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Molecules 2010, 15(3), 1690-1704; doi:10.3390/molecules15031690
Article

HTS-Driven Discovery of New Chemotypes with West Nile Virus Inhibitory Activity

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Received: 25 January 2010 / Revised: 20 February 2010 / Accepted: 8 March 2010 / Published: 12 March 2010
(This article belongs to the Special Issue High-throughput Screening)
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Abstract

West Nile virus (WNV) is a positive sense, single-stranded RNA virus that can cause illness in humans when transmitted via mosquito vectors. Unfortunately, no antivirals or vaccines are currently available, and therefore efficient and safe antivirals are urgently needed. We developed a high throughput screen to discover small molecule probes that inhibit virus infection of Vero E6 cells. A primary screen of a 13,001 compound library at a 10 µM final concentration was conducted using the 384-well format. Z′ values ranged from 0.54–0.83 with a median of 0.74. Average S/B was 17 and S/N for each plate ranged from 10.8 to 23.9. Twenty-six compounds showed a dose response in the HT screen and were further evaluated in a time of addition assay and in a titer reduction assay. Seven compounds showed potential as small molecule probes directed at WNV. The hit rate from the primary screen was 0.185% (24 compounds out of 13,001 compounds) and from the secondary screens was 0.053% (7 out of 13,001 compounds) respectively.
Keywords: West Nile virus; high throughput screen; antivirals; chemotypes West Nile virus; high throughput screen; antivirals; chemotypes
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chung, D.H.; Jonsson, C.B.; Maddox, C.; McKellip, S.N.; Moore, B.P.; Heil, M.; White, E.L.; Ananthan, S.; Li, Q.; Feng, S.; Rasmussen, L. HTS-Driven Discovery of New Chemotypes with West Nile Virus Inhibitory Activity. Molecules 2010, 15, 1690-1704.

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