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Keywords = pMAG vectors

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10 pages, 1721 KiB  
Brief Report
pMAGs: A Versatile and Efficient Vector System for Multi-Gene Studies in Plants
by Mengyue Zhang, Jing Liu, Han Zhao, Zhaojun Ding, Xiaoxuan Li and Zipeng Yu
Plants 2025, 14(16), 2602; https://doi.org/10.3390/plants14162602 - 21 Aug 2025
Abstract
In molecular biology studies, suitable vectors are fundamental tools; however, most vectors can only express one target gene, which limits the ability to study multiple genes simultaneously within the same plant tissue. The traditional method for achieving multi-gene co-expression involves co-transferring multiple plasmids [...] Read more.
In molecular biology studies, suitable vectors are fundamental tools; however, most vectors can only express one target gene, which limits the ability to study multiple genes simultaneously within the same plant tissue. The traditional method for achieving multi-gene co-expression involves co-transferring multiple plasmids into plant tissues, but this approach is often inefficient due to the difficulty of successfully transforming multiple plasmids at once. To overcome this limitation, we have developed a series of vectors, called pMAGs (Multigene Assembly Genetic vectors), capable of simultaneously expressing or silencing two or three different genes in plants. These vectors not only provide an optimal solution for a wide range of biological experiments but also work effectively across numerous plant species. Full article
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