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Hitherto Unrecognized Fluorescence Properties of Coniferyl Alcohol
Joint BioEnergy Institute (JBEI), Emeryville, CA 94550, USA
Biosensors and Nanomaterials Department, Sandia National Laboratories, Albuquerque, NM 87185, USA
Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
Sandia National Laboratories, Livermore, CA 94550, USA
* Author to whom correspondence should be addressed.
Received: 14 January 2010; in revised form: 23 February 2010 / Accepted: 8 March 2010 / Published: 11 March 2010
Abstract: We instituted a quasi-quality assurance program for demonstrating coniferyl alcohol’s fluorescence and fluorescence diminishment following enzymatic oxidation. The magnitude of diminishment was a measure of catalysis. High throughput screening was performed in pseudo-kinetic and endpoint modes by measuring the fluorescence at 416 nm following excitation at 290, 310 or 340 nm. Dose-response tracings were linear between two and three orders of magnitude with average limits of detection and quantitation of 1.8 and 6.9 mM coniferyl alcohol, respectively. Oxidation was evident with 0.025 mg/mL laccase or 0.003 mg/mL peroxidase or inside 5 min using 0.5 mg/mL laccase or 5 mM substrate. Sodium chloride inhibited (IC50, 25 mM) laccase oxidation of coniferyl alcohol. Fluorescence from 10 concentrations (1 to 1000 mM) of coniferyl alcohol was stable for 24 hours over 14 excitation/emission cycles at 3 different combinations of excitation and emission wavelengths. In conclusion, coniferyl alcohol absorption and fluorescence assays should facilitate biomass lignin analyses and improve delignification.
Keywords: coniferyl alcohol; fluorescence; laccase; peroxidase; high throughput screening; lignin; biofuels
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Achyuthan, K.E.; Adams, P.D.; Datta, S.; Simmons, B.A.; Singh, A.K. Hitherto Unrecognized Fluorescence Properties of Coniferyl Alcohol. Molecules 2010, 15, 1645-1667.
Achyuthan KE, Adams PD, Datta S, Simmons BA, Singh AK. Hitherto Unrecognized Fluorescence Properties of Coniferyl Alcohol. Molecules. 2010; 15(3):1645-1667.
Achyuthan, Komandoor Elayavalli; Adams, Paul David; Datta, Supratim; Simmons, Blake Alexander; Singh, Anup Kumar. 2010. "Hitherto Unrecognized Fluorescence Properties of Coniferyl Alcohol." Molecules 15, no. 3: 1645-1667.