One new phenylpropanoid, turformosin A (
1), and one new triterpene, turformosinic acid (
2), together with 16 known compounds, were isolated from the stems of
Turpinia formosana Nakai. All structures were elucidated on the basis of spectroscopic analysis, including 1D-
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One new phenylpropanoid, turformosin A (
1), and one new triterpene, turformosinic acid (
2), together with 16 known compounds, were isolated from the stems of
Turpinia formosana Nakai. All structures were elucidated on the basis of spectroscopic analysis, including 1D- and 2D-NMR techniques and MS analysis. Selected isolated compounds were evaluated for
in vitro cytotoxicity against four human cancer cell lines and antioxidant scavenging effects on DPPH. (−)-(7′
S,8′
S)-
threo-carolignan X (
3) exhibited cytotoxicity against Hep2, WiDr, Daoy, and MCF-7 cell lines with ED
50 values of 3.60, 4.45, 6.07, and 13.7 μg/mL, respectively. Turformosin A (
1), (−)-(7′
S,8′
S)-
threo-carolignan X (
3), methoxyhydroquinone-4-β-d-glucopyranoside (
5), and methoxy-hydroquinone-1-β-d-glucopyranoside (
6), exhibited similar anti-oxidative activity. Hep2 cells treated with 10 μg/mL of
3 showed elevation of sub-G1 population (from 20% at 8 h to 60% at 48 h), and activation of caspase-9/caspase-3/PARP cascade. Compound
3 induced intrinsic apoptotic pathway in Hep2 cells with dose and time dependence (10 μg/mL for 8 h).
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