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Potato stem removal is one of the critical technical problems of potato mechanized harvesting; it directly affects the quality of potato harvesting and potato storage. There have been several studies on potato stem removal mechanisms. In practice, however, it was found that the potato stem removal rate was greatly influenced by the posture of the stem before it entered the removal mechanism. In this study, we designed a potato stem posture adjustment mechanism consisting of elastic curtains. A test rig was built to investigate the effect of curtain height, curtain width, and curtain suspension height on potato passage rate and potato stem removal rate. The Box–Behnken design (BBD), combined with the response surface method, was used to conduct the test. The optimal construction and installation parameters for each elastic curtain were determined as 278.93 mm for the curtain height, 20 mm for the curtain width, and 260 mm for the curtain suspension height. The predicted values of potato passage rate and potato stem removal rate under the optimal parameters were 92.36% and 82.83%, which were consistent with the validation test results. Based on the optimization results, a rigid-flexible coupled simulation model for a potato stem transported-posture adjustment process based on Abaqus and Adams was constructed. The maximum impact of the elastic curtain of the stem posture adjustment mechanism on the potato stem was 15.91 N and caused the stem to spring back. The projection angle β′ of the stem posture angle in the xoz plane before posture adjustment was 19.07°, and the β′ of the stem after posture adjustment was 87.18°. At this time, the stem was basically parallel to the rod of the separating sieve and had a high probability of falling from the gap of the bar to complete the removal of the stem. Overall, the stem transport position adjustment mechanism effectively adjusted the stem transported posture and improved the stem removal rate in potato mechanical harvesting. Full article