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Volume 34
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10.21775/cimb.034.231
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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Baculovirus as Versatile Vectors for Protein Display and Biotechnological Applications

by
Chih-Hsuan Tsai
1,2,3,
Sung-Chan Wei
2,3,
Huei-Ru Lo
2 and
Yu-Chan Chao
1,2,3,4,5,*
1
Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan, China
2
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, China
3
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, China
4
Department of Plant Pathology and Microbiology, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan, China
5
Department of Life Sciences, College of Life Sciences, National Chung Hsing University, Taichung, Taiwan, China
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2019, 34(1), 231-256; https://doi.org/10.21775/cimb.034.231
Submission received: 2 March 2019 / Revised: 10 April 2019 / Accepted: 2 May 2019 / Published: 6 June 2019

Abstract

The baculovirus-insect cell system has long been deployed for a variety of applications including for use as biopesticides, for recombinant protein production, transient transgene expression, tissue therapy, and for vaccine production. Apart from the advantage of large-scale heterologous protein production with appropriate eukaryotic post-translational modification, foreign proteins can also be displayed on the viral envelope. This surface-display technology preserves the native multimeric structure of the protein, thereby expanding the clinical and pharmaceutical utility of the baculovirus system. Recombinant baculoviruses displaying major antigens for human or animal viruses can serve as appropriate vaccines. They can also serve as effective diagnostic platforms and various cell-based assay systems. In this review, we discuss progress in applying baculovirus surface-display, including protein display on the envelope, capsid, and occlusion bodies of baculoviruses, as well as on cells. We will also describe strategies for improvement of this biotechnological approach.

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MDPI and ACS Style

Tsai, C.-H.; Wei, S.-C.; Lo, H.-R.; Chao, Y.-C. Baculovirus as Versatile Vectors for Protein Display and Biotechnological Applications. Curr. Issues Mol. Biol. 2019, 34, 231-256. https://doi.org/10.21775/cimb.034.231

AMA Style

Tsai C-H, Wei S-C, Lo H-R, Chao Y-C. Baculovirus as Versatile Vectors for Protein Display and Biotechnological Applications. Current Issues in Molecular Biology. 2019; 34(1):231-256. https://doi.org/10.21775/cimb.034.231

Chicago/Turabian Style

Tsai, Chih-Hsuan, Sung-Chan Wei, Huei-Ru Lo, and Yu-Chan Chao. 2019. "Baculovirus as Versatile Vectors for Protein Display and Biotechnological Applications" Current Issues in Molecular Biology 34, no. 1: 231-256. https://doi.org/10.21775/cimb.034.231

APA Style

Tsai, C.-H., Wei, S.-C., Lo, H.-R., & Chao, Y.-C. (2019). Baculovirus as Versatile Vectors for Protein Display and Biotechnological Applications. Current Issues in Molecular Biology, 34(1), 231-256. https://doi.org/10.21775/cimb.034.231

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