Deletion of Antigen-Presenting Cells in Lipopolysaccharide-Induced Acute Kidney Injury (AKI) Affects the Exacerbation and Repair in AKI
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Protocol in the Murine Model of LPS-Induced AKI
2.2. Assessment of Kidney Immunohistochemistry
2.3. Assessment of Renal mRNA Expression
2.4. Cytokine Production
2.5. FACS Analysis
2.6. Statistical Analysis
3. Results
3.1. The LC Treatment Depleted Renal Macrophages and Dendritic Subset in the Mice
3.2. Macrophage and DC Depletion Improved the Survival Rate by Inhibiting the Renal Injury after LPS-Induced AKI
3.3. LC Treatment Affected Renal Injury, the Infiltration of Inflammatory Cells, and Tubular Damage in the Mouse Kidney after LPS Injection
3.4. Serum Levels of Inflammatory Cytokines and the Intrarenal mRNA Expression of an Inflammatory Mediator after the LPS Injection
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
---|---|---|
18SrRNA | GTAACCCGTTGAACCCCATTC | GCCTCACTAAACCATCCAATCG |
IFN-γ | TGCTGATGGGAGGAGATGTCT | TTTCTTTCAGGGACAGCCTGTT |
TNF-α | CGATCACCCCGAAGTTCAGTA | GGTGCCTATGTCTCAGCCTCTT |
IL-10 | GGTTGCCAAGCCTTATCGGA | ACCTGCTCCACTGCCTTGCT |
CCL2/MCP-1 | AAAAACCTGGATCGGAACCAA | CGGGTCAACTTCACATTCAAAG |
ICAM-1 | CATCCCAGAGAAGCCTTCCTG | TCAGCCACTGAGTCTCCAAGC |
T-bet | CCTGGACCCAACTGTCAACT | AACTGTGTTCCCGAGGTGTC |
GATA3 | AGGGACATCCTGCGCGAACTGT | CATCTTCCGGTTTCGGGTCTGG |
Gene Name | TaqMan Gene Expression Assay ID |
---|---|
18SrRNA | 4310893E |
IL-6 | Mm00446190_m1 |
IL-12p40 | Mm00434174_m1 |
IL-18 | Mm00434226_m1 |
KIM-1 | Mm00506686_m1 |
18 h | 120 h | |
---|---|---|
LPS + LC vs. LPS Treated Group | ||
Cytokines | ||
IFN-γ | 14.7 ± 1.6 vs. 23.6 ± 3.0 * | 4.0 ± 0.6 vs. 3.0 ± 0.5 |
TNF | 24.9 ± 2.6 vs. 31.5 ± 1.2 * | 12.2 ± 3.3 vs. 8.0 ± 1.7 |
IL-6 | 1314.0 ± 190.7 vs. 3900.0 ± 580.8 **** | 42.6 ± 9.8 vs. 17.4 ± 3.3 * |
IL-10 | 56.2 ± 15.3 vs. 36.3 ± 11.1 | 5.8 ± 1.9 vs. 3.5 ± 0.5 |
IL-12p40 | 0.6 ± 0.1 vs. 0.6 ± 0.1 | 0.9 ± 0.2 vs. 1.0 ± 0.1 |
IL-18 | 5.9 ± 0.8 vs. 10.8 ± 0.9 ** | 1.8 ± 0.4 vs. 1.3 ± 0.1 |
Chemokines | ||
CCL2/MCP-1 | 75.0 ± 10.8 vs. 153.4 ± 18.5 ** | 13.4 ± 2.2 vs. 6.5 ± 1.5 * |
KIM-1 | 89.8 ± 16.0 vs. 258.7 ± 45.1 ** | 26.6 ± 16.8 vs. 2.7 ± 1.5 ** |
Th cell subset transcription factors | ||
T-bet | 1.7 ± 0.3 vs. 3.5 ± 0.6 * | 3.7 ± 1.0 vs. 5.2 ± 0.6 |
GATA3 | 0.9 ± 0.1 vs. 0.9 ± 0.1 | 0.9 ± 0.1 vs. 1.1 ± 0.1 |
Leukocyte adhesion molecule | ||
ICAM-1 | 61.3 ± 6.7 vs. 101.5 ± 16.7 * | 1.9 ± 0.3 vs. 1.9 ± 0.3 |
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Li, J.; Nozaki, Y.; Akazawa, H.; Kishimoto, K.; Kinoshita, K.; Matsumura, I. Deletion of Antigen-Presenting Cells in Lipopolysaccharide-Induced Acute Kidney Injury (AKI) Affects the Exacerbation and Repair in AKI. Curr. Issues Mol. Biol. 2022, 44, 5655-5665. https://doi.org/10.3390/cimb44110383
Li J, Nozaki Y, Akazawa H, Kishimoto K, Kinoshita K, Matsumura I. Deletion of Antigen-Presenting Cells in Lipopolysaccharide-Induced Acute Kidney Injury (AKI) Affects the Exacerbation and Repair in AKI. Current Issues in Molecular Biology. 2022; 44(11):5655-5665. https://doi.org/10.3390/cimb44110383
Chicago/Turabian StyleLi, Jinhai, Yuji Nozaki, Hiroki Akazawa, Kazuya Kishimoto, Koji Kinoshita, and Itaru Matsumura. 2022. "Deletion of Antigen-Presenting Cells in Lipopolysaccharide-Induced Acute Kidney Injury (AKI) Affects the Exacerbation and Repair in AKI" Current Issues in Molecular Biology 44, no. 11: 5655-5665. https://doi.org/10.3390/cimb44110383