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Keywords = metanesulfonic acid

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17 pages, 2772 KiB  
Article
On the Optimization of Carob Seed Peel Extraction Using Aqueous-Based Acidic Systems
by Bruno Medronho, Oumaima Boutoub, Hugo Duarte, Maria José Aliaño-González, Rui Guerra, António Brázio, Sandra Gonçalves and Anabela Romano
Molecules 2025, 30(7), 1397; https://doi.org/10.3390/molecules30071397 - 21 Mar 2025
Viewed by 644
Abstract
Carob fruit utilization remains limited, with most of their commercial value derived from locust bean gum, which is obtained from seed endosperm. Efficient extraction requires dehusking, which is traditionally performed under harsh conditions. This study aims to develop and optimize a milder, more [...] Read more.
Carob fruit utilization remains limited, with most of their commercial value derived from locust bean gum, which is obtained from seed endosperm. Efficient extraction requires dehusking, which is traditionally performed under harsh conditions. This study aims to develop and optimize a milder, more sustainable dehusking method while preserving seed quality for industrial applications. Various aqueous-based solvents were tested, leading to the selection of metanesulfonic acid (CH4O3S). A Box–Behnken design with response surface methodology optimized the process, using husk removal efficiency as the response variable. The optimized conditions were 24.5 g of seeds treated in 50 mL of a solvent mixture (41% CH4O3S and 59% H2SO4) at 90 °C for 10 min, followed by washing by 5 min with water (87 mL). The treated seeds were analyzed using colorimetry assays and diffusive reflectance spectroscopy and benchmarked against both pristine and dehusked seeds from a local company. The resulting seeds remained morphologically intact and exhibited appealing color characteristics comparable to commercial samples. The optimized method ensured intact seed morphology and color characteristics comparable to commercial standards, offering a viable alternative to conventional H2SO4-based dehusking. Furthermore, this study also highlights for the first time the effectiveness of diffusive reflectance spectroscopy as a rapid and straightforward tool for assessing the dehusking process. Full article
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10 pages, 41584 KiB  
Article
Efficacy of a Topical Wound Agent Methanesulfonic Acid and Dimethylsulfoxide on In Vitro Biofilms
by Saskia Schwarzer, Michael Radzieta, Slade O. Jensen and Matthew Malone
Int. J. Mol. Sci. 2021, 22(17), 9471; https://doi.org/10.3390/ijms22179471 - 31 Aug 2021
Cited by 10 | Viewed by 3577
Abstract
A topical desiccating wound agent containing methanesulfonic acid, dimethylsulfoxide and amorphous silica was evaluated in three in vitro models for its efficacy against biofilms produced by Pseudomonas aeruginosa (ATCC-15442) and Staphylococcus aureus (ATCC-6538). The in vitro biofilm models used were; the MBEC Assay [...] Read more.
A topical desiccating wound agent containing methanesulfonic acid, dimethylsulfoxide and amorphous silica was evaluated in three in vitro models for its efficacy against biofilms produced by Pseudomonas aeruginosa (ATCC-15442) and Staphylococcus aureus (ATCC-6538). The in vitro biofilm models used were; the MBEC Assay®, Centre for Disease Control (CDC) Biofilm Reactor® and a Semi-solid biofilm model. A 30-s exposure of a topical wound desiccating agent was used in each model. A complete eradication of viable cells was demonstrated in all models for both strains (p < 0.0001). Imaging with scanning electron microscopy (SEM) was performed where possible. All three models demonstrated complete eradication of viable cells with a 30 s application of a topical wound desiccating agent. Full article
(This article belongs to the Special Issue Novel Biomaterials for Wound Healing)
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