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Molecules 2010, 15(3), 2000-2015; doi:10.3390/molecules15032000

The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells

Chair and Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 4 February 2010 / Revised: 9 March 2010 / Accepted: 19 March 2010 / Published: 22 March 2010
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Isoflavones are a class of bioactive polyphenols with cancer chemopreventive properties. TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) is a naturally occurring antitumor agent that selectively induces programmed death (apoptosis) in cancer cells. Polyphenols can modulate TRAIL-mediated apoptosis in cancer cells. We examined the cytotoxic and apoptotic activities of isoflavones in combination with TRAIL on HeLa cancer cells. The apoptosis was detected by fluorescence microscopy with annexin V-FITC. The cytotoxicity was evaluated by MTT and LDH assays. The tested isoflavones: genistein, biochanin-A and neobavaisoflavone enhance TRAIL-induced apoptosis in HeLa cells. Our study indicated that isoflavones augmented TRAIL-cytotoxicity against cancer cells and confirmed potential role of those polyphenols in chemoprevention.
Keywords: isoflavones; TRAIL; apoptosis; chemoprevention; cancer cells isoflavones; TRAIL; apoptosis; chemoprevention; cancer cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Bronikowska, J.; Szliszka, E.; Czuba, Z.P.; Zwolinski, D.; Szmydki, D.; Krol, W. The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells. Molecules 2010, 15, 2000-2015.

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