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Keywords = mid-density genotyping assay

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18 pages, 3958 KiB  
Article
Discovery of Major Quantitative Trait Loci and Candidate Genes for Fresh Seed Dormancy in Groundnut
by Deekshitha Bomireddy, Sunil S. Gangurde, Murali T. Variath, Pasupuleti Janila, Surendra S. Manohar, Vinay Sharma, Sejal Parmar, Dnyaneshwar Deshmukh, Mangala Reddisekhar, Devarapalli Mohan Reddy, Palagiri Sudhakar, Bommu Veera Bhaskara Reddy, Rajeev K. Varshney, Baozhu Guo and Manish K. Pandey
Agronomy 2022, 12(2), 404; https://doi.org/10.3390/agronomy12020404 - 6 Feb 2022
Cited by 14 | Viewed by 3685
Abstract
Spanish bunch groundnut varieties occupy most of the cultivated area in Asia and Africa, and these varieties lack required 2-3 weeks of fresh seed dormancy (FSD) hampering kernel quality. Genomic breeding can help to improve commercial groundnut cultivars for FSD in a shorter [...] Read more.
Spanish bunch groundnut varieties occupy most of the cultivated area in Asia and Africa, and these varieties lack required 2-3 weeks of fresh seed dormancy (FSD) hampering kernel quality. Genomic breeding can help to improve commercial groundnut cultivars for FSD in a shorter time with greater precision. In this regard, a recombinant inbred line (RIL) population from the cross ICGV 02266 (non-dormant) × ICGV 97045 (dormant) was developed and genotyped with a 5 K mid-density genotyping assay. A linkage map was constructed with 325 SNP loci spanning a total map length of 2335.3 cM and five major QTLs were identified on chromosomes Ah01, Ah11, Ah06, Ah16 and Ah17. Based on differential gene expression using transcriptomic information from dormant (Tifrunner) and non-dormant (ICGV 91114) genotypes, histone deacetylases, histone-lysine N-methyltransferase, cytochrome P450, protein kinases, and ethylene-responsive transcription factor were identified as key regulators involved in the hormonal regulation of dormancy. Six Kompetitive Allele Specific PCR (KASP) markers were successfully validated in the diverse panel including selected RILs of the same population and germplasm lines. These validated KASP markers could facilitate faster breeding of new varieties with desired dormancy using marker-assisted early generation selection. Full article
(This article belongs to the Topic Plant Breeding, Genetics and Genomics)
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