Next Article in Journal
Antioxidant Capacities and Phenolic Levels of Different Varieties of Serbian White Wines
Previous Article in Journal
QSAR Models for Reproductive Toxicity and Endocrine Disruption Activity
Molecules 2010, 15(3), 2000-2015; doi:10.3390/molecules15032000

The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells

Chair and Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 4 February 2010 / Revised: 9 March 2010 / Accepted: 19 March 2010 / Published: 22 March 2010
Download PDF [204 KB, uploaded 18 June 2014]


Isoflavones are a class of bioactive polyphenols with cancer chemopreventive properties. TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) is a naturally occurring antitumor agent that selectively induces programmed death (apoptosis) in cancer cells. Polyphenols can modulate TRAIL-mediated apoptosis in cancer cells. We examined the cytotoxic and apoptotic activities of isoflavones in combination with TRAIL on HeLa cancer cells. The apoptosis was detected by fluorescence microscopy with annexin V-FITC. The cytotoxicity was evaluated by MTT and LDH assays. The tested isoflavones: genistein, biochanin-A and neobavaisoflavone enhance TRAIL-induced apoptosis in HeLa cells. Our study indicated that isoflavones augmented TRAIL-cytotoxicity against cancer cells and confirmed potential role of those polyphenols in chemoprevention.
Keywords: isoflavones; TRAIL; apoptosis; chemoprevention; cancer cells isoflavones; TRAIL; apoptosis; chemoprevention; cancer cells
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Bronikowska, J.; Szliszka, E.; Czuba, Z.P.; Zwolinski, D.; Szmydki, D.; Krol, W. The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells. Molecules 2010, 15, 2000-2015.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert