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Open AccessArticle

Lipid Peroxidation Is another Potential Mechanism besides Pore-Formation Underlying Hemolysis of Tentacle Extract from the Jellyfish Cyanea capillata

1
Department of Chemical Defense Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China
2
Department of Nephrology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
3
Department of Gynecology and Obstetrics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Mar. Drugs 2013, 11(1), 67-80; https://doi.org/10.3390/md11010067
Received: 22 September 2012 / Revised: 2 November 2012 / Accepted: 12 December 2012 / Published: 9 January 2013
(This article belongs to the Collection Bioactive Compounds from Marine Plankton)
This study was performed to explore other potential mechanisms underlying hemolysis in addition to pore-formation of tentacle extract (TE) from the jellyfish Cyanea capillata. A dose-dependent increase of hemolysis was observed in rat erythrocyte suspensions and the hemolytic activity of TE was enhanced in the presence of Ca2+, which was attenuated by Ca2+ channel blockers (Diltiazem, Verapamil and Nifedipine). Direct intracellular Ca2+ increase was observed after TE treatment by confocal laser scanning microscopy, and the Ca2+ increase could be depressed by Diltiazem. The osmotic protectant polyethylenglycol (PEG) significantly blocked hemolysis with a molecular mass exceeding 4000 Da. These results support a pore-forming mechanism of TE in the erythrocyte membrane, which is consistent with previous studies by us and other groups. The concentration of malondialdehyde (MDA), an important marker of lipid peroxidation, increased dose-dependently in rat erythrocytes after TE treatment, while in vitro hemolysis of TE was inhibited by the antioxidants ascorbic acid—Vitamin C (Vc)—and reduced glutathione (GSH). Furthermore, in vivo hemolysis and electrolyte change after TE administration could be partly recovered by Vc. These results indicate that lipid peroxidation is another potential mechanism besides pore-formation underlying the hemolysis of TE, and both Ca2+ channel blockers and antioxidants could be useful candidates against the hemolytic activity of jellyfish venoms. View Full-Text
Keywords: jellyfish; Cyanea capillata; hemolysis; pore-formation; lipid peroxidation jellyfish; Cyanea capillata; hemolysis; pore-formation; lipid peroxidation
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Wang, T.; Wen, X.-J.; Mei, X.-B.; Wang, Q.-Q.; He, Q.; Zheng, J.-M.; Zhao, J.; Xiao, L.; Zhang, L.-M. Lipid Peroxidation Is another Potential Mechanism besides Pore-Formation Underlying Hemolysis of Tentacle Extract from the Jellyfish Cyanea capillata. Mar. Drugs 2013, 11, 67-80.

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