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Open AccessArticle

Biological Potential of Chitinolytic Marine Bacteria

1
Department of Biotechnology and Biomedicine, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark
2
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark
*
Author to whom correspondence should be addressed.
Academic Editor: Hitoshi Sashiwa
Mar. Drugs 2016, 14(12), 230; https://doi.org/10.3390/md14120230
Received: 18 November 2016 / Revised: 7 December 2016 / Accepted: 8 December 2016 / Published: 16 December 2016
(This article belongs to the Special Issue Advances in Marine Chitin and Chitosan II, 2017)
Chitinolytic microorganisms secrete a range of chitin modifying enzymes, which can be exploited for production of chitin derived products or as fungal or pest control agents. Here, we explored the potential of 11 marine bacteria (Pseudoalteromonadaceae, Vibrionaceae) for chitin degradation using in silico and phenotypic assays. Of 10 chitinolytic strains, three strains, Photobacterium galatheae S2753, Pseudoalteromonas piscicida S2040 and S2724, produced large clearing zones on chitin plates. All strains were antifungal, but against different fungal targets. One strain, Pseudoalteromonas piscicida S2040, had a pronounced antifungal activity against all seven fungal strains. There was no correlation between the number of chitin modifying enzymes as found by genome mining and the chitin degrading activity as measured by size of clearing zones on chitin agar. Based on in silico and in vitro analyses, we cloned and expressed two ChiA-like chitinases from the two most potent candidates to exemplify the industrial potential. View Full-Text
Keywords: chitin; chitinases; antifungal; marine bacteria; Pseudoalteromonadaceae; Vibrionaceae chitin; chitinases; antifungal; marine bacteria; Pseudoalteromonadaceae; Vibrionaceae
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MDPI and ACS Style

Paulsen, S.S.; Andersen, B.; Gram, L.; Machado, H. Biological Potential of Chitinolytic Marine Bacteria. Mar. Drugs 2016, 14, 230.

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