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Molecules 2010, 15(3), 1645-1667; doi:10.3390/molecules15031645

Hitherto Unrecognized Fluorescence Properties of Coniferyl Alcohol

1,2,* , 1,3
1 Joint BioEnergy Institute (JBEI), Emeryville, CA 94550, USA 2 Biosensors and Nanomaterials Department, Sandia National Laboratories, Albuquerque, NM 87185, USA 3 Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA 4 Sandia National Laboratories, Livermore, CA 94550, USA
* Author to whom correspondence should be addressed.
Received: 14 January 2010 / Revised: 23 February 2010 / Accepted: 8 March 2010 / Published: 11 March 2010
(This article belongs to the Special Issue High-throughput Screening)
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We instituted a quasi-quality assurance program for demonstrating coniferyl alcohol’s fluorescence and fluorescence diminishment following enzymatic oxidation. The magnitude of diminishment was a measure of catalysis. High throughput screening was performed in pseudo-kinetic and endpoint modes by measuring the fluorescence at 416 nm following excitation at 290, 310 or 340 nm. Dose-response tracings were linear between two and three orders of magnitude with average limits of detection and quantitation of 1.8 and 6.9 mM coniferyl alcohol, respectively. Oxidation was evident with 0.025 mg/mL laccase or 0.003 mg/mL peroxidase or inside 5 min using 0.5 mg/mL laccase or 5 mM substrate. Sodium chloride inhibited (IC50, 25 mM) laccase oxidation of coniferyl alcohol. Fluorescence from 10 concentrations (1 to 1000 mM) of coniferyl alcohol was stable for 24 hours over 14 excitation/emission cycles at 3 different combinations of excitation and emission wavelengths. In conclusion, coniferyl alcohol absorption and fluorescence assays should facilitate biomass lignin analyses and improve delignification.
Keywords: coniferyl alcohol; fluorescence; laccase; peroxidase; high throughput screening; lignin; biofuels coniferyl alcohol; fluorescence; laccase; peroxidase; high throughput screening; lignin; biofuels
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Achyuthan, K.E.; Adams, P.D.; Datta, S.; Simmons, B.A.; Singh, A.K. Hitherto Unrecognized Fluorescence Properties of Coniferyl Alcohol. Molecules 2010, 15, 1645-1667.

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