Next Article in Journal
Dichotocejpins A–C: New Diketopiperazines from a Deep-Sea-Derived Fungus Dichotomomyces cejpii FS110
Previous Article in Journal
A Saponification Method for Chlorophyll Removal from Microalgae Biomass as Oil Feedstock
Article Menu

Export Article

Open AccessArticle
Mar. Drugs 2016, 14(9), 163;

The Brown Alga Stypopodium zonale (Dictyotaceae): A Potential Source of Anti-Leishmania Drugs

Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21.941-902, Brazil
Laboratório Produtos Naturais de Algas Marinhas (ALGAMAR), Departamento de Biologia Marinha, Instituto de Biologia, Universidade Federal Fluminense, Niterói 24.210-150, Brazil
Grupo de Produtos Naturais de Organismos Aquáticos (GPNOA), Núcleo em Ecologia e Desenvolvimento Sócioambiental de Macaé (NUPEM), Universidade Federal do Rio de Janeiro, Campus-Macaé, Rio de Janeiro 27.965-045, Brazil
Author to whom correspondence should be addressed.
Academic Editor: Keith B. Glaser
Received: 5 August 2016 / Revised: 31 August 2016 / Accepted: 1 September 2016 / Published: 8 September 2016
Full-Text   |   PDF [2230 KB, uploaded 8 September 2016]   |  


This study evaluated the anti-Leishmania amazonensis activity of a lipophilic extract from the brown alga Stypopodium zonale and atomaric acid, its major compound. Our initial results revealed high inhibitory activity for intracellular amastigotes in a dose-dependent manner and an IC50 of 0.27 μg/mL. Due to its high anti-Leishmania activity and low toxicity toward host cells, we fractionated the lipophilic extract. A major meroditerpene in this extract, atomaric acid, and its methyl ester derivative, which was obtained by a methylation procedure, were identified by nuclear magnetic resonance (NMR) spectroscopy. Both compounds inhibited intracellular amastigotes, with IC50 values of 20.2 μM (9 μg/mL) and 22.9 μM (10 μg/mL), and selectivity indexes of 8.4 μM and 11.5 μM. The leishmanicidal activity of both meroditerpenes was independent of nitric oxide (NO) production, but the generation of reactive oxygen species (ROS) may be at least partially responsible for the amastigote killing. Our results suggest that the lipophilic extract of S. zonale may represent an important source of compounds for the development of anti-Leishmania drugs. View Full-Text
Keywords: marine natural products; meroditerpenes; Stypopodium zonale; leshmanicidal activity marine natural products; meroditerpenes; Stypopodium zonale; leshmanicidal activity

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Soares, D.C.; Szlachta, M.M.; Teixeira, V.L.; Soares, A.R.; Saraiva, E.M. The Brown Alga Stypopodium zonale (Dictyotaceae): A Potential Source of Anti-Leishmania Drugs. Mar. Drugs 2016, 14, 163.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top