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Keywords = a novel BMDCs culturing with hydrogel substrate (CCHS)

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19 pages, 8623 KiB  
Article
Photocurable Hydrogel Substrate—Better Potential Substitute on Bone-Marrow-Derived Dendritic Cells Culturing
by Jiewen Deng, Yao Xie, Jian Shen, Qing Gao, Jing He, Hong Ma, Yongli Ji, Yong He and Meixiang Xiang
Materials 2022, 15(9), 3322; https://doi.org/10.3390/ma15093322 - 5 May 2022
Cited by 7 | Viewed by 3210
Abstract
Dendritic cells (DCs) are recognized as the most effective antigen-presenting cells at present. DCs have corresponding therapeutic effects in tumor immunity, transplantation immunity, infection inflammation and cardiovascular diseases, and the activation of T cells is dependent on DCs. However, normal bone-marrow-derived Dendritic cells [...] Read more.
Dendritic cells (DCs) are recognized as the most effective antigen-presenting cells at present. DCs have corresponding therapeutic effects in tumor immunity, transplantation immunity, infection inflammation and cardiovascular diseases, and the activation of T cells is dependent on DCs. However, normal bone-marrow-derived Dendritic cells (BMDCs) cultured on conventional culture plates are easy to be activated during culturing, and it is difficult to imitate the internal immune function. Here, we reported a novel BMDCs culturing with hydrogel substrate (CCHS), where we synthesized low substituted Gelatin Methacrylate-30 (GelMA-30) hydrogels and used them as a substitute for conventional culture plates in the culture and induction of BMDCs in vitro. The results showed that 5% GelMA-30 substrate was the best culture condition for BMDCs culturing. The low level of costimulatory molecules and the level of development-related transcription factors of BMDCs by CCHS were closer to that of spleen DCs and were capable of better promoting T cell activation and exerting an immune effect. CCHS was helpful to study the transformation of DCs from initial state to activated state, which contributes to the development of DC-T cell immunotherapy. Full article
(This article belongs to the Topic Advances and Applications of 2D Materials)
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