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The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells
Chair and Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 4 February 2010; in revised form: 9 March 2010 / Accepted: 19 March 2010 / Published: 22 March 2010
Abstract: Isoflavones are a class of bioactive polyphenols with cancer chemopreventive properties. TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) is a naturally occurring antitumor agent that selectively induces programmed death (apoptosis) in cancer cells. Polyphenols can modulate TRAIL-mediated apoptosis in cancer cells. We examined the cytotoxic and apoptotic activities of isoflavones in combination with TRAIL on HeLa cancer cells. The apoptosis was detected by fluorescence microscopy with annexin V-FITC. The cytotoxicity was evaluated by MTT and LDH assays. The tested isoflavones: genistein, biochanin-A and neobavaisoflavone enhance TRAIL-induced apoptosis in HeLa cells. Our study indicated that isoflavones augmented TRAIL-cytotoxicity against cancer cells and confirmed potential role of those polyphenols in chemoprevention.
Keywords: isoflavones; TRAIL; apoptosis; chemoprevention; cancer cells
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Bronikowska, J.; Szliszka, E.; Czuba, Z.P.; Zwolinski, D.; Szmydki, D.; Krol, W. The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells. Molecules 2010, 15, 2000-2015.
Bronikowska J, Szliszka E, Czuba ZP, Zwolinski D, Szmydki D, Krol W. The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells. Molecules. 2010; 15(3):2000-2015.
Bronikowska, Joanna; Szliszka, Ewelina; Czuba, Zenon P.; Zwolinski, Dariusz; Szmydki, Dariusz; Krol, Wojciech. 2010. "The Combination of TRAIL and Isoflavones Enhances Apoptosis in Cancer Cells." Molecules 15, no. 3: 2000-2015.