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Molecules 2010, 15(3), 1632-1644; doi:10.3390/molecules15031632
Article

The Isolated and Combined Effects of Folic Acid and Synthetic Bioactive Compounds against Aβ(25-35)-Induced Toxicity in Human Microglial Cells

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Received: 18 February 2010; in revised form: 10 March 2010 / Accepted: 10 March 2010 / Published: 11 March 2010
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Abstract: Folic acid plays an important role in neuronal development. A series of newly synthesized bioactive compounds (NSCs) was reported to exhibit immunoactive and neuroprotective functions. The isolated and combined effects of folic acid and NSCs against β-amyloid (Aβ)-induced cytotoxicity are poorly understood. These effects were tested using human microglia cells (C13NJ) subjected to Aβ(25-35) challenge. According to an MTT assay, treatment of C13NJ cells with Aβ(25-35) at 10~100 μM for 48 h induced 18%~43% cellular death in a dose-dependent manner (p < 0.05). Aβ(25-35) treatment at 25 μM induced nitrite oxide (NO) release, elevated superoxide production, and reduced the distribution of cells in the S phase. Preincubation of C13NJ with 100 μM folic acid protected against Aβ(25-35)-induced cell death, which coincided with a reduction in NO release by folic acid supplements. NSC47 at a level of 50 μM protected against Aβ(25-35)-induced cell death and reduced Aβ-promoted superoxide production (p < 0.05). Folic acid in combination with NSC47 at their cytoprotective doses did not synergistically ameliorate Aβ(25-35)-associated NO release, superoxide production, or cell cycle arrest. Taken together, folic acid or NSC treatment alone, but not the combined regimen, protected against Aβ(25-35)-induced cell death, which may partially, if not completely, be mediated by free radical-scavenging effects.
Keywords: folate; Aβ(25-35) peptide; microglial cells; newly synthesized compounds; superoxide production; nitric oxide folate; (25-35) peptide; microglial cells; newly synthesized compounds; superoxide production; nitric oxide
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Liew, Y.-F.; Huang, C.-T.; Chou, S.-S.; Kuo, Y.-C.; Chou, S.-H.; Leu, J.-Y.; Tzeng, W.-F.; Wang, S.-J.; Tang, M.-C.; Huang, R.-F. The Isolated and Combined Effects of Folic Acid and Synthetic Bioactive Compounds against Aβ(25-35)-Induced Toxicity in Human Microglial Cells. Molecules 2010, 15, 1632-1644.

AMA Style

Liew Y-F, Huang C-T, Chou S-S, Kuo Y-C, Chou S-H, Leu J-Y, Tzeng W-F, Wang S-J, Tang M-C, Huang R-F. The Isolated and Combined Effects of Folic Acid and Synthetic Bioactive Compounds against Aβ(25-35)-Induced Toxicity in Human Microglial Cells. Molecules. 2010; 15(3):1632-1644.

Chicago/Turabian Style

Liew, Yih-Fong; Huang, Chao-Tzu; Chou, Shang-Shing P.; Kuo, Yuh-Chi; Chou, Shiu-Huey; Leu, Jyh-Yih; Tzeng, Woan-Fang; Wang, Su-Jane; Tang, Ming-Chi; Huang, Rwei-Fen Syu. 2010. "The Isolated and Combined Effects of Folic Acid and Synthetic Bioactive Compounds against Aβ(25-35)-Induced Toxicity in Human Microglial Cells." Molecules 15, no. 3: 1632-1644.


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