Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

Article Types

Countries / Regions

Search Results (1)

Search Parameters:
Keywords = pretreatment-free cell isolation protocol

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
16 pages, 3010 KB  
Article
A Protocol for the Isolation of Oval Cells without Preconditioning
by Rocío Olivera-Salazar, Aránzazu Sánchez, Blanca Herrera, Juan García-Sáez, Luz Vega-Clemente, Pedro Villarejo Campos, Damián García-Olmo and Mariano García-Arranz
Int. J. Mol. Sci. 2024, 25(19), 10497; https://doi.org/10.3390/ijms251910497 - 29 Sep 2024
Cited by 1 | Viewed by 1462
Abstract
Oval cells (OCs) is the name of hepatic progenitor cells (HPCs) in rodents. They are a small population of cells in the liver with the remarkable ability to proliferate and regenerate hepatocytes and cholangiocytes in response to acute liver damage. Isolating OCs generally [...] Read more.
Oval cells (OCs) is the name of hepatic progenitor cells (HPCs) in rodents. They are a small population of cells in the liver with the remarkable ability to proliferate and regenerate hepatocytes and cholangiocytes in response to acute liver damage. Isolating OCs generally requires a pretreatment with special diets, chemicals, and/or surgery to induce hepatic damage and OC proliferation in mice. Unfortunately, these pretreatments are not only painful for the mice but also increase the cost of the assays, and the effects on the different organs as well as on various liver cells are still unclear. Therefore, the search for a protocol to obtain OCs without prior liver damage is mandatory. In our study, we present a protocol to isolate murine OCs from healthy liver (HL-OCs) and compare them with OCs isolated from mice pretreated with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC-OCs). Our results demonstrated that cells derived from untreated mice exhibited similar behavior to those from treated mice in terms of surface marker expression, proliferation, and differentiation capacity. Therefore, given the impracticability of isolating human cells with prior hepatotoxic treatment, our model holds promise for enabling the isolation of progenitor cells from human tissue in the future. This advancement could prove invaluable for translational medicine in the understanding and treatment of liver diseases. Full article
(This article belongs to the Special Issue Chronic Liver Disease and Hepatocellular Carcinoma)
Show Figures

Figure 1

Back to TopTop