Chemical and Genetic Diversity of Nodularia spumigena from the Baltic Sea
Abstract
:1. Introduction
2. Results and Discussion
2.1. Non-Ribosomal Peptides and Peptide Profiles of Baltic Nodularia spumigena
2.2. PC-IGS Sequences and Phylogenetic Analyses of Baltic Nodularia spumigena
2.3. N. spumigena Chemotypes vs. Genotypes
3. Materials and Methods
3.1. Cultivation of Nodularia spumigena Strains
3.2. Extraction and LC-MS/MS Analysis of Peptides
3.3. DNA Extraction and PC-IGS Sequencing
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
AcSer | acetylserine |
Agm | agmatine |
Bu | butanoic acid |
Hex | hexanoic acid |
Hpla | hydroxyphenyl lactic acid |
Hph | homophenylalanine |
Hty | homotyrosine |
MePro | metylproline |
MetO | methionine sufloxide |
Met(O2) | methionine sulfone |
MeHty | methylhomotyrosine |
Oct | octanoic acid |
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Chemotype Cluster | N. spumigena Strain | Peptide m/z | Amino Acids Components | |||||
---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | |||
CT_A | CCNP1402, BY1, CCY9414, AV1, CCNP1427, CCNP1436, KAC7, KAC71 | 932 | Ile | Lys | MetO | Hph | MeHty | MetO |
916a | Ile | Lys | MetO | Hph | MeHty | Met | ||
914 | Ile | Lys | MetO | Hph | MeHty | AcSer | ||
900b | Ile | Lys | Met | Hph | MeHty | Met | ||
898a | Ile | Lys | Met | Hph | MeHty | AcSer | ||
898b | Ile | Lys | MetO | Hph | MeHph | AcSer | ||
884a | Ile | Lys | Met | Hph | MeHph | Met | ||
882a | Ile | Lys | Met | Hph | MeHph | AcSer | ||
882b | Ile | Lys | Ile | Hph | MeHty | Met | ||
880 | Ile | Lys | Ile | Hph | MeHty | AcSer | ||
872 | Ile | Lys | MetO | Hph | MeHty | Ser | ||
856a | Ile | Lys | Met | Hph | MeHty | Ser | ||
*840 | Ile | Lys | Met | Hph | MeHph | Ser | ||
CCNP1402, CCY9414, AV1, KAC71 | 930 | Ile | Lys | MetO2 | Hph | MeHty | AcSer | |
CCNP1402, BY1, CCY9414, CCNP1427, CCNP1436, KAC7, KAC71 | 856b | Ile | Lys | MetO | Hph | MeHph | Ser | |
CCNP1402, BY1, AV1, CCNP1427, CCNP1436, KAC7, KAC71 | *822 | Ile | Lys | Ile | Hph | MeHph | Ser | |
KAC71 | 824 | Ile | Lys | Val | Hph | MeHty | Ser | |
AV1 | 868 | Ile | Lys | Val | Hph | MeHty | Met | |
866 | Ile | Lys | Val | Hph | MeHty | AcSer | ||
*864 | Ile | Lys | Ile | Hph | MeHph | AcSer | ||
*850 | Ile | Lys | Val | Hph | MeHph | AcSer | ||
CT_B1 | KAC70, KAC87 | 884c | Ile | Lys | Val | Hph | MeHty | MetO |
868 | Ile | Lys | Val | Hph | MeHty | Met | ||
866 | Ile | Lys | Val | Hph | MeHty | AcSer | ||
*852 | Ile | Lys | Val | Hph | MeHph | Met | ||
824 | Ile | Lys | Val | Hph | MeHty | Ser | ||
*808a | Ile | Lys | Val | Hph | MeHph | Ser | ||
KAC87 | *850 | Ile | Lys | Val | Hph | MeHph | AcSer | |
CT_B2 | KAC 11 | 808b | Ile | Lys | Ile | Hty | MeAla | Phe |
794 | Ile | Lys | Val | Hty | MeAla | Phe | ||
CCNP1401, CCNP1403, CCNP1426, CCNP1430, KAC13 | 842 | Phe | Lys | Ile | Hty | MeAla | Phe | |
828 | Phe | Lys | Val | Hty | MeAla | Phe | ||
CT_B3 | CCNP1440, CCNP1431, CCNP1428, CCNP1423, CCNP1424, CCNP1425, KAC64, KAC66, KAC68 | 934 | Phe | Lys | Val | Hty | MeHty | MetO |
918 | Phe | Lys | Val | Hph | MeHty | MetO | ||
902 | Phe | Lys | Val | Hph | MeHty | Met | ||
900a | Phe | Lys | Val | Hph | MeHty | AcSer | ||
884b | Phe | Lys | Val | Hph | MeHph | AcSer | ||
CCNP1423, CCNP1424, CCNP1425, CCNP1428, CCNP1431, CCNP1440, KAC64, KAC66 | 916b | Phe | Lys | Val | Hty | MeHty | AcSer | |
CCNP1431, CCNP1440, KAC64, KAC66, KAC68 | *886 | Phe | Lys | Val | Hph | MeHph | Met | |
KAC66, KAC68 | 858 | Phe | Lys | Val | Hph | MeHTyr | Ser |
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Mazur-Marzec, H.; Bertos-Fortis, M.; Toruńska-Sitarz, A.; Fidor, A.; Legrand, C. Chemical and Genetic Diversity of Nodularia spumigena from the Baltic Sea. Mar. Drugs 2016, 14, 209. https://doi.org/10.3390/md14110209
Mazur-Marzec H, Bertos-Fortis M, Toruńska-Sitarz A, Fidor A, Legrand C. Chemical and Genetic Diversity of Nodularia spumigena from the Baltic Sea. Marine Drugs. 2016; 14(11):209. https://doi.org/10.3390/md14110209
Chicago/Turabian StyleMazur-Marzec, Hanna, Mireia Bertos-Fortis, Anna Toruńska-Sitarz, Anna Fidor, and Catherine Legrand. 2016. "Chemical and Genetic Diversity of Nodularia spumigena from the Baltic Sea" Marine Drugs 14, no. 11: 209. https://doi.org/10.3390/md14110209