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Article

Solid-Phase Extraction Embedded Dialysis (SPEED), an Innovative Procedure for the Investigation of Microbial Specialized Metabolites

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CNRS, Institut de Chimie des Substances Naturelles, UPR 2301, 1, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France
2
Équipe “Chimie des Substances Naturelles” BioCIS, CNRS, Université Paris-Saclay, 5 Rue J.-B. Clément, 92290 Châtenay-Malabry, France
3
PACIFIC BIOTECH SAS, BP 140 289, 98 701 Arue, Tahiti, French Polynesia
*
Author to whom correspondence should be addressed.
Academic Editors: Florbela Pereira and Susana P. Gaudencio
Mar. Drugs 2021, 19(7), 371; https://doi.org/10.3390/md19070371
Received: 15 June 2021 / Revised: 22 June 2021 / Accepted: 23 June 2021 / Published: 26 June 2021
(This article belongs to the Special Issue Marine Drug Discovery through Computer-Aided Approaches)
Solid-phase extraction embedded dialysis (SPEED technology) is an innovative procedure developed to physically separate in-situ, during the cultivation, the mycelium of filament forming microorganisms, such as actinomycetes and fungi, and the XAD-16 resin used to trap the secreted specialized metabolites. SPEED consists of an external nylon cloth and an internal dialysis tube containing the XAD resin. The dialysis barrier selects the molecular weight of the trapped compounds, and prevents the aggregation of biomass or macromolecules on the XAD beads. The external nylon promotes the formation of a microbial biofilm, making SPEED a biofilm supported cultivation process. SPEED technology was applied to the marine Streptomyces albidoflavus 19-S21, isolated from a core of a submerged Kopara sampled at 20 m from the border of a saltwater pond. The chemical space of this strain was investigated effectively using a dereplication strategy based on molecular networking and in-depth chemical analysis. The results highlight the impact of culture support on the molecular profile of Streptomyces albidoflavus 19-S21 secondary metabolites. View Full-Text
Keywords: solid-phase extraction SPE; XAD resin; molecular networking; Streptomyces; specialized metabolites; dereplication solid-phase extraction SPE; XAD resin; molecular networking; Streptomyces; specialized metabolites; dereplication
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MDPI and ACS Style

Mai, P.-Y.; Le Goff, G.; Poupon, E.; Lopes, P.; Moppert, X.; Costa, B.; Beniddir, M.A.; Ouazzani, J. Solid-Phase Extraction Embedded Dialysis (SPEED), an Innovative Procedure for the Investigation of Microbial Specialized Metabolites. Mar. Drugs 2021, 19, 371. https://doi.org/10.3390/md19070371

AMA Style

Mai P-Y, Le Goff G, Poupon E, Lopes P, Moppert X, Costa B, Beniddir MA, Ouazzani J. Solid-Phase Extraction Embedded Dialysis (SPEED), an Innovative Procedure for the Investigation of Microbial Specialized Metabolites. Marine Drugs. 2021; 19(7):371. https://doi.org/10.3390/md19070371

Chicago/Turabian Style

Mai, Phuong-Y., Géraldine Le Goff, Erwan Poupon, Philippe Lopes, Xavier Moppert, Bernard Costa, Mehdi A. Beniddir, and Jamal Ouazzani. 2021. "Solid-Phase Extraction Embedded Dialysis (SPEED), an Innovative Procedure for the Investigation of Microbial Specialized Metabolites" Marine Drugs 19, no. 7: 371. https://doi.org/10.3390/md19070371

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