This study focused on the interactions of pea (
Pisum sativum L.) plants with phytopathogenic and beneficial fungi. Here, we examined whether the lysin-motif (LysM) receptor-like kinase
PsLYK9 is directly involved in the perception of long- and short-chain chitooligosaccharides (COs) released after
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This study focused on the interactions of pea (
Pisum sativum L.) plants with phytopathogenic and beneficial fungi. Here, we examined whether the lysin-motif (LysM) receptor-like kinase
PsLYK9 is directly involved in the perception of long- and short-chain chitooligosaccharides (COs) released after hydrolysis of the cell walls of phytopathogenic fungi and identified in arbuscular mycorrhizal (AM) fungal exudates. The identification and analysis of pea mutants impaired in the
lyk9 gene confirmed the involvement of
PsLYK9 in symbiosis development with AM fungi. Additionally,
PsLYK9 regulated the immune response and resistance to phytopathogenic fungi, suggesting its bifunctional role. The existence of co-receptors may provide explanations for the potential dual role of
PsLYK9 in the regulation of interactions with pathogenic and AM fungi. Co-immunoprecipitation assay revealed that
PsLYK9 and two proposed co-receptors,
PsLYR4 and
PsLYR3, can form complexes. Analysis of binding capacity showed that
PsLYK9 and
PsLYR4, synthesized as extracellular domains in insect cells, were able to bind the deacetylated (DA) oligomers CO5-DA–CO8-DA. Our results suggest that the receptor complex consisting of
PsLYK9 and
PsLYR4 can trigger a signal pathway that stimulates the immune response in peas. However,
PsLYR3 seems not to be involved in the perception of CO4-5, as a possible co-receptor of
PsLYK9.
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