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Keywords = murine PYHIN proteins

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15 pages, 4162 KiB  
Article
A Variety of Mouse PYHIN Proteins Restrict Murine and Human Retroviruses
by Sümeyye Erdemci-Evin, Matteo Bosso, Veronika Krchlikova, Wibke Bayer, Kerstin Regensburger, Martha Mayer, Ulf Dittmer, Daniel Sauter, Dorota Kmiec and Frank Kirchhoff
Viruses 2024, 16(4), 493; https://doi.org/10.3390/v16040493 - 23 Mar 2024
Cited by 1 | Viewed by 1890
Abstract
PYHIN proteins are only found in mammals and play key roles in the defense against bacterial and viral pathogens. The corresponding gene locus shows variable deletion and expansion ranging from 0 genes in bats, over 1 in cows, and 4 in humans to [...] Read more.
PYHIN proteins are only found in mammals and play key roles in the defense against bacterial and viral pathogens. The corresponding gene locus shows variable deletion and expansion ranging from 0 genes in bats, over 1 in cows, and 4 in humans to a maximum of 13 in mice. While initially thought to act as cytosolic immune sensors that recognize foreign DNA, increasing evidence suggests that PYHIN proteins also inhibit viral pathogens by more direct mechanisms. Here, we examined the ability of all 13 murine PYHIN proteins to inhibit HIV-1 and murine leukemia virus (MLV). We show that overexpression of p203, p204, p205, p208, p209, p210, p211, and p212 strongly inhibits production of infectious HIV-1; p202, p207, and p213 had no significant effects, while p206 and p214 showed intermediate phenotypes. The inhibitory effects on infectious HIV-1 production correlated significantly with the suppression of reporter gene expression by a proviral Moloney MLV-eGFP construct and HIV-1 and Friend MLV LTR luciferase reporter constructs. Altogether, our data show that the antiretroviral activity of PYHIN proteins is conserved between men and mice and further support the key role of nuclear PYHIN proteins in innate antiviral immunity. Full article
(This article belongs to the Special Issue Innate Sensing and Restriction of Retroviruses)
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