.7, CH3	29.9, CH3	30.0, CH3	29.9, CH3	26.0, CH3	24.7, CH3
21						171.3, C
22						21.2, CH3

Chemical shifts (ppm) refer to CHCl₃ (δ_C 77.2). Assignments were deduced by analysis of 1D and 2D NMR spectra.

) highly resembled those of co-occurring 16, with the only differences in signals on C-4 (δ_C 47.5 in 16 and 40.5 in 1), C-14 (δ_C 15.3 in 16 and 20.2 in 1), and C-15 (δ_C 122.9 in 16 and 124.6 in 1), suggesting that 1 was the analogue of 16, with the opposite geometry on Δ^13,15. The characteristic NOE correlation between H₃-14 and H-15 also confirmed the Z geometry of Δ^13,15 in 1 (Figure 2). The structure of 1 was further confirmed by 2D NMR analysis, including HMBC and NOESY correlations. Thus, compound 1 was determined to be 13Z-fuscol, namely xishaklyane A.

Xishaklyane B (2) and xishaklyane C (3) were initially isolated as a mixture [22], displaying two sets of carbon signals in the ¹³C NMR spectrum. Normal Phase-High Performance Liquid Chromatography (NP-HPLC) CHIRALPAK^® IC (250 mm × 4.6 mm, 5 µm, Daicel Corporation, Osaka, Japan) (n-hexane/isopropanol (99.7:0.3), 1.0 mL/min) (2: t_R = 4.6 min; 3: t_R = 5.5 min) was used to successfully separate the mixture into 2 and 3. The absolute configurations (ACs) of 2 and 3 were further established by TDDFT-ECD calculations, a reliable approach to determine the ACs of natural products with chiral carbons near the chromophore groups [23,24,25,26]. As shown in Figure 3, the Boltzmann-averaged ECD spectrum of (1R,2R,4S,13R,15E)-2 matched to the experimental ECD spectrum of 2, and the Boltzmann-averaged ECD spectrum of (1R,2R,4S,13S,15E)-3 matched to the experimental ECD spectrum of 3. Consequently, the ACs of 2 and 3 were determined to be 1R, 2R, 4S, 13R, 15E, and 1R, 2R, 4S, 13S, 15E, respectively. Thus, xishaklyanes B (2) and C (3) were deduced to be 13R, 15E-isofuscol and 13S, 15E-isofusol, respectively.

Xishaklyane D (4) and xishaklyane E (5) were also isolated as a pair of epimers, which were separated by NP-HPLC (n-hexane/isopropanol (99.2:0.8), 1.0 mL/min) (5: t_R = 6.5 min; 6: t_R = 5.6 min). The 1D and 2D NMR spectra of the mixture (Table 1 and Table 2) showed great similarity to those of the mixture of 2 and 3, with the only differences in the chemical shifts between C-13 and C-17, indicating the opposite geometry of Δ^15,16. The Z geometry of Δ^15,16 was deduced by the ¹H-¹H coupling constants (J = 12.0 Hz). The ACs of 4 and 5 were also determined by TDDFT-ECD calculation. As shown in Figure 4, the Boltzmann-averaged ECD spectrum of (1R,2R,4S,13R,15Z)-4 matched to the experimental ECD spectrum of 4, while the Boltzmann-averaged ECD spectrum of (1R,2R,4S,13S,15Z)-5 matched to the experimental ECD spectrum of 5. Consequently, the ACs of 4 and 5 were determined to be 1R,2R,4S,13R,15Z and 1R,2R,4S,13S,15Z, respectively. Finally, xishaklyane D (4) was determined as 13R,15Z-isofuscol, while xishaklyane E (5) was determined to be 13S,15Z-isofusol.

Xishaklyane F (6) was obtained as an optically active colourless oil. Its molecular formula was deduced to be C₂₂H₃₄O₃ on the basis of HRESIMS (sodiated ion peak at m/z 369.2408 ([M + Na]⁺, calcd. 369.2400)), indicating six degrees of unsaturation. The ¹H and ¹³C NMR data of 6 were reminiscent of those of the known compound 17 (Table 2 and

Table 3. The ¹H NMR data (600 MHz, δ_H in ppm, J in Hz) for compounds 6–10 in CDCl₃.

No.	6	7	8	9	10
	δH Mult. (J Hz)	δH Mult. (J Hz)	δH Mult. (J Hz)	δH Mult. (J Hz)	δH Mult. (J Hz)
2	2.02, dd (10.7, 5.4)	1.99, dd (10.7, 5.4)	1.99, dd (10.7, 5.4)	2.01, dd (12.7, 3.4)	2.02, dd (12.8, 3.2)
3a	1.57, m	1.57, m	1.57, m	1.61, m	1.53, m
3b	1.57, m	1.57, m	1.57, m	1.61, m	1.60, m
4	2.11, m	1.99, m	1.99, m	2.10, t (11.9)	1.99, m
5a	1.48, m	1.47, m	1.47, m	1.44, m	1.50, m
5b	1.63, m	1.62, m	1.62, m	1.68, m	1.62, m
6a	1.48, m	1.45, m	1.45, m	1.46, m	1.48, m
6b	1.51, m	1.51, m	1.51, m	1.51, m	1.51, m
7	1.01, s	1.02, s	1.02, s	1.01, s	1.01, s
8	5.82, dd (17.6, 10.6)	5.81, dd (17.8, 10.5)	5.81, dd (17.8, 10.5)	5.81, dd (17.8, 10.5)	5.81, dd (17.8, 10.5)
9a	4.90, d (10.6)	4.90, d (10.5)	4.90, d (10.5)	4.90, d (10.5)	4.90, d (10.5)
9b	4.90, d (17.6)	4.91, d (17.8)	4.91, d (17.8)	4.90, d (17.8)	4.92, d (17.8)
11a	4.58, s	4.58, s	4.58, s	4.58, s	4.57, s
11b	4.82, s	4.82, s	4.82, s	4.83, s	4.83, s
12	1.70, s	1.70, s	1.70, s	1.71, s	1.71, s
14a	4.75, d (3.3)	4.99, s	4.99, s	4.54, dddd (11.9, 3.4, 2.3, 0.9)	4.95, s
14b		5.15, s	5.15, s	4.64, dddd (11.8, 5.3, 2.1, 0.9)	5.09, s
15	6.10, d (11.0)	4.62, m	4.62, m	5.31, m	3.31, d (2.2)
16	6.55, dd (15.2, 11.0)	5.66, dd (15.6, 6.5)	5.66, dd (15.6, 6.5)	5.50, dtd (8.9, 3.7, 1.8)	2.73, dd (5.9, 2.2)
17	5.89, d (15.2)	5.90, d (15.6)	5.90, d (15.6)	5.15, dt (8.9, 1.4)	2.63, d (5.9)
19	1.35, s	1.33, s	1.33, s	1.73, s	1.35, s
20	1.35, s	1.33, s	1.33, s	1.73, s	1.39, s
22	2.07, s

Chemical shifts (ppm) refer to CHCl₃ (δ_H 7.26). Assignments were deduced by analysis of 1D and 2D NMR spectra.

), with the only difference on C-14 (δ_H 4.29, 4.31, δ_C 60.0 in 17, and δ_H 4.75, 4.75, δ_C 61.6 in 6), as well as an additional acetyl group in 6 (δ_H 2.07 s, δ_C 21.2, 171.3), indicating the acetylation of 14-OH of 17 towards 6. To confirm our deduction, the acetylation of 17 (3 mg) was carried out using pyridine and Ac₂O at room temperature for 24 h, yielding the acetate 6 (2 mg). Thus, compound 6 was determined as the 14-acetate of the known compound lobovarol H (17), namely xishaklyane F.

Xishaklyanes G and H (7 and 8) were initially obtained as a mixture, which were further separated by NP-HPLC (n-hexane/isopropanol (90:10), 0.9 mL/min) (7: t_R = 6.4 min; 8: t_R = 5.6 min). They showed the same pseudo molecular ion peak at m/z 303.2329 ([M − H]⁻, calcd. 303.2330) in the HRESIMS spectrum, and owned the same molecular formula of C₂₀H₃₂O₂, indicating compound 7 to be isomeric with 8. Detailed analysis of their NMR data suggested an epimeric relationship between 7 and 8. The ¹H and ¹³C NMR data of 7 and 8 (Table 2 and Table 3) showed difference at C-15 and its neighbouring carbons (e.g., C-3, C-5 and C-14), indicating 7 and 8 may have the opposite configuration of 15-OH. For the planar structure of both compounds, taking 7 for an example, its ¹H and ¹³C NMR and HSQC resonances, as well as its coupling constants of the connected protons, indicated the presence of one monosubstituted terminal double bond [δ_H 5.81 (dd, J = 17.8, 10.5 Hz), 4.91 (d, J = 17.8 Hz), 4.90 (d, J = 10.5 Hz), δ_C 110.1 (t), δ_C 150.3 (s)], two disubstituted terminal olefinic bond [δ_H 4.82 (s), δ_H 4.58 (s), δ_C 112.4 (t), δ_C 147.7 (s); δ_H 4.99 (s), δ_H 5.15 (s), δ_C 109.0 (t), δ_C 156.0 (s)], and two monosubstituted double bond [δ_H 5.66 (dd, J = 15.6, 6.5 Hz), δ_H 5.90 (d, J = 15.6 Hz), δ_C 128.2 (d), δ_C 139.7 (d)]. The above olefinic bonds accounted for four degrees of unsaturation, so the remaining one degree should be ascribed to a ring in the molecule. Further analysis of ¹H-¹H COSY spectrum of compound 7 revealed three structural fragments a–c. These fragments were connected with well resolved HMBC correlations from H₃-7 to C-1/C-2/C-6/C-8, from H₃-12 to C-2/C-10/C-11, from H₃-19/H₃-20 to C-17/C-18, and from H₂-14 to C-4/C-13/C-15 (Figure 2). Consequently, the planar structure of 7 was identified as shown in Figure 1, featuring a lobane-type diterpenoid skeleton. The determination of the relative configurations (RCs) of 7 and 8 were highly challenging, because of the chiral carbon on the side chain, whose RCs cannot be elucidated by only a NOESY experiment. The E geometry of Δ^16,17 was deduced by the ¹H-¹H coupling constants (J = 15.6 Hz). NOE correlations of H-4/H-3β/H-2 revealed that these protons were disposed at the same side of the molecule and randomly assigned as β-oriented. The correlation of H₃-7/H-3α revealed that these protons and proton-bearing groups were positioned at the other side of the molecule and were thus α-directed (Figure 2). The ACs of 7 and 8 were determined by the TDDFT-ECD calculation. As shown in Figure 4, the Boltzmann-averaged ECD spectrum of (1R,2R,4S,15R,16E)-7 matched to the experimental ECD spectrum of 7, while the Boltzmann-averaged ECD spectrum of (1R,2R,4S,15S,16E)-8 matched to the experimental ECD spectrum of 8. Consequently, the absolute configuration of 7 was determined to be 1R,2R,4S,15R,16E. The absolute configuration of 8 was determined to be 1R,2R,4S,15S,16E.

Xishaklyane I (9) was obtained as an optically active colourless oil. Its molecular formula of C₂₀H₃₀O was determined from the molecular ion peak at m/z 286.2288 ([M]⁺, calcd. 286.2291) in the HREIMS spectrum, indicating six degrees of unsaturation. Under detailed diagnostic 2D NMR spectra, as well as coupling constants of the connected protons (Table 2 and Table 3), compound 9 owned the same skeleton as the previously mentioned compounds. The major differences between them mainly happened at the C-14 position. An ether bridge between C-14 and C-16 formed a furan ring, which can be further verified by HMBC correlation from H-16 to C-14. As for the relative stereochemistry of 9, the relative configurations at C-1, C-2, and C-4 were suggested to be the same as those of 1–8, which was supported by the similar NOE relationships observed in the NOESY spectrum (Figure 2). Therefore, there are only two possibilities for the RC of 9 ((1R*, 2R*, 4S*, 16R*)-9 and (1R*, 2R*, 4S*, 16S*)-9). Thus, the QM-NMR calculation and DP4+ analyses [27,28,29] of the ¹³C NMR parameter on the two possible candidate diastereoisomers were performed. Finally, the experimentally observed ¹³C NMR data for 9 gave its best match for the 1R*, 2R*, 4S*, 16R* isomer (9a, see the details in Supplementary Materials), with a 99.83% probability. Like the AC of fuscol, the absolute configuration of 9 was determined to be 1R,2R,4S,13Z,16R.

The molecular formula C₂₀H₃₀O₂ of xishaklyane J (10) was deduced by the HRESIMS pseudo molecular ion peak at m/z 303.2315 ([M + H]⁺ calcd. 303.2319), implying six degrees of unsaturation. The ¹H and ¹³C NMR data of 10 (Table 2 and Table 3) were closely reminiscent of those of the co-occurrent 17,18-epoxyloba-8,10,13(15)-trien-16-ol (18). The only difference between them was the presence of a terminal alkene (C-13/C-14) and an epoxide (C-15/C-16) in 10 instead of a trisubstituted double bond (C-13/C-15) and a hydroxyl (C-16) in 18. The detailed 2D NMR analysis shown in Figure 2 confirmed its planar structure. To further confirm the structure and RC of 10, the QM-NMR calculation and DP4+ analyses were used. Finally, the experimentally observed NMR data for 10 gave its best match for the 1R*, 2R*, 4S*, 16S*, 17R*, 18R* isomer (10d, see the details in Supplementary Materials), with a 100.00% probability. Like the AC of fuscol, the absolute configuration of 10 was determined to be 1R,2R,4S,16S,17R,18R.

Biogenetically, 10 was believed to be derived from compound 18 by an acid induced electron delivery from 16-OH to first generate the 15,16-epoxyl and then promote the double bond migration towards the terminal olefin. The AC of the known compound 17,18-epoxyloba-8,10,13(15)-trien-16-ol (18) has not been defined. To obtain its absolute configuration at C-16, two aliquots of compound 18 were treated with (R)- and (S)-α-methoxy-α-trifluoromethylphenyl acetyl (MTPA) chlorides to obtain the (S)- and (R)-esters, respectively. Analysis of Δδ^SR values (δ_S–δ_R) observed for the signals of the protons close to 16-OH indicated the R configuration at this carbon (Figure 5).

Xishaklyane K (11) was obtained as an optically active colourless oil. Its molecular formula was deduced to be C₂₀H₃₂O on the basis of the HREIMS (molecular ion peak at m/z 288.2443 ([M]⁺, calcd. 288.2448)). Careful analysis of the 1D NMR spectra of 11 (

Table 4. The ¹H NMR data (600 MHz, δ_H in ppm, J in Hz) for compounds 11–15 in CDCl₃.

No.	11	12	13	14	15
	δH Mult. (J Hz)	δH Mult. (J Hz)	δH Mult. (J Hz)	δH Mult. (J Hz)	δH Mult. (J Hz)
1a	1.28, m	1.10, m	3.49, dd (9.8, 5.9)	1.52, m	1.72, m
1b	1.44, m	1.39, m		1.57, m	1.83, m
2a	1.59, m	1.55, m	1.53, m	1.55, m	1.72, m
2b	1.64, m		1.70, m	1.82, m	1.76, m
3a	2.00, m	1.37, m	1.97, m	2.30, m
3b	2.31, m	1.79, m	2.17, m	2.39, m
4					1.79, m
5a	1.82, d (12.4)	1.25, m		3.10, ddd (8.2, 3.2, 0)	1.52, m
5b					1.83, m
6a	1.34, m	1.50, m	1.84, m	1.71, m	2.44, m
6b	1.52, m	1.50, m	2.52, dt (12.7, 2.1)	1.86, m
7a	2.00, m	1.99, tt (9.7, 4.1)	1.84, m	2.30, m	1.64, m
7b					1.66, m
8a	1.50, m	1.24, m	1.53, m	1.72, m	2.12, ddd (14.3, 9.7, 4.0)
8b	1.51, m	1.82, m	1.70, m		2.49, ddd (14.3, 6.7, 3.5)
9a	1.28, m	1.21, m1.44, m	1.21, td (13.2, 3.4)
9b	1.51, m		2.04, t (3.4)
10				2.02, ddd (12.6, 10.7, 3.2)	2.27, m
11a	0.73, s	0.89, s	1.03, s	1.15, s	4.70, s
11b					4.71. s
12a	4.42, q (1.8)	1.11, s	1.59, s	4.75, s	1.18, s
12b	4.70, q (1.8)			4.87, s
14	1.80, s	1.79, s	1.73, s	1.77, s	1.75, s
15	5.88, d (10.8)	5.87, d (10.8)	5.33, d (8.8)	5.87, d (10.7)	5.90, d (10.7)
16	6.50, dd (15.3, 10.8)	6.48, dd (15.3, 10.8)	4.26, dd (8.8, 7.8)	6.46, dd (15.3, 10.7)	6.45, dd (15.3, 10.7)
17	5.75, d (15.3)	5.75, d (15.3)	2.84, d (7.8)	5.75, d (15.3)	5.75, d (15.3)
19	1.36, s	1.35, s	1.33, s	1.36, s	1.35, s
20	1.36, s	1.35, s	1.35, s	1.36, s	1.35, s

Chemical shifts (ppm) refer to CHCl₃ (δ_H 7.26). Assignments were deduced by analysis of 1D and 2D NMR spectra.

and

Table 5. The ¹³C NMR data (125 MHz, δ_C in ppm) for compounds 11–15 in CDCl₃.

No.	11	12	13	14	15
	δC Mult.	δC Mult.	δC Mult.	δC Mult.	δC Mult.
1	42.1, CH2	41.2, CH2	78.5, CH	29.0, CH2	26.4, CH2
2	23.6, CH2	20.3, CH2	27.3, CH2	30.0, CH2	40.8, CH2
3	37.0, CH2	43.5, CH2	32.1, CH2	34.8, CH2	81.1, C
4	151.1, C	72.4, C	124.4, C	151.5, C	52.7, CH
5	50.0, CH	55.0, CH	133.4, C	45.3, CH	31.5, CH2
6	29.4, CH2	26.7, CH2	30.8, CH2	26.4, CH2	45.6, CH
7	48.0, CH	48.5, CH	47.7, CH	44.0, CH	31.2, CH2
8	26.7, CH2	26.0, CH2	27.3, CH2	40.7, CH2	37.0, CH2
9	41.3, CH2	44.7, CH2	38.9, CH2	81.8, C	153.4, C
10	36.1, C	34.7, C	39.6, C	48.6, CH	48.4, CH
11	16.5, CH3	18.9, CH3	17.5, CH3	24.1, CH3	107.2, CH2
12	105.6, CH2	22.9, CH3	19.2, CH3	109.3, CH2	24.0, CH3
13	144.1, C	143.8, C	145.9, C	143.2, C	143.9, C
14	15.4, CH3	15.5, CH3	15.9, CH3	16.1, CH3	14.7, CH3
15	122.4, CH	122.5, CH	120.9, CH	122.8, CH	123.0, CH
16	123.4, CH	123.3, CH	67.9, CH	123.4, CH	123.2, CH
17	139.3, CH	139.3, CH	67.6, CH	139.5, CH	139.5, CH
18	71.1, C	71.1, C	60.0, C	71.1, C	71.1, C
19	30.1, CH3	30.1, CH3	19.7, CH3	30.1, CH3	30.1, CH3
20	30.1, CH3	30.1, CH3	25.1, CH3	30.1, CH3	30.1, CH3

Chemical shifts (ppm) refer to CHCl₃ (δ_C 77.2). Assignments were deduced by analysis of 1D and 2D NMR spectra.

) showed a close similarity with those of co-occurring 16, indicating 11 as also being a same side carbon chain at C-7. Further analysis of its 1D and 2D NMR spectra revealed that the main difference was between C-1 and C-12. The ¹H-¹H COSY correlation of H₂-2/H₂-3 formed a six-membered ring, which can also be deduced from the HMBC correlation from H₂-3 to C-1. Therefore, the planar structure was identified, as shown in Figure 1, as a prenyleudesmane type diterpene. The remaining task was to determine the structure and RC of 11. The E geometry of Δ^16,17 was deduced by the ¹H-¹H coupling constants (J = 15.3 Hz) and the E geometry of Δ^13,15 was deduced by the NOE correlations between H-15 and H-7 (Figure 6). The relative configuration of chiral centres C-10, C-5, and C-7 in compound 11 was the same as those compounds mentioned above. To determine the AC of 11, a TDDFT-ECD calculation was performed. As shown in Figure 4, the Boltzmann-averaged ECD spectrum of (5S,7R,10S)-11 matched to the experimental ECD spectrum of 11. Consequently, the AC of 11 was determined to be 5S,7R,10S,13E,16E.

Xishaklyane L (12) was obtained as an optically active colourless oil. From the molecular ion peak at m/z 306.2553 ([M]⁺, calcd. 306.2553) in the HREIMS spectrum, a molecular formula of C₂₀H₃₂O₂ was established. The structural features of 12 were reminiscent of the known compound lobovarol K [20]. Compound 12 was methylated at the hydroxyl of C-18 to form lobovarol K. The same NOE correlations (Figure 6) meant that the structure of 12 was tentatively determined to be the same as lobovarol K.

Xishaklyane M (13) has a molecular formula C₂₀H₃₂O₃, as displayed from the ion peak in the HREIMS (m/z 320.2347 [M]⁺). The 1D and 2D NMR data of 13 (Table 4 and Table 5) further revealed that it was a diterpene possessing the same double rings with identical substitutions as that of the eudesmane derivative. The NOE correlations between H₃-11 and H-6α/H-8α, H-6α and H-8α, H-1, and H-9α, and between H-7 and H-9β, suggested the β-orientation of H-7, and the α-orientation of H₃-11 and H-1, respectively (Figure 6). The side chain was established by detailed 1D and 2D NMR data of 13, indicating a hydroxyl at C-16 and an epoxide at C-17/C-18, which is the same as the known compound 18. Comparing their NMR data, the absolute configuration of 13 was tentatively determined to be 1R,4Z,7S,10S,13E,16R,17R.

Xishaklyane N (14) displayed a molecular formula of C₂₀H₃₂O₂ as established by the HREIMS ion peak at m/z 304.2400 ([M]⁺ calcd for 304.2397). The ¹H and ¹³C NMR data of 14 (Table 4 and Table 5) were very similar with 11. Detailed analysis of those spectra of 14, and comparing with those of 11, revealed that the main differences between them happened at C-9 to C-11 segments. The HMBC correlations from H₃-11 to three carbons (C-8/C-9/C-10) instead of four carbons (C-5/C-8/C-9/C-10) in 11 indicated that a methyl was displaced at C-9. As for the stereochemistry of compound 14, the chemical shifts of C-14 (δ_C 15.4, CH₃) and J value of H-16 and H-17 (15.3 Hz) indicated the E-geometry for the 13,15- and 16,17-double bonds, respectively. The NOE correlations between H₃-11 and H-10, H-5 and H-10, H-5 and H-6β, and between H-7 and H-6α, suggested the cis-decahydronaphthalene core structure, where H-7 and H-10 are axial orientation while H-5 and Me-9 are equatorial orientation (Figure 6). RC of 14: 5S*, 7S*, 9R*, 10S*. As shown in Figure 4, the Boltzmann-averaged ECD spectrum of (5S,7S,9R,10S)-14 matched to the experimental ECD spectrum of 14. Consequently, the AC of 14 was determined to be 5S,7S,9R,10S,13E,16E.

Xishaklyane O (15) was also obtained as an optically active colourless oil. From the molecular ion peak at m/z 304.2402 ([M]⁺, calcd. 304.2397) in the HREIMS spectrum, a molecular formula of C₂₀H₃₂O₂ was established, indicating five degrees of unsaturation. Detailed analysis of 1D and 2D NMR spectra (Table 4 and Table 5) revealed that it had the same side chain as compounds 11, 12, 14, and 16. Further analysis of ¹H-¹H COSY and the HMBC spectrum of 15 revealed a 5/7-fused carbon ring system (Figure 6). As for the stereochemistry of compound 15, the E geometry of Δ^16,17 was deduced by the ¹H-¹H coupling constants (J = 15.3 Hz), and the E geometry of Δ^13,15 was deduced by the chemical shifts of C-14 (δ_C 14.9, CH₃). The NOE correlations between H-5β and H-10/H-6, H-5α and H-4, H-10 and H-6, and between H₃-12 and H-10, suggested the α-orientation of H-10, H-4, and H₃-12, and the β-orientation of H-6, respectively. RC of 15: 3R*, 4R*, 6S*, 10S*. As shown in Figure 4, the Boltzmann-averaged ECD spectrum of (3R,4R,6S,10S)-15 matched to the experimental ECD spectrum of 15. Consequently, the absolute configuration of 15 was determined to be 3R,4R,6S,10S,13E,16E.

All compounds were screened for antibacterial activities on fish pathogenic bacteria. As shown in

Table 6. Antibacterial activities on fish pathogenic bacteria of compounds 1–6, 10,11, 16–18.

Compd.	MIC (μg/mL)
	Streptococcus parauberis	Lactococcus garvieae	Streptococcus parauberis	Photobacterium damselae	Aeromonas salmonicida
	FP KSP28	MP5245	SP0F3K	FP2244	AS42
1	14.4	28.8	7.2	7.2	NA
2	7.2	NA	28.8	14.4	NA
3	7.2	NA	NA	14.4	28.8
4	7.2	0.225	NA	28.8	NA
5	7.2	28.8	NA	28.8	NA
6	17.3	NA	17.3	17.3	NA
10	15.1	NA	NA	NA	NA
11	0.9	14.4	NA	7.2	28.8
16	7.2	14.4	3.6	7.2	NA
17	NA	NA	NA	NA	NA
18	7.6	NA	NA	15.2	NA
Tetr	3.01	0.38	>24.05	0.02	6.11
Oxy	1.55	0.19	12.42	0.02	0.39
Lev	1.24	0.62	1.24	0.02	0.31
AMP	4.64	0.58	0.58	0.02	>18.57

Tetr = Tetracycline, Oxy = Oxytetracycline hydrochloride, Lev = Levofloxacin hydrochloride. and AMP = Ampicillin sodium, used as positive control; NA = not active at 30 μg/mL; significant (MIC ≤ 10 μg/mL), moderate (10 < MIC ≤ 100 μg/mL), and low or negligible (MIC > 100 μg/mL) [30,31].

, some of them exhibited considerable antibacterial activities. Among them, compound 4 was the most effective one, with an MIC of 0.225 μg/mL against Lactococcus garvieae, whereas compound 11 showed the best antibacterial activity against Streptococcus parauberis, with an MIC of 0.9 μg/mL.

3. Materials and Methods

3.1. General Experimental Procedures

Optical rotations were measured on a Perkin-Elmer 241MC polarimeter (PerkinElmer, Fremont, CA, USA). UV and CD spectra were recorded on a Jasco J-815 spectropolarimeter (JASCO, Tokyo, Japan) at ambient temperature using chromatographic grade CH₃OH and CH₃CN as solvents. IR spectra were recorded on a Nicolet 6700 spectrometer (Thermo Scientific, Waltham, MA, USA); peaks are reported in cm⁻¹. The NMR spectra were measured at 300 K on Bruker Avance III 400, 500, or 600 MHz NMR spectrometers (Bruker Biospin AG, Fällanden, Germany). Chemical shifts are reported in parts per million (δ) in CDCl₃ (δ_H reported referred to CHCl₃ at 7.26 ppm; δ_C reported referred to CDCl₃ at 77.16 ppm) and coupling constants (J) in Hz; assignments were supported by ¹H-¹H COSY, HSQC, HMBC, and NOESY experiments. HREIMS data were recorded on a Finnigan-MAT-95 mass spectrometer (Finnigan-MAT, San Jose, CA, USA). HRESIMS spectra were recorded on an Agilent G6520 Q-TOF mass spectrometer (Agilent, Santa Clara, CA, USA). Semi-preparative RP-HPLC was performed on an Agilent-1260 system (Agilent, Santa Clara, CA, USA) equipped with a DAD G1315D detector at 210 and 254 nm using XDB-C18 column (250 mm × 9.4 mm, 5 µm) by eluting with CH₃OH-H₂O or CH₃CN-H₂O system at 3 mL/min. NP-HPLC was performed on a Shimadzu LC-6A system (Shimadzu, Kyoto, Japan) equipped with a DAD SPD-M20A detector using CHIRALPAK^® IA or CHIRALPAK^® IC (250 mm × 4.6 mm, 5 µm, Daicel Corporation, Osaka, Japan) by eluting with n-hexane-isopropanol system at 1 mL/min. Commercial silica gel (100–200, 200–300, and 300–400 mesh; Qingdao Haiyang Chemical Group Co., Ltd., Qingdao, China) was used for column chromatography (CC). Precoated silica gel GF254 plates (Sinopharm Chemical Reagent Co., Shanghai, China) were used for analytical TLC. Spots were detected on TLC under UV light or by heating after spraying with anisaldehyde H₂SO₄ reagent. Sephadex LH-20 (Amersham Biosciences, Little Chalfont, UK) was also used for CC. All solvents used for column chromatography and HPLC were of analytical grade (Shanghai Chemical Reagents Co., Ltd., Shanghai, China) and chromatographic grade (Dikma Technologies Inc., Foothill Ranch, CA, USA), respectively. The antibiotics tetracycline, oxytetracycline hydrochloride, levofloxacin hydrochloride, and ampicillin sodium were purchased from Sigma-Aldrich^® (Darmstadt, Germany).

3.2. Animal Material

Specimens of the soft coral Klyxum molle were collected by scuba diving at a depth of −20 m in Xisha Islands, Hainan Province, China, in 2019, and were identified by Professor Xiu-Bao Li (Hainan University, Hainan, China). The biological material was frozen immediately after collection. A voucher specimen (19-XS-41) is available for inspection at the Shanghai Institute of Materia Medica, Chinese Academy of Sciences.

3.3. Extraction and Isolation

The frozen animals (868 g, dry weight) of K. molle were cut into pieces and extracted exhaustively with acetone at room temperature (3 × 3.0 L). The organic extract was evaporated to give a brown residue, which was then partitioned between Et₂O and H₂O. The upper layer was concentrated under reduced pressure to give a brown residue (43.3 g), which was fractioned by gradient silica gel (200–300 mesh) column chromatography (CC) (0~100% Et₂O in petroleum ether (PE)), yielding 11 fractions (A–K). Fr. D was fractioned by Sephadex LH-20 (PE/CH₂Cl₂/MeOH, 2:1:1) to obtain three sub-fractions Fr. Da, Db, and Dc. The subfraction Dc was separated on a column of silica gel (10~20% Et₂O in PE) to afford 16 (1.31 g) and 1 (0.26 g). Fr. C was fractioned by Sephadex LH-20 (PE/CH₂Cl₂/MeOH, 2:1:1) to obtain four sub-fractions, Fr. Ca, Cb, Cc and Cd. The subfraction Cd was separated on a column of silica gel (5~20% Et₂O in PE) to afford mixtures Cd2 and Cd6. Cd2 was further purified by RP-HPLC (MeOH/H₂O (90:10), 3.0 mL/min) to give 10 (3.9 mg, t_R = 11.0 min) and Cd2c (0.7 mg, t_R = 11.8 min). Cd6 was further purified by RP-HPLC (MeOH/H₂O (95:5), 3.0 mL/min) to give 11 (1.1 mg, t_R = 8.0 min) and Cd6a (3.1 mg, t_R = 5.3 min). Cd2c was purified by NP-HPLC (n-hexane/isopropanol (99.2:0.8), 1.0 mL/min) to give 4 (0.5 mg, t_R = 6.5 min) and 5 (0.2 mg, t_R = 5.6 min). Cd6a was purified by NP-HPLC (n-hexane/isopropanol (99.7:0.3), 1.0 mL/min) to give 2 (1.4 mg, t_R = 4.6 min) and 3 (1.4 mg, t_R = 5.5 min). Fr. G was fractioned by Sephadex LH-20 (PE/CH₂Cl₂/MeOH, 2:1:1) to obtain three sub-fractions, Fr. Ga, Gb, and Gc. Gb was further purified by RP-HPLC (MeOH/H₂O (85:15), 3.0 mL/min) to give 17 (0.9 mg, t_R = 8.3 min) and 18 (3.7 mg, t_R = 13.7 min). Gc was further purified by RP-HPLC (MeCN/H₂O (60:40), 3.0 mL/min) to give 9 (0.8 mg, t_R = 10.4 min) and 13 (0.6 mg, t_R = 5.3 min). Fr. H was fractioned by Sephadex LH-20 (PE/CH₂Cl₂/MeOH, 2:1:1) to obtain three sub-fractions, Fr. Ha, Hb, and Hc. The subfraction Hc was separated on a column of silica gel (50~75% Et₂O in PE) to afford 6 (15.0 mg) and mixtures Hc1 and Hc2. Hc2 was further purified by RP-HPLC (MeCN/H₂O (70:30), 3.0 mL/min) to give 14 (2.0 mg, t_R = 9.3 min). Hc1 was further purified by RP-HPLC (MeCN/H₂O (55:45), 2.0 mL/min) to give 12 (7.0 mg, t_R = 21.3 min), 15 (1.0 mg, t_R = 18.2 min), and Hc1d (1.3 mg, t_R = 23.6 min). Hc1d was purified by NP-HPLC (n-hexane/isopropanol (90:10), 0.9 mL/min) to give 7 (0.5 mg, t_R = 6.4 min) and 8 (0.7 mg, t_R = 5.6 min).

3.4. Spectroscopic Data of Compounds

Xishaklyane A (1): Colourless oil; [α${]}_D^{20}$ −43.1 (c 1.95, CHCl₃); UV (MeCN) λ_max (log ε) 240 (3.31) nm; ECD (CH₃CN) λ_max (Δε) 215 (−5.2) nm; IR (KBr) ν_max 3382, 2969, 2928, 2860, 1637, 1440, 1376, 1148,1005 cm⁻¹; HRESIMS [M + H]⁺ m/z 289.2527 (calcd. for 289.2526, C₂₀H₃₃O).

Xishaklyane B (2): Colourless oil; [α${]}_D^{20}$ –7.4 (c 0.14, CHCl₃); UV (MeCN) λ_max (log ε) 238 (3.23) nm; ECD (CH₃CN) λ_max (Δε) 201 (−1.6) nm.

Xishaklyane C (3): Colourless oil; [α${]}_D^{20}$ +30.2 (c 0.14, CHCl₃); UV (MeCN) λ_max (log ε) 238 (3.27) nm; ECD (CH₃CN) λ_max (Δε) 232 (+2.3) nm.

Xishaklyane D (4) and Xishaklyane E (5): For 4, Colourless oil; [α${]}_D^{20}$ −35.0 (c 0.05, CHCl₃); UV (MeCN) λ_max (log ε) 240 (3.29) nm; ECD (CH₃CN) λ_max (Δε) 240 (−3.4) nm; For 5, Colourless oil; [α${]}_D^{20}$ +75.8 (c 0.02, CHCl₃); UV (MeCN) λ_max (log ε) 240 (3.35) nm; ECD (CH₃CN) λ_max (Δε) 244 (+3.8) nm; For mixture of 4 and 5, IR (KBr) ν_max 3455, 2966, 2925, 2854, 1438, 1376, 1180, 1142, 1099, 1075,1029 cm⁻¹; HREIMS [M]⁺ m/z 288.2454 (calcd. for 288.2448, C₂₀H₃₂O).

Xishaklyane F (6): Colourless oil; [α${]}_D^{20}$ +33.5 (c 0.09, CHCl₃); UV (MeCN) λ_max (log ε) 239 (3.30) nm; ECD (CH₃CN) λ_max (Δε) 234 (+2.9) nm; IR (KBr) ν_max 3451, 2968, 2925, 2854, 1735, 1377, 1260, 1230, 1075,1027 cm⁻¹; HRESIMS [M + Na]⁺ m/z 369.2408 (calcd. for 369.2400, C₂₂H₃₄NaO₃).

Xishaklyane G (7) and Xishaklyane H (8): For 7, Colourless oil; [α${]}_D^{20}$ +25.3 (c 0.05, CHCl₃); ECD (CH₃CN) λ_max (Δε) 196 (−4.4) nm; For 8, White solid; [α${]}_D^{20}$ +24.0 (c 0.07, CHCl₃); ECD (CH₃CN) λ_max (Δε) 204 (+5.6) nm; For mixture of 7 and 8, IR (KBr) ν_max 3443, 2968, 2924, 2853, 1384, 1180, 1143, 1095, 1076, 1029 cm⁻¹; HRESIMS [M − H]⁻ m/z 303.2329 (calcd. for 303.2330, C₂₀H₃₁O₂).

Xishaklyane I (9): Colourless oil; [α${]}_D^{20}$ +67.9 (c 0.08, CHCl₃); IR (KBr) ν_max 3451, 2967, 2925, 2854, 1444, 1374, 1180, 1059, 1029 cm⁻¹; HREIMS [M]⁺ m/z 286.2288 (calcd. for 286.2291, C₂₀H₃₀O).

Xishaklyane J (10): Colourless oil; [α${]}_D^{20}$ +2.8 (c 0.09, CHCl₃); IR (KBr) ν_max 3454, 2965, 2926, 2856, 1643, 1456,1379, 1075, 1029 cm⁻¹; HRESIMS [M + H]⁺ m/z 303.2315 (calcd. for 303.2319, C₂₀H₃₁O₂).

Xishaklyane K (11): Colourless oil; [α${]}_D^{20}$ −20.0 (c 0.11, CHCl₃); UV (MeCN) λ_max (log ε) 240 (3.34) nm; ECD (CH₃CN) λ_max (Δε) 200 (+2.0) nm; IR (KBr) ν_max 3451, 2869, 2925, 2852, 1442, 1386, 1180, 1143, 1075, 1030 cm⁻¹; HREIMS [M]⁺ m/z 288.2443 (calcd. for 288.2448, C₂₀H₃₂O).

Xishaklyane L (12): Colourless oil; [α${]}_D^{20}$ +25.7 (c 0.70, CHCl₃); UV (MeCN) λ_max (log ε) 240 (3.33) nm; ECD (CH₃CN) λ_max (Δε) 242 (+2.1) nm; IR (KBr) ν_max 3385, 2970, 2926, 2864, 1456, 1384, 1143, 1105 cm⁻¹; HREIMS [M]⁺ m/z 306.2553 (calcd. for 306.2553, C₂₀H₃₄O₂).

Xishaklyane M (13): Colourless oil; [α${]}_D^{20}$ −13.3 (c 0.06, CHCl₃); IR (KBr) ν_max 3450, 2963, 2925, 2854, 1436, 1378, 1180, 1075, 1028 cm⁻¹; HREIMS [M]⁺ m/z 320.2347 (calcd. for 320.2346, C₂₀H₃₂O₃).

Xishaklyane N (14): Colourless oil; [α${]}_D^{20}$ −15.5 (c 0.20, CHCl₃); UV (MeCN) λ_max (log ε) 239 (3.34) nm; ECD (CH₃CN) λ_max (Δε) 210 (−1.9) nm; IR (KBr) ν_max 3450, 2963, 2925, 2857, 1386, 1180, 1143, 1095, 1075, 1028 cm⁻¹; HREIMS [M]⁺ m/z 304.2400 (calcd. for 304.2397, C₂₀H₃₂O₂).

Xishaklyane O (15): Colourless oil; [α${]}_D^{20}$ −16.8 (c 0.10, CHCl₃); UV (MeCN) λ_max (log ε) 240 (3.38) nm; ECD (CH₃CN) λ_max (Δε) 197 (+4.2) nm; IR (KBr) ν_max 3450, 2959, 2923, 2852, 1384, 1180, 1143, 1129, 1099, 1075, 1029 cm⁻¹; HREIMS [M]⁺ m/z 304.2402 (calcd. for 304.2397, C₂₀H₃₂O₂).

3.5. Esterification of 18 with MTPA Chlorides

Compound 18 (2.0 mg) was dissolved in dry pyridine (1000 μL), and the solution was transferred into two NMR tubes (500 μL each), treated with (R)-(−)-2-methoxy-2-(trifluoromethyl) phenylacetyl chloride ((R)-(−)-MTPA-Cl) (20 μL) and (S)-(+)-2-methoxy-2-(trifluoromethyl) phenylacetyl chloride ((S)-(+)-MTPA-Cl) (20 μL), respectively. They were carefully shaken and then monitored immediately by ¹H NMR. The reaction was found to be completed in 30 min. Then the solutions were evaporated in vacuo and the residue was purified by silica gel CC (10% Et₂O in PE) to obtain the S-MTPA ester 18s, and R-MTPA ester 18r, respectively. For (S)-MTPA ester of 18 (18s), Selected ¹H NMR (CDCl₃, 400 MHz): δ_H 5.812 (1H, dd, J = 17.6, 10.7 Hz, H-8), 5.626 (1H, t, J = 9.2 Hz, H-16), 5.359 (1H, d, J = 9.8 Hz, H-15), 4.917 (1H, d, J = 15.9 Hz, H-9α), 4.912 (1H, d, J = 12.4 Hz, H-9β), 4.842 (1H, s, H-11α), 4.585 (1H, s, H-11β), 2.997 (1H, d, J = 8.4 Hz, H-17), 2.009 (1H, d, J = 12.2 Hz, H-4), 1.827 (3H, s, Me-14), 1.713 (3H, s, Me-12), 1.338 (3H, s, Me-20), 1.323 (3H, s, Me-19), 1.013 (3H, s, Me-7). For (R)-MTPA ester of 18 (18r), Selected ¹H NMR (CDCl₃, 400 MHz): δ_H 5.807 (1H, dd, J = 17.6, 10.6 Hz, H-8), 5.591 (1H, t, J = 9.2 Hz, H-16), 5.148 (1H, d, J = 10.1 Hz, H-15), 4.911 (1H, d, J = 16.6 Hz, H-9α), 4.910 (1H, d, J = 11.5 Hz, H-9β), 4.848 (1H, s, H-11α), 4.579 (1H, s, H-11β), 2.996 (1H, d, J = 8.6 Hz, H-17), 1.995 (1H, d, J = 11.9 Hz, H-4), 1.856 (3H, s, Me-14), 1.713 (3H, s, Me-12), 1.358 (3H, s, Me-20), 1.331 (3H, s, Me-19), 0.997 (3H, s, Me-7).

3.6. QM-NMR Calculational Section

For the QM-NMR calculations of compounds, conformational search was performed by using the torsional sampling (MCMM) approach and OPLS_2005 force field within an energy window of 21 kJ/mol. The following DFT calculations were performed using Gaussian 09, and Conformers above 1% population were reoptimized at the B3LYP/6-311G(d, p) level of theory. Magnetic shielding constants (σ) were calculated by means of the gauge including atomic orbitals (GIAO) method at the mPW1PW91/6-31+G(d, p) level of theory, as recommended for DP4+ analysis.

3.7. TDDFT-ECD Calculational Section

For the time-dependent density functional theory/electronic circular dichroism (TDDFT-ECD) calculations of compounds, conformational searches were performed following the general protocols previously described for QM-NMR calculation. Conformers above 1% population were used for re-optimizations and the following TDDFT-ECD calculations, which were performed using Gaussian 09 at the B3LYP/6-311G(d, p) level of theory with an IEFPCM solvent model for acetonitrile. Finally, the SpecDis 1.62 software was used to obtain the calculated ECD spectrum and visualize the results.

3.8. Antibacterial Assays

Five pathogenic bacteria, namely Streptococcus parauberis KSP28, Streptococcus parauberis SPOF3K, Lactococcus garvieae MP5245, Aeromonas salmonicida AS42, and Photobacterium damselae FP2244, were provided by the National Fisheries Research and Development Institute, Korea. The MIC values for all antimicrobial agents were measured by 96-well micro-dilution method. Mueller–Hinton II broth (cation-adjusted, BD 212322) was used for MIC value determination. Generally, compounds were dissolved with DMSO to 20 mM as stock solutions. All samples were diluted with culture broth to 500 μM as the initial concentration. Further 1:2 serial dilutions were performed through the addition of culture broth in order to reach concentrations ranging from 500 μM to 0.24 μM. A volume of 100 μL of each dilution was distributed in 96-well plates, as well as sterile controls, growth controls (containing culture broth plus DMSO, without compounds), and positive controls (containing culture broth plus positive control antibiotics). Each test and growth control wells were inoculated with 5 μL of an exponential-phase bacterial suspension (about 10⁵ CFU/well). The 96-well plates were incubated at 37 °C for 24 h. MIC vales of these compounds were defined as the lowest concentration needed to inhibit the bacterial growth completely. The units of the MIC values were transferred from “μM” to “μg/mL” according to compound’s molecular weight. All MIC values were interpreted according to recommendations of the Clinical and Laboratory Standards Institute (CLSI).

4. Conclusions

This detailed chemical investigation on the South China Sea soft coral K. molle yielded fifteen new diterpenes, namely xishaklyanes A-O (1–15), as well as three related known analogues (16–18). Among them, the absolute configuration of compound 18 was determined by the modified Mosher’s method. In addition, the stereochemistry of the new compounds was determined by QM-NMR and TDDFT-ECD, or chemical connections. In antibacterial activities on fish pathogenic bacteria, compound 4 exhibited the best activity, with an MIC of 0.225 μg/mL against Lactococcus garvieae. Further study should be conducted on the accumulation of the most effective compounds for in-depth antibacterial research.