Comparative Proteomic Analysis of tPVAT during Ang II Infusion

Abstract

Perivascular adipose tissue (PVAT) homeostasis plays an important role in maintaining vascular function, and PVAT dysfunction may induce several pathophysiological situations. In this study, we investigated the effect and mechanism of the local angiotensin II (Ang II) on PVAT. High-throughput comparative proteomic analysis, based on TMT labeling combined with LC-MS/MS, were performed on an in vivo Ang II infusion mice model to obtain a comprehensive view of the protein ensembles associated with thoracic PVAT (tPVAT) dysfunction induced by Ang II. In total, 5037 proteins were confidently identified, of which 4984 proteins were quantified. Compared with the saline group, 145 proteins were upregulated and 146 proteins were downregulated during Ang II-induced tPVAT pathogenesis. Bioinformatics analyses revealed that the most enriched GO terms were annotated as gene silencing, monosaccharide binding, and extracellular matrix. In addition, some novel proteins, potentially associated with Ang II infusion, were identified, such as acyl-CoA carboxylase α, very long-chain acyl-CoA synthetase (ACSVL), uncoupling protein 1 (UCP1), perilipin, RAS protein-specific guanine nucleotide-releasing factor 2 (RasGRF2), and hypoxia inducible factor 1α (HIF-1α). Ang II could directly participate in the regulation of lipid metabolism, transportation, and adipocyte differentiation by affecting UCP1 and perilipin. Importantly, the key KEGG pathways were involved in fatty acid biosynthesis, FABP3-PPARα/γ, RasGRF2-ERK-HIF-1α, RasGRF2-PKC-HIF-1α, and STAT3-HIF-1α axis. The present study provided the most comprehensive proteome profile of mice tPVAT and some novel insights into Ang II-mediated tPVAT dysfunction and will be helpful for understanding the possible relationship between local RAS activation and PVAT dysfunction.

1. Introduction

The renin–angiotensin–aldosterone system (RAAS) is involved in systemic blood pressure regulation and renal electrolyte homeostasis. It is well accepted that Ang II mediates vascular smooth muscle cell (VSMC) proliferation, migration, and vascular remodeling [1,2]. We also previously reported that Ang II regulates VSMC proliferation and vascular remodeling by RhoGDI signaling [3]. However, there is no detailed report on the effect of Ang II on PVAT (perivascular adipose tissue) structure and function.

PVAT is an adipose tissue with endocrine and paracrine functions surrounding the blood vessels, such as the small mesenteric and femoral arteries or the large abdominal aorta (AA), and plays wide-ranging physiological roles on vascular environment stability and influences vascular pathogenesis [4,5]. Under physiological conditions, PVAT has strong anti-atherosclerosis properties, induces non-shivering thermogenesis (NST), and metabolizes fatty acids by secreting various bioactive factors [6]. Under pathological conditions (such as obesity), PVAT loses its thermogenic ability and secretes pro-inflammatory adipocytokines that induce endothelial dysfunction and inflammatory cell infiltration and promotes the development of atherosclerosis [6]. The PVAT in the thoracic aorta is more similar to brown adipose tissue (BAT), while the PVAT in the abdominal aorta is similar to white adipose tissue (WAT). WAT primarily stores energy in the form of triglycerides, while excessive lipid accumulation in WAT leads to adipocyte hypertrophy and dysfunction. This leads to increased secretion of harmful fat factors and inflammatory cytokines into circulation, thus impairing the function of the vascular endothelium [7,8]. Unlike WAT, BAT can absorb lipids by uncoupling oxidation in the mitochondrial electron transport chain to generate heat, and this is characterized by the expression of the heat-producing gene uncoupling protein 1 (UCP1) [9]. In addition, WAT can be browned under the stimulation of low temperature and catecholamine [10,11]. The browning of WAT is characterized by a high expression of the brown adipocyte marker UCP1 [12], which is now called the third adipose tissue-beige adipose tissue (BEt). In general, changes in the phenotype of PVAT correlate with disease progression.

Many studies have shown that there is local RAAS activity in PVAT [13]. It has been reported that Ang II inhibits adipogenic differentiation of human preadipocytes in vitro via the AT1 receptor [14]. The AngII-AT2 or Ang (1–7)-Mas activation stimulates adipogenesis and antagonizes the antiadipogenic effect of AngII-AT1 activation [15,16]. However, the effect of Ang II on PVAT remains unclear. In this study, we used TMT, combined with LC-MS/MS and bioinformatics analysis, to analyze the phenotypic changes of PVAT during Ang II infusion.

2. Materials and Methods

2.1. Materials

Ang II was obtained from MedChemExpress (#HY-B0202; Princeton, NJ, USA). The primary antibodies against KDM1A (BM4356), ALCAM (A01788-1), MBNL1 (A02309-1), and TSN (A02777) were purchased from Boster Biological Technology (Wuhan, Hubei, China). Anti-TMEM41B (29270-1-AP), -PRTN3 (25278-1-AP), -GIGYF2 (24790-1-AP), -NHP2 (15128-1-AP), -RDH10 (14644-1-AP), and -TIMD4 (12008-1-AP) antibodies were purchased from Proteintech (Chicago, IL, USA). Anti-CtBP1 (ab129181), -PKN1 (ab231038), and -CCN1 (ab228592) antibodies were purchased from Abcam (Cambridge, MA, USA). Peroxidase-conjugated AffiniPure goat anti-rabbit IgG (H + L) was from Proteintech (SA00001–2; Chicago, IL, USA). An SABC immunohistochemistry staining kit was sourced from BosterBio (SA1028; Wuhan, Hubei, China). All other chemicals used in this study were analytical grade and were made in China.

2.2. Animals and Experimental Protocol

Animal procedures were performed in accordance with the Ethics Committee and the Animal Care and Use Committee of Nantong University (Ethic Committee approval number: 1213201.1) and conformed to the NIH Guide for the Care and Use of Laboratory Animals. Male C57BL/6 mice, aged 45 days weighing 20 ± 1 g (20 mice per group), were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). Mice were anesthetized by intraperitoneal injection of 3.6% chloral hydrate (11 mL/kg). For Ang II infusion, mice were implanted with an Alzet Model 1002 osmotic minipump (Alzet Corp, Cupertino, CA, USA) for subcutaneous infusion of Ang II at a rate of 1000 ng/kg/min [3]. Control mice were implanted with pumps for infusion of normal saline. Pumps were placed into the subcutaneous space of anesthetized mice through a small incision in the back of the neck. The incision was closed, and the mice were allowed to recover without medication. After 14 days, mice were then euthanized. The aortic tree was quickly separated and washed with pre-chilled saline for subsequent analysis.

2.3. Adipose Tissue Transplantation

The PVAT around the thoracic aorta was carefully removed with microforceps under a surgical microscope. The removed thoracic PVAT (tPVAT) was then weighed and placed in Dulbecco’s Modifed Eagle Medium (DMEM, Gibco, Termo Fisher Scientifc, Inc., Waltham, MA, USA) containing 1% antibiotics (R&D Systems, Inc., Minneapolis, MN, USA). For proteomics analysis, the normal and Ang II infusion group had no less than 600 mg of the removed tPVAT in each group. The remaining tPVAT and blood vessels were used for the following experiments or stored in a liquid nitrogen tank.

2.4. H&E Staining

The removed tPVAT around the thoracic aorta was fixed in 4% paraformaldehyde and then embedded in paraffin for staining with hematoxylin and eosin. For quantitative analysis of adipocyte diameter, tissue sections were captured with Olympus digital camera (Tokyo, Japan) and quantified via Image-Pro Plus 6.0 software (Media Cybernetics, Rockville, MD, USA).

2.5. Immunohistochemical Analysis

Immunohistochemical staining against KDM1A, ALCAM, MBNL1, TSN, TMEM41B, PRTN3, GIGYF2, NHP2, RDH10, TIMD4, CtBP1, PKN1, and CCN1 were performed using a strept avidin-biotin complex (SABC) immunohistochemistry staining kit and following the manufacturer’s instructions. Paraffin-embedded tissue sections of the transplanted tPVAT (5 μm thick) were deparafnized and blocked with 0.5% horse serum. The sections were then incubated with the primary antibodies overnight at 4 °C, followed by incubation with biotinylated anti-rabbit IgG as the secondary antibody at room temperature for 2 h and SABC for 1 h. The samples were then visualized using a diaminobenzidine (DAB) staining kit, followed by counterstaining with hematoxylin, in order to stain the target proteins brown. All images were captured using an Olympus digital camera (Olympus, Tokyo, Japan) and analyzed using the Image-Pro Plus 6.0 software program (Media Cybernetics, Rockville, MD, USA).

2.6. Quantitative Proteomic Profiling by Tandem Mass Tag (TMT) Technology

The flowchart of proteomics and bioinformatics analysis is shown in Figure 1.

The removed tPVAT around the thoracic aorta was ground to powder in liquid nitrogen. Proteins were extracted in lysis buffer (4% SDS, 100 mM Tris-HCL, 1 mM DTT, pH 7.6) for 30 min on ice. Then, cells were further broken using an ultrasonic cell disruptor, followed by centrifugation at 14,000 rpm for 1.5 h at 19 °C using a TL-100 ultracentrifuge (Beckman, Palo Alto, Brea, CA, USA). Finally, the middle layer of aqueous liquid was retained. The amount of protein was quantified with the BCA Protein Assay Kit (Bio-Rad, Hercules, CA, USA). Protein digestion by trypsin was performed according to filter-aided sample preparation (FASP) procedure described previously [17]. The digest peptides of each sample were desalted on C18 Cartridges (Empore™ SPE Cartridges C18, Oxford, PA, USA (standard density), bed I.D. 7 mm, volume 3 mL, Sigma), concentrated by vacuum centrifugation and reconstituted in 40 µL of 0.1% (v/v) formic acid. The peptide content was estimated by UV light spectral density at 280 nm using an extinctions coefficient of 1.1 of 0.1% (g/L) solution that was calculated on the basis of the frequency of tryptophan and tyrosine in vertebrate proteins.

100 μg peptide mixture of each sample was labeled using TMT reagent according to the manufacturer’s instructions (Thermo Scientific, Waltham, MA, USA). Labeled peptides were fractionated by SCX chromatography using the AKTA Purifier system (GE Healthcare, Chicago, IL, USA). The dried peptide mixture was reconstituted and acidified with buffer A (10 mM KH₂PO₄ in 25% of ACN, pH 3.0) and loaded onto a PolySULFOETHYL 4.6 × 100 mm column (5 µm, 200 Å, PolyLC Inc., Columbia, MD, USA). The peptides were eluted at a flow rate of 1 mL/min with a gradient of 0% buffer B (500 mM KCl, 10 mM KH₂PO₄ in 25% of ACN, pH 3.0) for 25 min, 0–10% buffer B during 25–32 min, 10–20% buffer B during 32–42 min, 20–45% buffer B during 42–47 min, 45–100% buffer B during 47–52 min, 100% buffer B during 52–60 min, and buffer B was reset to 0% after 60 min. The elution was monitored by absorbance at 214 nm, and fractions were collected every 1 min. The collected fractions were desalted on C18 Cartridges and concentrated by vacuum centrifugation.

Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific, Waltham, MA, USA) that was coupled to Easy nLC (Proxeon Biosystems, now Thermo Fisher Scientific) for 60/90 min. The peptides were loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18), connected to the C18-reversed phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin) in buffer A (0.1% Formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% Formic acid) at a flow rate of 300 nL/min, controlled by IntelliFlow technology. The mass spectrometer was operated in positive ion mode. MS data was acquired using a data-dependent top-10 method, dynamically choosing the most abundant precursor ions from the survey scan (300–1800 m/z) for HCD fragmentation. Automatic gain control (AGC) target was set to 3e6, and maximum inject time to 10 ms. Dynamic exclusion duration was 40.0 s. Survey scans were acquired at a resolution of 70,000 at m/z 200 and resolution for HCD spectra was set to 17,500 at m/z 200, and isolation width was 2 m/z. Normalized collision energy was 30 eV and the underfill ratio, which specifies the minimum percentage of the target value likely to be reached at maximum fill time, was defined as 0.1%. The instrument was run with peptide recognition mode enabled.

2.7. Identification and Quantitation of Proteins

The MS raw data for each sample were searched using the MASCOT engine (Matrix Science, London, UK; version 2.2), embedded into Proteome Discoverer 1.4 software for identification and quantitation analysis. Related parameters and instructions were shown in Supplementary Materials Table S1. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD029980.

2.8. Bioinformatic Analysis

Upregulated genes and downregulated genes were used to conduct a cluster analysis, a subcellular localization analysis, a Gene Ontology (GO, Valencia, Spain) analysis, a Kyoto Encyclopedia of Genes and Genomes (KEGG, Kyoto, Japan) pathway and enrichment analysis, and a protein-protein interaction (PPI, Hinxton, Cambridgeshire, UK) analysis.

2.8.1. Cluster Analysis

Cluster 3.0 (http://bonsai.hgc.jp/~mdehoon/software/cluster/software.htm, accessed on 17 February 2020) and Java Treeview software 3.0 (http://jtreeview.sourceforge.net, accessed on 17 February 2020) were used to perform hierarchical clustering analysis. Euclidean distance algorithm for similarity measure and average linkage clustering algorithm (clustering uses the centroids of the observations) for clustering were selected when performing hierarchical clustering. A heat map was often presented as a visual aid, in addition to the dendrogram.

2.8.2. Subcellular Localization

CELLO (http://cello.life.nctu.edu.tw/, accessed on 17 February 2020), which is a multi-class SVM classification system, was used to predict protein subcellular localization.

2.8.3. GO Analysis

The protein sequences of the selected differentially expressed proteins (DEPs) were locally searched using the NCBI BLAST+ client software (ncbi-blast-2.2.28 + -win32.exe) and InterProScan to find homologue sequences, then GO terms were mapped and sequences were annotated using the software program Blast2GO (version 2.5.0). The GO annotation results were plotted by R scripts.

2.8.4. KEGG Pathway

Following annotation steps, the studied proteins were blasted against the online KEGG database (http://geneontology.org/, accessed on 17 February 2020) to retrieve their KEGG orthology identifications and were subsequently mapped to pathways in KEGG. Enrichment analyses were applied based on the Fisher’ exact test, considering the whole quantified proteins as background dataset. Benjamini–Hochberg correction for multiple testing was further applied to adjust derived p-values. Only functional categories and pathways with p-values under a threshold of 0.05 were considered significant.

2.8.5. PPI Analysis

The PPI information of the studied proteins was retrieved from IntAct molecular interaction database (http://www.ebi.ac.uk/intact/, accessed on 17 February 2020) by their gene symbols or STRING software (http://string-db.org/, accessed on 17 February 2020, version 11.5). The results were downloaded in the XGMML format and imported into Cytoscape software (http://www.cytoscape.org/, accessed on 17 February 2020, version 3.2.1) to visualize and further analyze functional protein-protein interaction networks. Furthermore, the degree of each protein was calculated to evaluate the importance of the protein in the PPI network.

2.9. Statistical Analysis

All of the results are expressed as the mean ±SD. One-way ANOVA, followed by Tukey’s post-hoc test, as implemented in SPSS 22.0, was used for statistical analysis. Differences with a value of p < 0.05 were considered to be statistically significant.

3. Results

3.1. Pathological Conversion of tPVAT after Ang II Infusion

To determine the effects of Ang II on the tPVAT phenotype, male C57BL/6J mice aged 42–48 days were infused with Ang II or normal saline for two weeks. Mice that were infused with Ang II were significantly heavier than the controls by 7 and 12 days (Figure 2A). The tPVAT was successfully separated (Figure 2B,C). Figure 2C shows that Ang II infusion made the tPVAT morphology significantly irregular and caused hypertrophy, when compared with the saline group. The tPVAT weight of the Ang II infusion group was significantly higher than that of the control group (Figure 2D). Next, we histologically analyzed tPVAT after Ang II infusion. Mice infused with Ang II had a marked phenotypic conversion of tPVAT from a regular cell size to an irregular size (Figure 2E). Compared with the saline group, the Ang II-infused mice showed a substantial decrease in adipocyte size in tPVAT (Figure 2E).

3.2. Molecular Changes during tPVAT Pathogenesis Analyzed by LC-MS/MS

To elucidate the molecular events occurring in tPVAT during Ang II infusion, a quantitative proteomic analysis, based on TMT labeling, was executed in the in vivo Ang II infusion model. A total of 30,079 peptide fragments, of which 27,160 were unique peptides corresponding to a total of 5037 proteins (Figure S1), were used. We received a good quality deviation during the data acquisition process using a high-quality Q Exactive mass spectrometer. The mass deviations of all the identified peptides were primarily distributed within 10 ppm, indicating that the identification results were accurate and reliable (Figure S2A). We obtained an ideal score with a median of 27.49, and more than 65.64% of the peptides scored higher than 20 when evaluating each MS2 spectrogram (Figure S2B). The protein ratio distribution of the saline and the Ang II-infused group are shown in Figure S2C. A 1.2-fold change cut-off, with a p-value < 0.05, was used to indicate significant changes in the abundance of the differentially expressed proteins (DEPs) between the saline and Ang II-infused groups.

3.3. Identification of DEPs

In our study, the changes in expression of the DEPs between the saline and Ang II-infused groups were determined using a volcano plot and K-means clustering heatmaps as shown in Figure 3A,B. A total of 291 proteins were found to be significantly differentially expressed between the two groups, of which 145 proteins were upregulated and 146 proteins were downregulated (Figure 3C), indicating a drastic phenotypic alteration of tPVAT during Ang II infusion, when compared with the saline-infused group. The abbreviated lists of the up- and down-regulated proteins are provided in

Table 1. List of up-regulated proteins during Ang II infusion.

Accession NO.	Gene Name	Protein Name	Accession NO.	Gene Name	Protein Name
Q6ZQ88	KDM1A	Lysine-specific histone demethylase 1A	Q9CPV3	MRPL42	39S ribosomal protein L42, mitochondrial
P70268	PKN1	Serine/threonine-protein kinase N1	Q8VCW8	ACSF2	Medium-chain acyl-CoA ligase ACSF2, mitochondrial
Q8BX80	ENGase	Cytosolic endo-beta-N-acetylglucosaminidase	Q9D0F9	PGM1	Phosphoglucomutase-1
O88712	CtBP1	C-terminal-binding protein 1	P53986	SLC16A1	Monocarboxylate transporter 1
Q8K1A5	Tmem41b	Transmembrane protein 41B	Q8BGQ1	VIPAS39	Spermatogenesis-defective protein 39 homolog
P70392	RasGRF2	Ras-specific guanine nucleotide-releasing factor 2	Q9D2R0	AACS	Acetoacetyl-CoA synthetase
Q61096	PRTN3	Myeloblastin	Q9CXR1	DHRS7	Dehydrogenase/reductase SDR family member 7
Q8VD66	ABHD4	(Lyso)-N-acylphosphatidylethanolamine lipase	Q8K3K7	AGPAT2	1-acyl-sn-glycerol-3-phosphate acyltransferase beta
Q6Y7W8	GIGYF2	GRB10-interacting GYF protein 2	Q924N4	SLC12A6	Solute carrier family 12 member 6
Q61490	ALCAM	CD166 antigen	Q922Z0	DDO	D-aspartate oxidase
Q8CHD8	RAB11FIP3	Rab11-family interacting protein 3	Q14DH7	ACSS3	Acyl-CoA synthetase short-chain family member 3, mitochondrial
Q8R054	SRPX2	Sushi repeat-containing protein SRPX2	Q00898	SERPINA1E	Alpha-1-antitrypsin 1–5
Q3UVG3	FAM91A1	Protein FAM91A1	Q9JJF9	SPPL2a	Signal peptide peptidase-like 2A
O89017	LGMN	Legumain	Q9CS72	FILIP1	Filamin-A-interacting protein 1
Q8R1R3	StarD7	StAR-related lipid transfer protein 7, mitochondrial	Q9QXG4	ACSS2	Acetyl-coenzyme A synthetase, cytoplasmic
Q924W5	Smc6	Structural maintenance of chromosomes protein 6	P28063	PSMB8	Proteasome subunit beta type-8
Q64516	GK	Glycerol kinase	Q5RKZ7	MOCS1	Molybdenum cofactor biosynthesis protein 1
Q9D023	MPC2	Mitochondrial pyruvate carrier 2	P67984	Rpl22	60S ribosomal protein L22
E9Q1P8	IRF2BP2	Interferon regulatory factor 2-binding protein 2	Q99KF1	TMED9	Transmembrane emp24 domain-containing protein 9
P12242	UPC1	Mitochondrial brown fat uncoupling protein 1	Q9CY73	MRPL44	39S ribosomal protein L44, mitochondrial
P11404	FABP3	Fatty acid-binding protein, heart	Q8BHJ6	SERINC5	Serine incorporator 5
P09470	ACE	Angiotensin-converting enzyme	P70699	GAA	Lysosomal alpha-glucosidase
Q8BMB3	eIF4E2	Eukaryotic translation initiation factor 4E type 2	Q3U4G3	XXYLT1	Xyloside xylosyltransferase 1
Q9D136	OGFOD3	2-oxoglutarate and iron-dependent oxygenase domain-containing protein 3	Q8C7H1	MMAA	Methylmalonic aciduria type A homolog, mitochondrial
Q62009	POSTN	Periostin	Q80VW7	AKNA	Microtubule organization protein AKNA
Q78JN3	Eci3	Enoyl-CoA delta isomerase 3, peroxisomal	O54940	BNIP2	BCL2/adenovirus E1B 19 kDa protein-interacting protein 2
P68433	H3C1	Histone H3.1	Q6PEE2	CTIF	CBP80/20-dependent translation initiation factor
Q5SF07	IGF2BP2	Insulin-like growth factor 2 mRNA-binding protein 2	Q9D4H1	EXOC2	Exocyst complex component 2
Q9JM90	STAP1	Signal-transducing adaptor protein 1	Q8CFV9	RFK	Riboflavin kinase
Q80YX1	TNC	Tenascin	P29595	NEDD8	NEDD8
Q9DCE5	PAK1IP1	p21-activated protein kinase-interacting protein 1	Q9Z0P5	TWF2	Twinfilin-2
Q8JZR0	ACSL5	Long-chain-fatty-acid--CoA ligase 5	Q9CXJ1	EARS2	Probable glutamate--tRNA ligase, mitochondrial
Q9CXD9	LRRc17	Leucine-rich repeat-containing protein 17	Q8VHQ9	ACOT11	Acyl-coenzyme A thioesterase 11
Q8BWJ3	PHKA2	Phosphorylase b kinase regulatory subunit alpha, liver isoform	Q920B9	SUPT16H	FACT complex subunit SPT16
Q811L6	MAST4	Microtubule-associated serine/threonine-protein kinase 4	Q9DCV4	RMDN1	Regulator of microtubule dynamics protein 1
P61202	COPS2	COP9 signalosome complex subunit 2	P06745	GPI	Glucose-6-phosphate isomerase
Q1W617	SHROOM4	Protein SHROOM4	O08600	EndoG	Endonuclease G, mitochondrial
P63158	HMGB1	High mobility group protein B1	O08746	MATN2	Matrilin-2
P36536	SAR1A	GTP-binding protein SAR1A	Q78J03	MsrB2	Methionine-R-sulfoxide reductase B2, mitochondrial
Q8BJ03	COX15	Cytochrome c oxidase assembly protein COX15 homolog	Q99L45	EIF2S2	Eukaryotic translation initiation factor 2 subunit 2
Q8BHC0	LYVE-1	Lymphatic vessel endothelial hyaluronic acid receptor 1	P97452	BOP1	Ribosome biogenesis protein BOP1
Q8VE11	MTMR6	Myotubularin-related protein 6	O08528	HK2	Hexokinase-2
Q91V76	C11orf54	Ester hydrolase C11orf54 homolog	Q9Z1T1	Ap3B1	AP-3 complex subunit beta-1
Q64008	Rab34	Ras-related protein Rab-34	Q920A5	SCPEP1	Retinoid-inducible serine carboxypeptidase
P30993	C5aR1	C5a anaphylatoxin chemotactic receptor 1	Q91VU0	FAM3C	Protein FAM3C
Q91WC3	ACSL6	Long-chain-fatty-acid--CoA ligase 6	Q9CQE7	ERGIC3	Endoplasmic reticulum-Golgi intermediate compartment protein 3
Q7TPM3	TRIM17	E3 ubiquitin-protein ligase TRIM17	P50580	PA2G4	Proliferation-associated protein 2G4
P70122	SBDS	Ribosome maturation protein SBDS	Q9CZD5	MTIF3	Translation initiation factor IF-3, mitochondrial
Q8CHG3	GCC2	GRIP and coiled-coil domain-containing protein 2	P01631		Ig kappa chain V-II region 26–10
Q80X95	RRAGA	Ras-related GTP-binding protein A	Q9D125	MRPS25	28S ribosomal protein S25, mitochondrial
Q8R3F5	MCAT	Malonyl-CoA-acyl carrier protein transacylase, mitochondrial	Q5SW19	CLUH	Clustered mitochondria protein homolog
Q99LJ6	GPX7	Glutathione peroxidase 7	Q7TPE5	SLC7A6OS	Probable RNA polymerase II nuclear localization protein SLC7A6OS
O70503	HSD17B12	Very-long-chain 3-oxoacyl-CoA reductase	P42227	STAT3	Signal transducer and activator of transcription 3
P12382	PFKL	ATP-dependent 6-phosphofructokinase, liver type	Q9Z0S1	BPNT-1	3′(2′),5′-bisphosphate nucleotidase 1
Q61704	ITIH3	Inter-alpha-trypsin inhibitor heavy chain H3	P09242	ALPL	Alkaline phosphatase, tissue-nonspecific isozyme
P06797	CTSL	Cathepsin L1	P12265	GUSB	Beta-glucuronidase
Q80WG5	LRRC8A	Volume-regulated anion channel subunit LRRC8A	Q61391	MME	Neprilysin
Q9CQV4	RETREG3	Reticulophagy regulator 3	P82198	TGFBI	Transforming growth factor-beta-induced protein ig-h3
Q8BGR6	ARL15	ADP-ribosylation factor-like protein 15	Q9WUP7	UCHL5	Ubiquitin carboxyl-terminal hydrolase isozyme L5
P11276	FN1	Fibronectin	P52196	TST	Thiosulfate sulfurtransferase
P70460	VASP	Vasodilator-stimulated phosphoprotein	Q9QZA0	CA5B	Carbonic anhydrase 5B, mitochondrial
P06339	H2-T23	H-2 class I histocompatibility antigen, D-37 alpha chain	Q8QZY9	SF3B4	Splicing factor 3B subunit 4
Q65Z40	Wapl	Wings apart-like protein homolog	P34914	EPHX2	Bifunctional epoxide hydrolase 2
P10126	Eef1a1	Elongation factor 1-alpha 1	O54946	DNAJB6	DnaJ homolog subfamily B member 6
P54823	Ddx6	Probable ATP-dependent RNA helicase DDX6	Q9CQ86	MIEN1	Migration and invasion enhancer 1
Q8BUE4	Aifm2	Apoptosis-inducing factor 2	Q8CG46	SMC5	Structural maintenance of chromosomes protein 5
O35143	ATP5IF1	ATPase inhibitor, mitochondrial	Q60715	P4HA1	Prolyl 4-hydroxylase subunit alpha-1
Q9Z0V8	Timm17a	Mitochondrial import inner membrane translocase subunit Tim17-A	P15105	GLUL	Glutamine synthetase
Q9CWX9	Ddx47	Probable ATP-dependent RNA helicase DDX47	P08228	SOD1	Superoxide dismutase [Cu-Zn]
O35344	Kpna3	Importin subunit alpha-4	Q5SWU9	ACACA	Acetyl-CoA carboxylase 1
O54950	Prkag1	5’-AMP-activated protein kinase subunit gamma-1	Q91Z96	BMP2K	BMP-2-inducible protein kinase
Q99P65	Slc29a3	Equilibrative nucleoside transporter 3	Q9CYK1	WARS2	Tryptophan--tRNA ligase, mitochondrial
O09111	Ndufb11	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial

This table contains the 145 proteins that displayed more than 1.2-fold up-regulation in Ang II-infused mice in three independent experiments. The protein accession number, gene name, and name of each protein are provided here. The proteins are listed in descending order, according to their fold change (the fold change can be seen in Table S2), and the top 10 up-regulated proteins appear in bold.

and

Table 2. List of down-regulated proteins during Ang II infusion.

Accession NO.	Gene Name	Protein Name	Accession NO.	Gene Name	Protein Name
P18406	CCN1	CCN family member 1	A2RTL5	RSRC2	Arginine/serine-rich coiled-coil protein 2
Q09098	Pate4	Prostate and testis expressed protein 4	Q8BL86	MBLAC2	Metallo-beta-lactamase domain-containing protein 2
Q9ERR7	Selenof	Selenoprotein F	Q6ZWY8	TMSB10	Thymosin beta-10
O54916	Reps1	RalBP1-associated Eps domain-containing protein 1	Q9D5V6	Syap1	Synapse-associated protein 1
Q9CRB2	NHP2	H/ACA ribonucleoprotein complex subunit 2	Q8BK08	TMEM11	Transmembrane protein 11, mitochondrial
Q6U7R4	TIMD4	T-cell immunoglobulin and mucin domain-containing protein 4	Q80X80	C2CD2L	Phospholipid transfer protein C2CD2L
Q9JKP5	MBNL1	Muscleblind-like protein 1	Q9WV85	NME3	Nucleoside diphosphate kinase 3
Q62348	TSN	Translin	Q9DCL2	CIAO2A	Cytosolic iron-sulfur assembly component 2A
Q8VCH7	RDH10	Retinol dehydrogenase 10	Q61285	ABCD2	ATP-binding cassette sub-family D member 2
Q3THF9	COQ10B	Coenzyme Q-binding protein COQ10 homolog B, mitochondrial	P70333	HNRNPH2	Heterogeneous nuclear ribonucleoprotein H2
Q9JK38	GNPNAT1	Glucosamine 6-phosphate N-acetyltransferase	Q69ZP3	PNKD	Probable hydrolase PNKD
Q05144	Rac2	Ras-related C3 botulinum toxin substrate 2	O35864	COPS5	COP9 signalosome complex subunit 5
P60710	ACTB	Actin, cytoplasmic 1	Q61464	ZNF638	Zinc finger protein 638
Q9Z140	CPNE6	Copine-6	Q9JM14	NT5C	5’(3’)-deoxyribonucleotidase, cytosolic type
P47955	RPLP1	60S acidic ribosomal protein P1	Q9D1L0	CHCHD2	Coiled-coil-helix-coiled-coil-helix domain-containing protein 2
P63073	eIF4E	Eukaryotic translation initiation factor 4E	Q3TMW1	CCDC102A	Coiled-coil domain-containing protein 102A
Q8CH72	TRIM32	E3 ubiquitin-protein ligase TRIM32	Q9Z0V7	TIMM17B	Mitochondrial import inner membrane translocase subunit Tim17-B
P83870	PHF5A	PHD finger-like domain-containing protein 5A	O88895	HDAC3	Histone deacetylase 3
P41317	MBL2	Mannose-binding protein C	P97370	ATP1B3	Sodium/potassium-transporting ATPase subunit beta-3
P15864	H1-2	Histone H1.2	P26645	MARCKS	Myristoylated alanine-rich C-kinase substrate
Q91WU5	AS3MT	Arsenite methyltransferase	Q3TEA8	HP1BP3	Heterochromatin protein 1-binding protein 3
O54962	BANF1	Barrier-to-autointegration factor	Q9D1I2	CARD19	Caspase recruitment domain-containing protein 19
O70451	SLC16A7	Monocarboxylate transporter 2	Q3UEZ8	SLC10A4	Sodium/bile acid cotransporter 4
P26011	ITGB7	Integrin beta-7	Q99ME9	GTPBP4	Nucleolar GTP-binding protein 1
Q9JHH9	COPZ2	Coatomer subunit zeta-2	Q0VGM9	RTEL1	Regulator of telomere elongation helicase 1
O54788	DFFB	DNA fragmentation factor subunit beta	Q9JLQ0	CD2AP	CD2-associated protein
P82348	SGCG	Gamma-sarcoglycan	O70370	CTSS	Cathepsin S
B1AVZ0	UPRT	Uracil phosphoribosyltransferase homolog	Q69ZF3	GBA2	Non-lysosomal glucosylceramidase
Q9JHI7	EXOSC9	Exosome complex component RRP45	Q8K124	PLEKHO2	Pleckstrin homology domain-containing family O member 2
Q61207	PSAP	Prosaposin	P19973	LSP1	Lymphocyte-specific protein 1
O35638	STAG2	Cohesin subunit SA-2	P62874	GNB1	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
Q91YE5	BAZ2A	Bromodomain adjacent to zinc finger domain protein 2A	Q80VL1	TDRKH	Tudor and KH domain-containing protein
Q9CY57	Chtop	Chromatin target of PRMT1 protein	Q9Z2D6	MECP2	Methyl-CpG-binding protein 2
O55135	eIF6	Eukaryotic translation initiation factor 6	Q61503	NT5E	5’-nucleotidase
Q8R3B7	BRD8	Bromodomain-containing protein 8	Q8K2Q5	CHCHD7	Coiled-coil-helix-coiled-coil-helix domain-containing protein 7
Q8R143	PTTG1IP	Pituitary tumor-transforming gene 1 protein-interacting protein	Q8BP48	MetAP1	Methionine aminopeptidase 1
Q9D2V5	AAR2	Protein AAR2 homolog	Q64237	DBH	Dopamine beta-hydroxylase
P28798	GRN	Progranulin	O35226	PSMD4	26S proteasome non-ATPase regulatory subunit 4
Q9R0Y5	AK-1	Adenylate kinase isoenzyme 1	E9PZM4	CHD2	Chromodomain-helicase-DNA-binding protein 2
P03899	MTND3	NADH-ubiquinone oxidoreductase chain 3	P26369	U2AF2	Splicing factor U2AF 65 kDa subunit
Q8JZS0	LIN7A	Protein lin-7 homolog A	P56379	ATP5MPL	ATP synthase subunit ATP5MPL, mitochondrial
Q91V12	ACOT7	Cytosolic acyl coenzyme A thioester hydrolase	E9Q5C9	NOLC1	Nucleolar and coiled-body phosphoprotein 1
Q00724	RBP4	Retinol-binding protein 4	P59279	RAB2B	Ras-related protein Rab-2B
Q78HU7	GYPC	Glycophorin-C	Q8R332	NUP58	Nucleoporin p58/p45
Q9QZB0	RGS17	Regulator of G-protein signaling 17	P97384	ANXA11	Annexin A11
Q8BGX2	TIMM29	Mitochondrial import inner membrane translocase subunit Tim29	Q5XG73	ACBD5	Acyl-CoA-binding domain-containing protein 5
P97429	ANXA4	Annexin A4	Q60754	MARCO	Macrophage receptor MARCO
O55186	CD59a	CD59a glycoprotein	A2AJB2	TMEM141	Transmembrane protein 141
Q8BLV3	SLC9A7	Sodium/hydrogen exchanger 7	Q8K2I4	MANBA	Beta-mannosidase
Q0VBL1	TIGD2	Tigger transposable element-derived protein 2	P20444	PRKCA	Protein kinase C alpha type
O09117	SYPL1	Synaptophysin-like protein 1	P43276	H1.5	Histone H1.5
Q9WTR5	CDH13	Cadherin-13	Q8CGN5	PLIN1	Perilipin-1
Q8VCF0	MAVS	Mitochondrial antiviral-signaling protein	Q63844	MAPK3	Mitogen-activated protein kinase 3
O88492	PLIN4	Perilipin-4	Q3UMY5	EML4	Echinoderm microtubule-associated protein-like 4
Q80US4	ACTR5	Actin-related protein 5	Q6DIC0	SNF2L2	Probable global transcription activator SNF2L2
Q8C3X8	LMF2	Lipase maturation factor 2	P07356	ANXA2	Annexin A2
P99028	UQCRH	Cytochrome b-c1 complex subunit 6, mitochondrial	Q9WVH9	FBLN5	Fibulin-5
P58742	AAAS	Aladin	P43135	NR2F2	COUP transcription factor 2
Q6PCP5	Mff	Mitochondrial fission factor	Q8R4R6	NUP35	Nucleoporin NUP35
O35488	SLC27A2	Very long-chain acyl-CoA synthetase	P11798	CAMK2A	Calcium/calmodulin-dependent protein kinase type II subunit alpha
Q9DAS9	GNG12	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12	Q8BHL3	TBC1D10B	TBC1 domain family member 10B
Q9D1G5	LRRC57	Leucine-rich repeat-containing protein 57	Q8BTI8	SRRNM2	Serine/arginine repetitive matrix protein 2
Q8BGD8	COA6	Cytochrome c oxidase assembly factor 6 homolog	P97952	SCN1B	Sodium channel subunit beta-1
Q9CRB8	MTFP1	Mitochondrial fission process protein 1	P51912	SLC1A5	Neutral amino acid transporter B (0)
Q99MS7	EHBP1L1	EH domain-binding protein 1-like protein 1	Q8BPB5	EFEMP1	EGF-containing fibulin-like extracellular matrix protein 1
Q61029	TMPO	Lamina-associated polypeptide 2, isoforms beta/delta/epsilon/gamma	Q64373	BCL2L1	Bcl-2-like protein 1
Q91YU6	LZTS2	Leucine zipper putative tumor suppressor 2	Q6P9Q4	FHOD1	FH1/FH2 domain-containing protein 1
Q8K212	PACS1	Phosphofurin acidic cluster sorting protein 1	Q99L88	SNTB1	Beta-1-syntrophin
Q3U3R4	LMF1	Lipase maturation factor 1	Q80UZ0	FGD5	FYVE, RhoGEF and PH domain-containing protein 5
Q9JI46	NUDT3	Diphosphoinositol polyphosphate phosphohydrolase 1	O35704	SPTLC1	Serine palmitoyltransferase 1
Q8K4G1	LTBP-4	Latent-transforming growth factor beta-binding protein 4	A2ASQ1	AGRN	Agrin
P70429	EVL	Ena/VASP-like protein	Q9JIG8	PRAF2	PRA1 family protein 2
C0HKD8	MFAP1a	Microfibrillar-associated protein 1A	Q8R323	RFC3	Replication factor C subunit 3

This table contains the 146 proteins that displayed less than 0.83-fold down-regulation in Ang II-infused mice in three independent experiments. The protein accession number, gene name, and name of each protein are provided here. The proteins are listed in ascending order according to their fold change (the fold change can be seen in Table S2), and the top 10 down-regulated proteins appear in bold.

. As demonstrated in Table 1, most of the top 10 up-regulated proteins have been shown to be involved in epigenetic modification, cell phenotype changes, and differentiation. These proteins include KDM1A, PKN1, and ENGase [18,19,20]. In addition, CtBP1 participates in BAT differentiation [21], and TMEM41B is a novel ER-localized regulator of lipid mobilization and lipid droplets [22]. Regarding the down-regulated proteins, as demonstrated in Table 2, many of the top 10 proteins have been shown to participate in lipid metabolism, brown adipogenesis, and various pathological processes, such as coronary heart disease (CHD) and robust adiposity [23,24,25,26,27,28].

In addition, in order to verify the results of the TMT proteomics, we selected the top 10 up- or down-regulated proteins for immunohistochemical analyses. Figure 4 shows that the expressions of KDM1A, PKN1, CtBP1, TMEM41B, PRTN3, GIGYF2, and ALCAM were significantly up-regulated, while that of CCN1, MBNL1, NHP2, RDH10, TIMD4, and TSN were significantly decreased after Ang II infusion. The immunohistochemical results confirmed the expression pattern observed in the quantitative proteomics analysis. Since there were no suitable antibodies for ENGase, RasGRF2, ABHD4, Pate4, Selenof, Reps1, and COQ10B, we did not perform immunohistochemical verification of these proteins.

3.4. Bioinformatics Analysis of the Altered Proteins during Ang II Infusion

3.4.1. GO Functional Annotation and Analysis

We used the subcellular structure prediction software CELLO to analyze the subcellular location of all the DEPs. As shown in Figure 5A, most of the proteins were distributed in the nucleus, cytoplasm, extracellular matrix, and mitochondria. The DEPs were then analyzed against the GO database using three sets of ontologies: biological process (BP), molecular function (MF), and cellular component (CC). The most enriched GO terms of BP, MF, and CC were annotated as a gene silencing (12 proteins, richFactor = 0.16), monosaccharide binding (9 proteins, richFactor = 0.17), extracellular matrix (22 proteins, richFactor = 0.12), respectively (Figure 5B,C and

Table 3. Distribution of proteins and signaling pathways response to Ang II infusion, based on GO and KEGG analysis.

Terms	Count	p Value	FDR	richFactor	Accession NO.
GO (gene ontology)
Gene silencing (BP)	12	0.0011	0.5010	0.1600	Q91YE5, Q9Z2D6, O88712, Q6ZQ88, Q6Y7W8, Q9CY73, Q80VL1, P15864, P43276, O55135, P42227, P68433
Monosaccharide binding (MF)	9	0.0029	0.5010	0.1698	P41317, Q8K2I4, O08528, Q60715, Q64237, Q9D136, P12382, P06745, Q9JK38
Extracellular matrix (CC)	22	0.0012	0.5010	0.1164	P07356, P11276, Q9WVH9, P97429, Q8K4G1, Q62009, P97384, A2ASQ1, C0HKD8, P06797, P41317, Q8BPB5, Q80YX1, O08746, P82198, P18406, Q61704, Q8R054, Q60754, O70503, P09242, Q9CXD9
KEGG (kyoto encyclopedia of genes and genomes) pathways
Fatty acid biosynthesis	4	0.0069	0.5894	0.2857	Q5SWU9, Q8JZR0, Q8R3F5, Q91WC3
EGFR tyrosine kinase inhibitor resistance	6	0.0076	0.5894	0.1935	Q63844, Q8BMB3, P63073, Q64373, P20444, P42227
PPAR signaling pathway	8	0.0092	0.5894	0.1538	P12242, Q8CGN5, O88492, Q8JZR0, P11404, Q64516, O35488, Q91WC3
HIF-1 signaling pathway	8	0.0103	0.5894	0.1509	P12382, O08528, P11798, Q63844, Q8BMB3, P63073, P20444, P42227
Galactose metabolism	4	0.0114	0.5894	0.2500	P12382, Q9D0F9, O08528, P70699
Starch and sucrose metabolism	4	0.0143	0.6137	0.2353	Q9D0F9, O08528, P06745, P70699
Other glycan degradation	3	0.0169	0.6230	0.3000	Q8BX80, Q69ZF3, Q8K2I4
Peroxisome	8	0.0210	0.6757	0.1333	P08228, Q8JZR0, P34914, Q78JN3, Q61285, O35488, Q91WC3, Q922Z0
Lysosome	8	0.0323	0.7758	0.1231	Q61207, Q9Z1T1, P06797, O89017, P12265, P70699, O70370, Q8K2I4
Thiamine metabolism	2	0.0427	0.7758	0.3333	P09242, Q9R0Y5
Apoptosis	7	0.0443	0.7758	0.1228	P60710, O08600, P06797, Q63844, Q64373, O70370, O54788
Ras signaling pathway	8	0.0472	0.7758	0.1143	P62874, Q05144, Q9DAS9, Q63844, Q64373, P20444, P70392, Q9D4H1

Abbreviations: BP, biological processes; MF, molecular functions; CC, cellular components; FDR, false discovery rate. GO and KEGG pathway enrichment were analyzed by the Fisher’ exact test, based on the entire quantified protein annotations as the background dataset. Only functional categories and pathways with p-values < 0.05 were considered as significant.

). Other important BPs were included in DNA packaging, monocarboxylic acid transport, positive regulation of immunoglobulin secretion, organic acid transmembrane transport, carboxylic acid transmembrane transport, etc. (Figure 5C). Other important MFs included CoA-ligase activity, acid-thiol ligase activity, medium-chain fatty acid-CoA ligase activity, ligase activity, forming carbon-sulfur bonds, and organic hydroxy compound transmembrane transporter activity (Figure 5C). Other important CCs included host intracellular organelles, host intracellular membrane-bounded organelles, SUMO ligase complex, and Smc5–Smc6 complex (Figure 5C).

3.4.2. KEGG Pathway Analysis

By searching the major biological pathways and relevant regulatory processes involved in the KEGG, we analyzed all of the DEPs in the saline and Ang II infusion groups. The results of the KEGG pathway analysis showed that the significant enrichment pathways included fatty acid (FA) biosynthesis (4 proteins, richFactor = 0.29), EGFR tyrosine kinase inhibitor resistance (6 proteins, richFactor = 0.19), PPAR signaling pathway (8 proteins, richFactor = 0.15), HIF-1 signaling pathway (8 proteins, richFactor = 0.15), galactose metabolism (4 proteins, richFactor = 0.25), starch and sucrose metabolism (4 proteins, richFactor = 0.24), other glycan degradation (3 proteins, richFactor = 0.30), peroxisome (8 proteins, richFactor = 0.13), lysosome (8 proteins, richFactor = 0.12), thiamine metabolism (2 proteins, richFactor = 0.33), apoptosis (7 proteins, richFactor = 0.12), and Ras signaling pathway (8 proteins, richFactor = 0.11) (Figure 5D and Table 3).

Next, we connected these significant enrichment pathways and found that Ang II infusion deeply affected the fat metabolism and adipocyte differentiation (Figure 6A,B). Figure 6A shows that the up-regulation of acetyl-CoA carboxylase α and acyl-CoA synthetase long-chain family member 5 significantly affected the synthesis of long-chain FAs. In addition, Figure 6B shows that Ang II infusion affected the PPAR signaling pathway by regulating very long-chain acyl-CoA synthase (ACSVL) and FA binding protein 3 (FABP3), and thereby affected lipid metabolism, thermogenesis, and adipocyte differentiation. Additionally, we also found that Ang II infusion could affect many downstream signaling pathways, such as Rac, PI3K/Akt, MAPK, and the HIF-1α pathways, by regulating the Ras signaling that, in turn, affected many cell functions and participated in pathogenesis (Figure 6C).

3.4.3. PPI Analysis

In the PPI network, there were 20 high-connectivity degree DEPs with a large circle and a degree value of more than 10 between the saline and Ang II groups (Figure 7). These high-connectivity DEPs were identified as fibronectin (P11276), actin (P60710), mitogen-activated protein kinase (MAPK) 3 (Q63844), H/ACA ribonucleoprotein complex subunit 2 (Q9CRB2), signal transducer and activator of transcription (STAT) 3 (P42227), proliferation-associated protein 2G4 (P50580), nucleolar GTP-binding protein 1 (Q99ME9), eukaryotic translation initiation factor 6 (O55135), COP9 signalosome complex subunit 2 (P61202), Bcl-2-like protein 1 (Q64373), ACSVL (O35488), acetyl-CoA carboxylase 1 (Q5SWU9), 60S ribosomal protein L22 (P67984), hexokinase-2 (O08528), superoxide dismutase [Cu-Zn] (P08228), eukaryotic translation initiation factor 4E (P63073), high mobility group protein B1 (P63158), eukaryotic translation initiation factor 2 subunit 2 (Q99L45), lysine-specific histone demethylase 1A (Q6ZQ88), and ubiquitin carboxyl-terminal hydrolase isozyme L5 (Q9WUP7) (

Table 4. DEPs with high connectivity degree in PPI analysis between saline and Ang II infusion group.

Accession NO.	Gene Name	Protein Name	Degree	Up/Down Regulation
P11276	FN1	Fibronectin	28	up
P60710	ACTB	Actin, cytoplasmic 1	25	down
Q63844	MAPK3	Mitogen-activated protein kinase 3	17	down
Q9CRB2	NHP2	H/ACA ribonucleoprotein complex subunit 2	17	down
P42227	STAT3	Signal transducer and activator of transcription 3	16	up
P50580	PA2G4	Proliferation-associated protein 2G4	15	up
Q99ME9	GTPBP4	Nucleolar GTP-binding protein 1	14	down
O55135	eIF6	Eukaryotic translation initiation factor 6	14	down
P61202	COPS2	COP9 signalosome complex subunit 2	12	up
Q64373	BCL2L1	Bcl-2-like protein 1	11	down
O35488	SLC27A2	Very long-chain acyl-CoA synthetase	11	down
Q5SWU9	ACACA	Acetyl-CoA carboxylase 1	11	up
P67984	Rpl22	60S ribosomal protein L22	11	up
O08528	HK2	Hexokinase-2	10	up
P08228	SOD1	Superoxide dismutase [Cu-Zn]	10	up
P63073	eIF4E	Eukaryotic translation initiation factor 4E	10	down
P63158	HMGB1	High mobility group protein B1	10	up
Q99L45	EIF2S2	Eukaryotic translation initiation factor 2 subunit 2	10	up
Q6ZQ88	KDM1A	Lysine-specific histone demethylase 1A	10	up
Q9WUP7	UCHL5	Ubiquitin carboxyl-terminal hydrolase isozyme L5	10	up

). Some high-connectivity node proteins in Table 4 were related to the MAPK-Erk, STAT, FA biosynthesis, PPAR, and apoptosis signaling pathways, and these were highly consistent with those obtained using KEGG.

4. Discussion

PVAT is the connective tissue surrounding most of the systemic blood vessels and is now considered to be an important endocrine tissue that maintains the vascular homeostasis. Healthy PVAT has anti-contraction, anti-inflammation, and anti-oxidation effects [6]. According to the region of the vascular layer, where PVAT is located, PVAT shows phenotypic and functional heterogeneity [29]. For example, in the aorta of rodents, PVAT is primarily composed of WAT in the abdominal region, while PVAT is primarily composed of BAT in the thoracic region [30]. Angiotensinogen (AGT) was first found in BAT around the aorta in 1987 [31]. Evidence has demonstrated that the local renin-angiotensin system (RAS) may play an important role in adipocyte growth and differentiation [32]. However, the effect of pathological local RAS activation on the normal physiological function of PVAT is still unknown. Because Ang II is the primary component of RAS, we used the mice Ang II infusion model in the present study to simulate the pathological increase of local RAS at tPVAT. The results showed that Ang II infusion significantly increased the body weight and tPVAT weight of mice. In addition, the morphology of tPVAT also changed, and the distribution of tPVAT around the thoracic aorta appeared uneven (Figure 2A–D). To further verify the influence of Ang II infusion on the structure of tPVAT, we performed H&E staining on tPVAT tissue. The result showed that Ang II significantly changed the diameter and size of adipocytes in tPVAT (Figure 2E). These results indicate that the pathological activation of local RAS may greatly change the structure and function of tPVAT, and thereby affect vascular function.

To further clarify the molecular mechanisms of tPVAT structural changes caused by Ang II infusion, TMT label-based nanoscale LC–MS/MS was used in the present study. We identified a number of novel proteins associated with Ang II infusion and extended our understanding of this process. A total of 291 with significantly differential expression (145 up-regulation and 146 down-regulation) were identified in three biological replicates, and the hierarchical cluster analysis showed that these proteins were wel-distinguished. This indicated that the overall protein screening had reasonable accuracy. Additionally, we selected the top 10 differential expressed proteins with appropriate antibodies for immunohistochemical verification, and the data showed trends that paralleled those observed in the TMT results (Figure 4). To our knowledge, this is the first and most comprehensive large-scale proteomic profiling of Ang II-infused tPVAT, and the data will facilitate future studies of pathological tPVAT after the local activation of RAS.

The GO enrichment results indicated that the most enriched BP, MF, and CC categories after Ang II infusion were gene silencing (up regulation: CtBP1, KDM1A, GIGYF2, MRPL44, STAT3, H3C1; down regulation: BAZ2A, MECP2, TDRKH, H1-2, H1.5, eIF6), monosaccharide binding (up regulation: HK2, P4HA1, OGFOD3, PFKL, GPI; down regulation: Mbl2, MANBA, DBH, GNPNAT1), and extracellular matrix (up regulation: FN1, POSTN, CTSL, TNC, MATN2, TGFBI, ITIH3, SRPX2, HSD17B12, ALPL, LRRc17; down regulation: ANXA2, FBLN5, ANXA4, LTBP-4, ANXA11, AGRN, MFAP1a, Mbl2, EFEMP1, CCN1, Marco), respectively. We found that the genes in gene silencing, such as CtBP1, KDM1A, MECP2, STAT3, eIF6, were involved in regulating adipocyte inflammatory, differentiation, reconstruction, oxidation, and metabolism [21,33,34,35,36,37,38]. In addition, a large number of extracellular matrix components and regulatory proteins have changed after Ang II infusion, and these may participate in regulating cell proliferation, migration, and other phenotypic transformations.

As highlighted by the KEGG analysis, the most important networks, after Ang II infusion, were related to FA biosynthesis, PPAR signaling, Ras signaling, MAPK-Erk pathway, STAT3, and the HIF-1α pathway (Figure 6). Although it has been reported that Ang II induces adipocyte dysfunction, the mechanisms of this action are not very clear [39]. Our current study found that Ang II affected the synthesis of long-chain FAs, by up-regulating acyl-CoA carboxylase α and down-regulating ACSVL (Figure 6A,B). Additionally, these two FA synthesis-related enzymes had a high connectivity degree in the PPI analysis (Figure 7, Table 4) and could serve as possible novel molecular targets for Ang II action. Furthermore, Ang II affected the PPARγ signaling by up-regulating FABP3, which, in turn, promoted UCP1 and suppressed perilipin1, 4 expressions (Figure 6B). FABP3 is one of the members of the lipid-binding proteins superfamily. It is both membrane-bound, aiding cellular long-chain FA uptake, and cytoplasmic, being crucial to intracellular transport of FAs to sites of metabolic conversion [40]. It has been reported that the FABP3-PPARα axis has an indispensable role in Ang II-induced cardiac hypertrophy and heart failure [41]. Therefore, we speculate that the FABP3-PPARα axis also plays an important role in Ang II-induced tPVAT pathogenesis. In addition, Ang II promoted UCP1 and inhibited perilipin 4 expressions (Figure 6B). UCP1 primarily exists in BAT and is a mitochondrial protein embedded in the inner membrane of mitochondria, and it can dissipate the energy stored in mitochondrial electrochemical gradient as heat and “decouple” from ATP synthesis [42]. The thermogenesis of BAT and the energy steady state of the system depend entirely on the action of UCP1, and UCP1 up-regulation indicates adipocyte browning [43,44]. Therefore, the pathological increase of Ang II in this study may affect tPVAT thermometabolism and preadipocyte browning by up-regulating UCP1, which in turn affects vascular function. Perilipin proteins were discovered in the adipocyte, where they regulate lipid storage and lipolysis and are considered direct PPARγ targets [45,46]. Perilipin 1 appears to be a highly specific marker for adipocytic differentiation [47]. Perilipin 4 participates in the formation of lipid droplets and is an adipocyte regulator of triglyceride synthesis and packaging [48]. In the current study, Ang II may regulate the differentiation and function of adipocytes at mice tPVAT by down-regulating perilipin 1 and 4. In brief, the present study suggests that UCP1 and perilipin may be new targets of Ang II infusion, allowing Ang II to directly participate in the regulation of lipid metabolism, transportation, and adipocyte differentiation.

The Ras-ERK pathway is largely known for its regulation of cell proliferation, differentiation, survival, and drug-mediated behaviors. A recent study found that RasGRF2 (RAS protein-specific guanine nucleotide-releasing factor 2) mediates cocaine self-administration (SA) in mice via an ERK-dependent mechanism [49]. Our study discovered that RasGRF2 was up-regulated after Ang II infusion and could serve as a novel target for Ang II action, and the RasGRF2-Ras-ERK signal axis may play an important role in Ang II-mediated tPVAT pathogenesis. In addition, hypoxia inducible factor 1α (HIF-1α) has been reported to participate in the Ang II-mediated inflammatory response process [50], cardiomyocyte hypertrophy [51], preautonomic neurons activation [52], and vascular remodeling [53]. Our study showed a decreased expression level of HIF-1α after Ang II infusion and indicated that HIF-1α may be also a novel biomarker for Ang II-mediated tPVAT pathogenesis. In addition, RasGRF2-ERK-HIF-1α, RasGRF2-PKC-HIF-1α, and the STAT3-HIF-1α axis may be three important pathways at tPVAT with locally pathological activation of RAS, which can be further studied by subsequent researchers. A recent report showed that STAT3/HIF-1α signaling participates in peritoneal fibrosis during long-term peritoneal dialysis (PD) treatment [54]. Our results indicated an increased expression of STAT3 after Ang II infusion, and STAT3 had a high connectivity degree in the PPI analysis (Figure 6C and Table 4). Thus, STAT3 signaling may play an important role in Ang II-mediated phenotypic changes of tPVAT.

In conclusion, the present quantitative proteomic study provided the most comprehensive proteome profiles of mice tPVAT and a list of DEPs between saline-infused and Ang II-infused tPVAT. The bioinformatics analyses found that the functions of these altered proteins were primarily concentrated in gene silencing and the extracellular matrix. More importantly, the present study provided novel molecular targets of Ang II, such as acyl-CoA carboxylase α, ACSVL, UCP1, perilipin, and RasGRF2, as well as important signaling pathways, such as FA biosynthesis, FABP3-PPARα/γ, RasGRF2-ERK-HIF-1α, RasGRF2-PKC-HIF-1α, and STAT3-HIF-1α, during Ang II-induced tPVAT pathogenesis. The data provided here will promote our understanding of the effects of local RAS activation on PVAT and vascular function.