Antimicrobial activity and cytotoxicity of fifty one extracts of different parts of 14 plants were studied. Ethanol, methanol, aqueous, butanol, and
n-hexane extracts were tested against three Gram negative, two Gram positive bacteria, and two fungi. Cytotoxicity and phytochemical screening were determined
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Antimicrobial activity and cytotoxicity of fifty one extracts of different parts of 14 plants were studied. Ethanol, methanol, aqueous, butanol, and
n-hexane extracts were tested against three Gram negative, two Gram positive bacteria, and two fungi. Cytotoxicity and phytochemical screening were determined using MTT and TLC assays, respectively. Of the fifty one extracts, twenty two showed activities against different microorganisms with MICs ranging from 62.5 to 1000 µg/mL. The highest activity (100% inhibition) was for a butanol extract of
Rosa damascena receptacles against
Salmonella typhimurium and
Bacillus cereus (MIC of 62.5 and 250 µg/mL) respectively. Butanol extract of
Narcissus tazetta aerial parts and aqueous extract of
Rosa damascena receptacles were both active against
Candida albicans (MIC of 125 µg/mL). Methicillin-resistant
Staphylococcus aureus was inhibited by butanol, aqueous extracts of
Rosa damascena receptacles and butanol extract of
Inula viscosa flowers (MIC of 500, 500, and 250 µg/mL) respectively.
Rosa damascena receptacles and
Verbascum sinaiticum flowers ethanol extract showed lowest cytoxicity against Vero cell line (IC
50 of 454.11and 367.11). Most toxic was the ethanol extract of
Ononis hirta aerial parts (IC
50 72.50 µg/mL). Flavonoids and terpenoids were present in all plants.
Ononis hirta and
Narcissus tazetta contained alkaloids. The results validate the use of these plants and report for the first time bioactivity of
Rosa damascena receptacles and further justifies the use of such screening programs in the quest for new drugs.
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