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A Metabolomics Exploration of the Sexual Phase in the Marine Diatom Pseudo-nitzschia multistriata

1
Department of Chemical Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany
2
Integrative Marine Ecology Department, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, Italy
3
Center of Biomolecular Drug Research (BMWZ), Leibniz Universität, 30167 Hannover, Germany
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Mar. Drugs 2020, 18(6), 313; https://doi.org/10.3390/md18060313
Received: 26 May 2020 / Revised: 11 June 2020 / Accepted: 12 June 2020 / Published: 14 June 2020
Pseudo-nitzschia multistriata is a planktonic marine diatom with a diplontic life cycle comprising a short sexual phase, during which gametes are produced following the encounter of two diploid cells of opposite mating type (MT). Gene expression studies have highlighted the presence of substantial changes occurring at the onset of sexual reproduction. Herein, we have hypothesized that the amount and nature of cellular metabolites varies along the mating process. To capture the metabolome of Pseudo-nitzschia multistriata at different harvesting times in an unbiased manner, we undertook an untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry. Using three different extraction steps, the method revealed pronounced differences in the metabolic profiles between control cells in the vegetative phase (MT+ and MT−) and mixed strains of opposite MTs (cross) undergoing sexual reproduction. Of the 2408 high-quality features obtained, 70 known metabolites could be identified based on in-house libraries and online databases; additional 46 features could be classified by molecular networking of tandem mass spectra. The reduction of phytol detected in the cross can be linked to the general downregulation of photosynthesis during sexual reproduction observed elsewhere. Moreover, the role of highly regulated compounds such as 7-dehydrodesmosterol, whose changes in abundance were the highest in the experiment, oleamide, ectoine, or trigonelline is discussed. View Full-Text
Keywords: marine natural products; metabolomics; mass spectrometry; diatoms; Pseudo-nitzschia multistriata; sexual reproduction marine natural products; metabolomics; mass spectrometry; diatoms; Pseudo-nitzschia multistriata; sexual reproduction
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Fiorini, F.; Borgonuovo, C.; Ferrante, M.I.; Brönstrup, M. A Metabolomics Exploration of the Sexual Phase in the Marine Diatom Pseudo-nitzschia multistriata. Mar. Drugs 2020, 18, 313.

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