Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline

Journals

Article Types

Countries / Regions

Search Results (1)

Search Parameters:
Keywords = crizotinb

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
10 pages, 1327 KB  
Communication
A Triple Staining Method for Accurate Cell Cycle Analysis Using Multiparameter Flow Cytometry
by Lin Qiu, Ming Liu and Konglun Pan
Molecules 2013, 18(12), 15412-15421; https://doi.org/10.3390/molecules181215412 - 11 Dec 2013
Cited by 16 | Viewed by 18116
Abstract
Cell cycle analysis is important for cancer research. We present herein a novel method for accurate cell cycle analysis. This method analyzes the cell cycle by multiparameter flow cytometry based on simultaneously labeling the cell nuclear DNA, RNA, and phosphorylated mitotic nuclei protein, [...] Read more.
Cell cycle analysis is important for cancer research. We present herein a novel method for accurate cell cycle analysis. This method analyzes the cell cycle by multiparameter flow cytometry based on simultaneously labeling the cell nuclear DNA, RNA, and phosphorylated mitotic nuclei protein, using Hoechst 33342, pyronin Y, and MPM-2-Cy5, respectively, and our results demonstrated that this method could effectively divide the cell cycle into G0, G1, S, G2, and M phases. We further tested this method using the clinical anticancer agents crizotinib and taxol, and the results clearly illustrated that crizotinib and taxol arrested Jurkat cells in G0 and M phase, respectively. These results indicate that this method could be a very useful tool for cytokinetic and pharmacological research. Full article
Show Figures

Figure 1

Back to TopTop