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Keywords = joint-mimicking organoid

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14 pages, 5301 KiB  
Article
Articular Tissue-Mimicking Organoids Derived from Mesenchymal Stem Cells and Induced Pluripotent Stem Cells
by Zhong Alan Li, Jiangyinzi Shang, Shiqi Xiang, Eileen N. Li, Haruyo Yagi, Kanyakorn Riewruja, Hang Lin and Rocky S. Tuan
Organoids 2022, 1(2), 135-148; https://doi.org/10.3390/organoids1020011 - 14 Nov 2022
Cited by 23 | Viewed by 5058
Abstract
Organoids offer a promising strategy for articular tissue regeneration, joint disease modeling, and development of precision medicine. In this study, two types of human stem cells—primary mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs)—were employed to engineer organoids that mimicked bone, [...] Read more.
Organoids offer a promising strategy for articular tissue regeneration, joint disease modeling, and development of precision medicine. In this study, two types of human stem cells—primary mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs)—were employed to engineer organoids that mimicked bone, cartilage and adipose tissue, three key tissue components in articular joints. Prior to organoidogenesis, the iPSCs were first induced into mesenchymal progenitor cells (iMPCs). After characterizing the MSCs and iMPCs, they were used to generate cell-embedded extracellular matrix (ECM) constructs, which then underwent self-aggregation and lineage-specific differentiation in different induction media. Hydroxyapatite nanorods, an osteoinductive bioceramic, were leveraged to generate bone and osteochondral organoids, which effectively enhanced mineralization. The phenotypes of the generated organoids were confirmed on the basis of gene expression profiling and histology. Our findings demonstrate the feasibility and potential of generating articular tissue-recapitulating organoids from MSCs and iPSCs. Full article
(This article belongs to the Special Issue Organoids Mimicking Articular Tissue)
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