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Molecules 2010, 15(3), 1632-1644; doi:10.3390/molecules15031632
Article

The Isolated and Combined Effects of Folic Acid and Synthetic Bioactive Compounds against Aβ(25-35)-Induced Toxicity in Human Microglial Cells

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1 Department of Nutritional Science, Fu-Jen University, 510 Chung-Cheng Rd., Hsinchuang, Taipei County 242, Taiwan 2 Department of Chemistry, Fu-Jen University, 510 Chung-Cheng Rd., Hsinchuang, Taipei County 242, Taiwan 3 Department of Life Science, Fu-Jen University, 510 Chung-Cheng Rd., Hsinchuang, Taipei County 242, Taiwan 4 School of Medicine, Fu-Jen University, 510 Chung-Cheng Rd., Hsinchuang, Taipei County 242, Taiwan
* Author to whom correspondence should be addressed.
Received: 18 February 2010 / Revised: 10 March 2010 / Accepted: 10 March 2010 / Published: 11 March 2010
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Abstract

Folic acid plays an important role in neuronal development. A series of newly synthesized bioactive compounds (NSCs) was reported to exhibit immunoactive and neuroprotective functions. The isolated and combined effects of folic acid and NSCs against β-amyloid (Aβ)-induced cytotoxicity are poorly understood. These effects were tested using human microglia cells (C13NJ) subjected to Aβ(25-35) challenge. According to an MTT assay, treatment of C13NJ cells with Aβ(25-35) at 10~100 μM for 48 h induced 18%~43% cellular death in a dose-dependent manner (p < 0.05). Aβ(25-35) treatment at 25 μM induced nitrite oxide (NO) release, elevated superoxide production, and reduced the distribution of cells in the S phase. Preincubation of C13NJ with 100 μM folic acid protected against Aβ(25-35)-induced cell death, which coincided with a reduction in NO release by folic acid supplements. NSC47 at a level of 50 μM protected against Aβ(25-35)-induced cell death and reduced Aβ-promoted superoxide production (p < 0.05). Folic acid in combination with NSC47 at their cytoprotective doses did not synergistically ameliorate Aβ(25-35)-associated NO release, superoxide production, or cell cycle arrest. Taken together, folic acid or NSC treatment alone, but not the combined regimen, protected against Aβ(25-35)-induced cell death, which may partially, if not completely, be mediated by free radical-scavenging effects.
Keywords: folate; Aβ(25-35) peptide; microglial cells; newly synthesized compounds; superoxide production; nitric oxide folate; (25-35) peptide; microglial cells; newly synthesized compounds; superoxide production; nitric oxide
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Liew, Y.-F.; Huang, C.-T.; Chou, S.-S.; Kuo, Y.-C.; Chou, S.-H.; Leu, J.-Y.; Tzeng, W.-F.; Wang, S.-J.; Tang, M.-C.; Huang, R.-F. The Isolated and Combined Effects of Folic Acid and Synthetic Bioactive Compounds against Aβ(25-35)-Induced Toxicity in Human Microglial Cells. Molecules 2010, 15, 1632-1644.

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