Search Results (2)

Fluorescence in situ hybridization (FISH) is an indispensable technique for studying chromosomes in plants. However, traditional FISH methods, such as BAC, rDNA, tandem repeats, and distributed repetitive sequence probe-based FISH, have certain limitations, including difficulties in probe synthesis, low sensitivity, cross-hybridization, and limited resolution. In contrast, oligo-based FISH represents a more efficient method for chromosomal studies in plants. Oligo probes are computationally designed and synthesized for any plant species with a sequenced genome and are suitable for single and repetitive DNA sequences, entire chromosomes, or chromosomal segments. Furthermore, oligo probes used in the FISH experiment provide high specificity, resolution, and multiplexing. Moreover, oligo probes made from one species are applicable for studying other genetically and taxonomically related species whose genome has not been sequenced yet, facilitating molecular cytogenetic studies of non-model plants. However, there are some limitations of oligo probes that should be considered, such as requiring prior knowledge of the probe design process and FISH signal issues with shorter probes of background noises during oligo-FISH experiments. This review comprehensively discusses de novo oligo probe synthesis with more focus on single-copy DNA sequences, preparation, improvement, and factors that affect oligo-FISH efficiency. Furthermore, this review highlights recent applications of oligo-FISH in a wide range of plant chromosomal studies. Full article

An on-chip 64-channel hybrid (de)multiplexer for wavelength-division multiplexing (WDM) and mode-division multiplexing (MDM) is designed and demonstrated on a 220 nm SOI platform for the demands of large capacity optical interconnections. The designed hybrid (de)multiplexer includes a 4-channel mode (de)multiplexer and 16-channel wavelength-division (de)multiplexers. The mode (de)multiplexer is comprised of cascaded asymmetric directional couplers supporting coupling between fundamental TE mode and higher-order modes with low crosstalks in a wide wavelength range. The wavelength-division (de)multiplexers consist of two bi-directional micro-ring resonator arrays for four 16-channel WDM signals. Micro-heaters are placed on the micro-resonators for thermal tuning. According to the experimental results, the excess loss is <3.9 dB in one free spectral range from 1522 nm to 1552 nm and <5.6 dB in three free spectral ranges from 1493 nm to 1583 nm. The intermode crosstalks are −23.2 dB to −33.2 dB, and the isolations between adjacent and nonadjacent wavelength channels are about −17.1 dB and −22.3 dB, respectively. The thermal tuning efficiency is ∼2.22 mW/nm over one free spectral range. Full article