Next Article in Journal
Marine Polysaccharides from Algae with Potential Biomedical Applications
Previous Article in Journal
Emerging Concepts Promising New Horizons for Marine Biodiscovery and Synthetic Biology
Article Menu

Export Article

Open AccessArticle

A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate

College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Peer Jacobson
Mar. Drugs 2015, 13(5), 2955-2966;
Received: 11 January 2015 / Revised: 28 April 2015 / Accepted: 5 May 2015 / Published: 13 May 2015
PDF [1448 KB, uploaded 13 May 2015]


APSL (active peptide from shark liver) is a hepatic stimulator cytokine from the liver of Chiloscyllium. It can effectively protect islet cells and improve complications in mice with alloxan-induced diabetes. Here, we demonstrate that the APSL sequence is present in the N-terminus of novel TBC (Tre-2, Bub2 and Cdc16) domain family, member 15 (TBC1D15) from Chiloscyllium plagiosum. This shark TBC1D15 gene, which contains an ORF of 2088 bp, was identified from a cDNA library of regenerating shark liver. Bioinformatic analysis showed that the gene is highly homologous to TBC1D15 genes from other species. Moreover, the N-terminus of shark TBC1D15 contains a motif of unknown function (DUF3548), which encompasses the APSL fragment. Rab-GAP activity analysis showed that shark TBC1D15 is a new member of the TBC1D15 family. These results demonstrated that shark TBC1D15 possesses Rab-GAP activity using Rab7 as a substrate, which is a common property of the TBC1D15 family. The involvement of APSL at the N-terminus of TBC1D15 also demonstrates that this protein might be involved in insulin signaling and may be associated with the development of type 2 diabetes. The current findings pave the way for further functional and clinical studies of these proteins from marine sources. View Full-Text
Keywords: APSL; TBC1D15 protein; Rab-GAP activity; type 2 diabetes APSL; TBC1D15 protein; Rab-GAP activity; type 2 diabetes

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Li, Y.; Wang, W.; Cheng, D.; Wang, T.; Lu, C.; Chen, J.; Nie, Z.; Zhang, W.; Lv, Z.; Wu, W.; Shu, J. A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate. Mar. Drugs 2015, 13, 2955-2966.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top