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Mar. Drugs 2017, 15(12), 376;

The Anti-Inflammatory Effect and Structure of EPCP1-2 from Crypthecodinium cohnii via Modulation of TLR4-NF-κB Pathways in LPS-Induced RAW 264.7 Cells

The Institute of Seawater Desalination and Multipurpose Utilization, SOA, Tianjin 300192, China
Authors to whom correspondence should be addressed.
Received: 3 November 2017 / Revised: 15 November 2017 / Accepted: 17 November 2017 / Published: 1 December 2017
(This article belongs to the Collection Marine Polysaccharides)
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Exopolysaccharide from Crypthecodinium cohnii (EPCP1-2) is a marine exopolysaccharide that evidences a variety of biological activities. We isolated a neutral polysaccharide from the fermentation liquid of Crypthecodinium cohnii (CP). In this study, a polysaccharide that is derived from Crypthecodinium cohnii were analyzed and its anti-inflammatory effect was evaluated on protein expression of toll-like receptor 4 and nuclear factor κB pathways in macrophages. The structural characteristics of EPCP1-2 were characterized by GC (gas chromatography) and GC-MS (gas Chromatography-Mass Spectrometer) analyses. The molecular weight was about 82.5 kDa. The main chain of EPCP1-2 consisted of (1→6)-linked mannopyranosyl, (1→6)-linked glucopyranosyl, branched-chain consisted of (1→3,6)-linked galactopyranosyl and terminal consisted of t-l-Rhapyranosyl. The in vitro anti-inflammatory activity was representated through assay of proliferation rate, pro-inflammatory factor (NO) and expressions of proteins on RAW 264.7, the macrophage cell line. The results revealed that EPCP1-2 exhibited significant anti-inflammatory activity by regulating the expression of toll-like receptor 4, mitogen-activated protein kinases, and Nuclear Factor-κB protein. View Full-Text
Keywords: Crypthecodinium cohnii; exo-polysaccharide; anti-inflammatory; TLR4-MAPKs/NF-κB pathways Crypthecodinium cohnii; exo-polysaccharide; anti-inflammatory; TLR4-MAPKs/NF-κB pathways

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Ma, X.; Xie, B.; Du, J.; Zhang, A.; Hao, J.; Wang, S.; Wang, J.; Cao, J. The Anti-Inflammatory Effect and Structure of EPCP1-2 from Crypthecodinium cohnii via Modulation of TLR4-NF-κB Pathways in LPS-Induced RAW 264.7 Cells. Mar. Drugs 2017, 15, 376.

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