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Keywords = triantennary galactose ligand

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13 pages, 907 KiB  
Article
Synthesis and Excellent Duplex Stability of Oligonucleotides Containing 2′-Amino-LNA Functionalized with Galactose Units
by Rajesh Kumar, Annika Ries and Jesper Wengel
Molecules 2017, 22(5), 852; https://doi.org/10.3390/molecules22050852 - 21 May 2017
Cited by 8 | Viewed by 7641
Abstract
A convenient method for the preparation of oligonucleotides containing internally-attached galactose and triantennary galactose units has been developed based on click chemistry between 2′-N-alkyne 2′-amino-LNA nucleosides and azido-functionalized galactosyl building blocks. The synthesized oligonucleotides show excellent binding affinity and selectivity towards [...] Read more.
A convenient method for the preparation of oligonucleotides containing internally-attached galactose and triantennary galactose units has been developed based on click chemistry between 2′-N-alkyne 2′-amino-LNA nucleosides and azido-functionalized galactosyl building blocks. The synthesized oligonucleotides show excellent binding affinity and selectivity towards complementary DNA/RNA strands with an increase in the melting temperature of up to +23.5 °C for triply-modified variants. Full article
(This article belongs to the Special Issue Synthesis and Applications of Oligonucleotide Conjugates)
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