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Keywords = plastic media blasting

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12 pages, 1684 KiB  
Article
Emerging Issues on Antibiotic-Resistant Bacteria Colonizing Plastic Waste in Aquatic Ecosystems
by Ifra Ferheen, Roberto Spurio and Stefania Marcheggiani
Antibiotics 2024, 13(4), 339; https://doi.org/10.3390/antibiotics13040339 - 8 Apr 2024
Cited by 5 | Viewed by 2741
Abstract
Antibiotic-resistant bacteria (ARB) adhesion onto plastic substrates is a potential threat to environmental and human health. This current research investigates the prevalence of two relevant human pathogens, Staphylococcus spp. and Klebsiella spp., and their sophisticated equipment of antibiotic-resistant genes (ARGs), retrieved from plastic [...] Read more.
Antibiotic-resistant bacteria (ARB) adhesion onto plastic substrates is a potential threat to environmental and human health. This current research investigates the prevalence of two relevant human pathogens, Staphylococcus spp. and Klebsiella spp., and their sophisticated equipment of antibiotic-resistant genes (ARGs), retrieved from plastic substrates submerged into an inland water body. The results of microbiological analysis on selective and chromogenic media revealed the presence of colonies with distinctive phenotypes, which were identified using biochemical and molecular methods. 16S rDNA sequencing and BLAST analysis confirmed the presence of Klebsiella spp., while in the case of Staphylococcus spp., 63.6% of strains were found to be members of Lysinibacillus spp., and the remaining 36.3% were identified as Exiguobacterium acetylicum. The Kirby–Bauer disc diffusion assay was performed to test the susceptibility of the isolates to nine commercially available antibiotics, while the genotypic resistant profile was determined for two genes of class 1 integrons and eighteen ARGs belonging to different classes of antibiotics. All isolated bacteria displayed a high prevalence of resistance against all tested antibiotics. These findings provide insights into the emerging risks linked to colonization by potential human opportunistic pathogens on plastic waste commonly found in aquatic ecosystems. Full article
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18 pages, 7733 KiB  
Article
On the Effectiveness of Different Surface Finishing Techniques on A357.0 Parts Produced by Laser-Based Powder Bed Fusion: Surface Roughness and Fatigue Strength
by Lucia Denti and Antonella Sola
Metals 2019, 9(12), 1284; https://doi.org/10.3390/met9121284 - 29 Nov 2019
Cited by 27 | Viewed by 5634
Abstract
Laser-based powder bed fusion (L-PBF) is an additive manufacturing (AM) technique that uses a computer-controlled laser beam as the energy source to consolidate a metal powder according to a layer-upon-layer strategy in order to manufacture a three dimensional part. This opens the way [...] Read more.
Laser-based powder bed fusion (L-PBF) is an additive manufacturing (AM) technique that uses a computer-controlled laser beam as the energy source to consolidate a metal powder according to a layer-upon-layer strategy in order to manufacture a three dimensional part. This opens the way for an unprecedented freedom in geometry, but the layer-wise build-up strategy typically results in a very poor surface finish, which is affected by the staircase effect and by the presence of partially molten particles. Surface finishing treatments are therefore necessary to obtain an adequate surface finish, to improve the fatigue behavior and to meet mechanical and aesthetic needs. The present contribution systematically compares numerous surface finishing techniques, including laser shock processing, plastic media blasting, sand blasting, ceramic shot peening and metal shot peening with steel particles of different sizes (ϕ = 0.2 mm and ϕ = 0.4 mm). The results show that all the proposed methods improve the surface quality and the fatigue life of A357.0 L-PBF parts. However, the achievement of the lowest surface roughness does not necessarily correspond to the best fatigue performance, thus suggesting that multiple mechanisms may be active and that besides surface roughness also residual stresses contribute to increase the fatigue strength. Full article
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