Light-Regulated Gene Expression Patterns During Conidial Formation in Aspergillus oryzae
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Species
2.2. Culture Conditions
2.3. Light Conditions and Apparatus
2.4. RNA Extraction
2.5. Library Construction and Sequencing
2.6. Bioinformatics Analysis in RNA Sequencing
2.7. Relative Expression of Genes by qRT-PCR
2.8. Statistical Analyses of qRT-PCR
3. Results
3.1. Overall Regulation of Gene Expression by Light
3.2. Light-Regulated Genes Related to Conidial Formation
3.3. Light-Regulated Genes Among the Potential Photoreceptor-like Genes
3.4. Results of Genes in the MAPK Pathway
3.5. Light-Regulated Genes Were Sensitive to Light Dose
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Parameter | High Light Dose Treatment | Low Light Dose Treatment | ||||
---|---|---|---|---|---|---|
Light source | Blue | Green | Red | Blue | Green | Red |
Light wavelength (nm) | 475 | 520 | 630 | 475 | 520 | 630 |
Full width at half maximum | 25 | 37 | 21 | 25 | 37 | 21 |
Average irradiance (W·m–2) | 19.87 | 17.87 | 15.63 | 19.87 | 17.87 | 15.63 |
Irradiated time | 72 h | 30 min | ||||
Light dose (kJ·m–2) | 5150 | 4632 | 4051 | 35.8 | 32.2 | 28.1 |
Light intensity (μmol photon m−2 s−1) | 80 |
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Lin, S.; Yang, J.; Wang, A.; Fu, Q.; Huang, S.; Liu, M. Light-Regulated Gene Expression Patterns During Conidial Formation in Aspergillus oryzae. Curr. Issues Mol. Biol. 2025, 47, 373. https://doi.org/10.3390/cimb47050373
Lin S, Yang J, Wang A, Fu Q, Huang S, Liu M. Light-Regulated Gene Expression Patterns During Conidial Formation in Aspergillus oryzae. Current Issues in Molecular Biology. 2025; 47(5):373. https://doi.org/10.3390/cimb47050373
Chicago/Turabian StyleLin, Shangfei, Jiali Yang, Aixia Wang, Qiqi Fu, Shijie Huang, and Muqing Liu. 2025. "Light-Regulated Gene Expression Patterns During Conidial Formation in Aspergillus oryzae" Current Issues in Molecular Biology 47, no. 5: 373. https://doi.org/10.3390/cimb47050373
APA StyleLin, S., Yang, J., Wang, A., Fu, Q., Huang, S., & Liu, M. (2025). Light-Regulated Gene Expression Patterns During Conidial Formation in Aspergillus oryzae. Current Issues in Molecular Biology, 47(5), 373. https://doi.org/10.3390/cimb47050373