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Article

In Vitro Anticancer Activity of Phlorofucofuroeckol A via Upregulation of Activating Transcription Factor 3 against Human Colorectal Cancer Cells

1
Department of Bioresource Sciences, Andong National University, Andong 36729, Korea
2
Chuncheon Bioindustry Foundation, Chuncheon 24234, Korea
3
Department of Herbal Medicine Resource, Kangwon National University, Dogye 25949, Korea
4
Gyeongbuk Institute for Marine Bioindustry, Uljin 36315, Korea
*
Author to whom correspondence should be addressed.
These authors equally contributed to this study.
Academic Editor: Keith B. Glaser
Mar. Drugs 2016, 14(4), 69; https://doi.org/10.3390/md14040069
Received: 28 February 2016 / Revised: 18 March 2016 / Accepted: 24 March 2016 / Published: 29 March 2016
Phlorofucofuroeckol A (PFF-A), one of the phlorotannins found in brown algae, has been reported to exert anti-cancer property. However, the molecular mechanism for the anti-cancer effect of PFF-A has not been known. Activating transcription factor 3 (ATF3) has been reported to be associated with apoptosis in colorectal cancer. The present study was performed to investigate the molecular mechanism by which PFF-A stimulates ATF3 expression and apoptosis in human colorectal cancer cells. PFF-A decreased cell viability through apoptosis of human colorectal cancer cells. PFF-A increased ATF3 expression through regulating transcriptional activity. The responsible cis-element for ATF3 transcriptional activation by PFF-A was cAMP response element binding protein (CREB), located between positions −147 and −85 of the ATF3 promoter. Inhibition of p38, c-Jun N-terminal kinases (JNK), glycogen synthase kinase (GSK) 3β, and IκB kinase (IKK)-α blocked PFF-A-mediated ATF3 expression. ATF3 knockdown by ATF3 siRNA attenuated the cleavage of poly (ADP-ribose) polymerase (PARP) by PFF-A, while ATF3 overexpression increased PFF-A-mediated cleaved PARP. These results suggest that PFF-A may exert anti-cancer property through inducing apoptosis via the ATF3-mediated pathway in human colorectal cancer cells. View Full-Text
Keywords: activating transcription factor 3; apoptosis; human colorectal cancer; phlorofucofuroeckol A; Eisenia bicyclis activating transcription factor 3; apoptosis; human colorectal cancer; phlorofucofuroeckol A; Eisenia bicyclis
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MDPI and ACS Style

Eo, H.J.; Kwon, T.-H.; Park, G.H.; Song, H.M.; Lee, S.-J.; Park, N.-H.; Jeong, J.B. In Vitro Anticancer Activity of Phlorofucofuroeckol A via Upregulation of Activating Transcription Factor 3 against Human Colorectal Cancer Cells. Mar. Drugs 2016, 14, 69. https://doi.org/10.3390/md14040069

AMA Style

Eo HJ, Kwon T-H, Park GH, Song HM, Lee S-J, Park N-H, Jeong JB. In Vitro Anticancer Activity of Phlorofucofuroeckol A via Upregulation of Activating Transcription Factor 3 against Human Colorectal Cancer Cells. Marine Drugs. 2016; 14(4):69. https://doi.org/10.3390/md14040069

Chicago/Turabian Style

Eo, Hyun J., Tae-Hyung Kwon, Gwang H. Park, Hun M. Song, Su-Jin Lee, Nyun-Ho Park, and Jin B. Jeong 2016. "In Vitro Anticancer Activity of Phlorofucofuroeckol A via Upregulation of Activating Transcription Factor 3 against Human Colorectal Cancer Cells" Marine Drugs 14, no. 4: 69. https://doi.org/10.3390/md14040069

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