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Keywords = gigaporous microspheres

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12 pages, 5089 KB  
Article
The Construction of an Aqueous Two-Phase System to Solve Weak-Aggregation of Gigaporous Poly(Styrene-Divinyl Benzene) Microspheres
by Donglai Zhang, Weiqing Zhou, Juan Li, Yace Mi, Zhiguo Su and Guanghui Ma
Polymers 2016, 8(5), 142; https://doi.org/10.3390/polym8050142 - 26 Apr 2016
Cited by 8 | Viewed by 6653
Abstract
Gigaporous poly(styrene-divinyl benzene) microspheres made via the surfactant reverse micelles swelling method had a controllable pore size of 100–500 nm. These microspheres had unique advantages in biomacromolecule separation and enzymes immobilization. However, the obtained microspheres adhered to each other in the preparation process. [...] Read more.
Gigaporous poly(styrene-divinyl benzene) microspheres made via the surfactant reverse micelles swelling method had a controllable pore size of 100–500 nm. These microspheres had unique advantages in biomacromolecule separation and enzymes immobilization. However, the obtained microspheres adhered to each other in the preparation process. Though the weak aggregation could be re-dispersed easily by mechanical force, it will be difficult to scale up. By analyzing the formation mechanism of the aggregates, a method was presented to rebuild the interface between the internal aqueous channel and the external continuous phase by constructing an aqueous two-phase system (ATPS). Based on the ATPS, the method of emulsification, stirring speed, and surfactant concentration in oil phase were optimized. Under the optimum condition (screen emulsification method, 120 rpm for polymerization and 55% surfactant), the microspheres with a controllable particle size of 10–40 μm and a pore size of about 150 nm were obtained. This new method could significantly decrease the weak-aggregation of microspheres. Full article
(This article belongs to the Special Issue Selected Papers from ASEPFPM2015)
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10 pages, 5445 KB  
Article
Towards A Deeper Understanding of the Interfacial Adsorption of Enzyme Molecules in Gigaporous Polymeric Microspheres
by Weichen Wang, Weiqing Zhou, Wei Wei, Juan Li, Dongxia Hao, Zhiguo Su and Guanghui Ma
Polymers 2016, 8(4), 116; https://doi.org/10.3390/polym8040116 - 7 Apr 2016
Cited by 4 | Viewed by 6387
Abstract
Compared with the one immobilized in the conventional mesoporous microspheres, the enzyme immobilized in gigaporous microspheres showed much higher activity and better stability. To gain a deeper understanding, we herein selected lipase as a prototype to comparatively analyze the adsorption behavior of lipase [...] Read more.
Compared with the one immobilized in the conventional mesoporous microspheres, the enzyme immobilized in gigaporous microspheres showed much higher activity and better stability. To gain a deeper understanding, we herein selected lipase as a prototype to comparatively analyze the adsorption behavior of lipase at interfaces in gigaporous and mesoporous polystyrene microspheres at very low lipase concentration, and further compared with the adsorption on a completely flat surface (a chip). Owing to the limited space of narrow pores, lipase molecules were inclined to be adsorbed as a monolayer in mesoporous microspheres. During this process, the interaction between lipase molecules and the interface was stronger, which could result in the structural change of lipase molecular and compromised specific activity. In addition to monolayer adsorption, more multilayer adsorption of enzyme molecules also occurred in gigaporous microspheres. Besides the adsorption state, the pore curvature also affected the lipase adsorption. Due to the multilayer adsorption, the excellent mass transfer properties for the substrate and the product in the large pores, and the small pore curvature, lipase immobilized in gigaporous microspheres showed better behaviors. Full article
(This article belongs to the Special Issue Selected Papers from ASEPFPM2015)
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