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Keywords = UHPLC-DAD-QTOF-MSMS

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16 pages, 2260 KiB  
Article
Purification and Identification of Flavonoids from Prunus mume and Its Anti-Photoaging Activities on UVB-Induced Human Keratinocytes
by Yuxuan Liu, Mengdie He, Xinxin Wei, Qifeng Han, Xiang Yang, Gangyi Huang, Chen Zhang, Mengqi Wu and Wenqing Zhang
Cosmetics 2025, 12(1), 19; https://doi.org/10.3390/cosmetics12010019 - 23 Jan 2025
Viewed by 1472
Abstract
Prunus mume (Armeniaca mume Sieb. var. mume f. viridicalyx (Makino) T. Y. Chen) is a common landscape plant. It has rich flavonoid content, which shows strong biological activities. In this study, the application potential of flavonoids in Prunus mume in anti-photoaging cosmetics [...] Read more.
Prunus mume (Armeniaca mume Sieb. var. mume f. viridicalyx (Makino) T. Y. Chen) is a common landscape plant. It has rich flavonoid content, which shows strong biological activities. In this study, the application potential of flavonoids in Prunus mume in anti-photoaging cosmetics was developed. Firstly, the flavonoids in Prunus mume were extracted by the heating reflux method, and the total flavonoid content reached 192 mg/g under the optimal process conditions determined by orthogonal testing. Then, the crude extract (PMCE) was purified by NKA-II resin column chromatography, and the purity of the flavonoids reached 83.99%. Subsequently, the Prunus mume purified product (PMPP) was analyzed by UHPLC-DAD-QTOF-MS/MS and 45 compounds were identified including flavonoid glycosides, phenolic acids and p-coumaroyl acetylated sucrose. In addition, in vitro biological experiments showed that PMPP had significant repair effects on UVB-induced photoaging of human keratinocytes (HaCaT), lowering reactive oxygen species (ROS) and malondialdehyde (MDA) levels, restoring superoxide dismutase (SOD) activity, and promoting hyaluronic acid (HA) secretion and Aquaporins-3 (AQP-3) levels. The results laid the foundation for the development of the flavonoids in Prunus mume as materials for anti-photoaging cosmetics. Full article
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16 pages, 3270 KiB  
Article
Effect of Different Post-Harvest Processing Methods on the Chemical Constituents of Notopterygium franchetii by an UHPLC-QTOF-MS-MS Metabolomics Approach
by Xueyan Su, Youjiao Wu, Ying Li, Yanfei Huang, Yuan Liu, Pei Luo and Zhifeng Zhang
Molecules 2019, 24(17), 3188; https://doi.org/10.3390/molecules24173188 - 2 Sep 2019
Cited by 25 | Viewed by 3591
Abstract
Notopterygium franchetii is a herb used in traditional Chinese medicine, where it is known as qianghuo. Its bioactive qualities are influenced by the post-harvest processing methods used (such as drying). However, changes in chemical components according to the drying method are unknown. [...] Read more.
Notopterygium franchetii is a herb used in traditional Chinese medicine, where it is known as qianghuo. Its bioactive qualities are influenced by the post-harvest processing methods used (such as drying). However, changes in chemical components according to the drying method are unknown. Fresh roots and rhizomes of N. franchetii were subjected to seven drying methods. Chromatography-mass spectrometry combined with targeted and untargeted analyses were used to investigate relationships between drying methods and chemical concentrations. According to targeted evaluations of the six main bioactive constituents, their total contents decreased significantly in all drying methods. Hierarchical clustering analysis of the drying methods and total metabolome detected 30 chemical constituents, for which heap maps were obtained. Hot air drying was the best processing method, producing the least chemical changes at the lowest cost, while shade drying caused the greatest chemical changes. In conclusion, the wide range of chemical changes in N. franchetii caused by drying was investigated. Such changes potentially affect the quality of herbal medicines. Full article
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