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Keywords = sparganii rhizoma

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16 pages, 989 KiB  
Article
Chemical Composition, Antioxidative and Anticancer Activities of the Essential Oil: Curcumae RhizomaSparganii Rhizoma, a Traditional Herb Pair
by Guan-Ling Xu, Di Geng, Meng Xie, Kai-Yue Teng, Yu-Xin Tian, Zi-Zhen Liu, Cheng Yan, Yan Wang, Xia Zhang, Yan Song, Yue Yang and Gai-Mei She
Molecules 2015, 20(9), 15781-15796; https://doi.org/10.3390/molecules200915781 - 28 Aug 2015
Cited by 43 | Viewed by 8656
Abstract
As a classical herb pair in clinics of traditional Chinese medicine, Curcumae RhizomaSparganii Rhizoma (HP CR–SR) is used for activating blood circulation to remove blood stasis. The essential components in HP CR–SR and its single herbs were comparatively analyzed using gas [...] Read more.
As a classical herb pair in clinics of traditional Chinese medicine, Curcumae RhizomaSparganii Rhizoma (HP CR–SR) is used for activating blood circulation to remove blood stasis. The essential components in HP CR–SR and its single herbs were comparatively analyzed using gas chromatography-mass spectrometry data. 66, 22, and 54 components in volatile oils of Curcumae Rhizoma, Sparganii Rhizoma, and HP CR–SR were identified, and total contents accounted for 75.416%, 91.857%, and 79.553% respectively. The thirty-eight components were found in HP CR–SR, and not detected in single herbs Curcumae Rhizoma and Sparganii Rhizoma. The highest radical trapping action was seen by an essential oil of HP CR–SR (IC50 = 0.59 ± 0.04 mg/mL). Furthermore, the HP CR–SR essential oil showed more remarkable cytotoxicity on tumor cell lines than that of the single herbs Curcumae Rhizoma and Sparganii Rhizoma in a dose-dependent manner: IC50 values showing 32.32 ± 5.31 μg/mL (HeLa), 34.76 ± 1.82 μg/mL (BGC823), 74.84 ± 1.66 μg/mL (MCF-7), 66.12 ± 11.23 μg/mL (SKOV3), and 708.24 ± 943.91 μg/mL (A549), respectively. In summary, the essential oil of HP CR–SR is different from any one of Curcumae Rhizoma and Sparganii Rhizoma, nor simply their superposition, and HP CR–SR oil presented more remarkable anticancer and antioxidant activities compared with Curcumae Rhizoma and Sparganii Rhizoma oils. Full article
(This article belongs to the Collection Herbal Medicine Research)
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15 pages, 394 KiB  
Article
Response Surface Optimized Ultrasonic-Assisted Extraction of Flavonoids from Sparganii Rhizoma and Evaluation of Their in Vitro Antioxidant Activities
by Xinsheng Wang, Qinan Wu, Yanfang Wu, Guangyun Chen, Wei Yue and Qiaoli Liang
Molecules 2012, 17(6), 6769-6783; https://doi.org/10.3390/molecules17066769 - 4 Jun 2012
Cited by 66 | Viewed by 7216
Abstract
An efficient ultrasound-assisted extraction technique was employed to extract total flavonoids from Sparganii rhizoma. The optimum extraction conditions for the highest yield of total flavonoids were ethanol concentration 53.62%, ultrasonication time 29.41 min and ultrasound power 300 W, which were determined using response [...] Read more.
An efficient ultrasound-assisted extraction technique was employed to extract total flavonoids from Sparganii rhizoma. The optimum extraction conditions for the highest yield of total flavonoids were ethanol concentration 53.62%, ultrasonication time 29.41 min and ultrasound power 300 W, which were determined using response surface methodology. The extraction yields of the optimal ultrasound-assisted extraction were higher than using conventional extraction. The crude extract was then purified on a polyamide resin, whereby the flavonoids content in the purified extract increased to 94.62%. The antioxidant activities of the purified flavonoids including DPPH radical scavenging activity, ABTS+ radical scavenging activity, reducing power, hydroxyl radical scavenging activity and superoxide anion scavenging activity, were evaluated in vitro, which suggested that the flavonoids showed significant antioxidant activities. Rutin, kaempferol and formononetin were identified in the extract by comparing relative retention times and UV-Vis spectra with those of reference standards. Full article
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