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Keywords = Sri Lankan algae

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13 pages, 2309 KiB  
Article
Fucoidan Purified from Sargassum polycystum Induces Apoptosis through Mitochondria-Mediated Pathway in HL-60 and MCF-7 Cells
by Ilekuttige Priyan Shanura Fernando, Kalu Kapuge Asanka Sanjeewa, Hyo Geun Lee, Hyun-Soo Kim, Andaravaas Patabadige Jude Prasanna Vaas, Hondamuni Ireshika Chathurani De Silva, Chandrika Malkanthi Nanayakkara, Dampegamage Thusitha Udayangani Abeytunga, Dae-Sung Lee, Jung-Suck Lee and You-Jin Jeon
Mar. Drugs 2020, 18(4), 196; https://doi.org/10.3390/md18040196 - 8 Apr 2020
Cited by 44 | Viewed by 5076
Abstract
Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum [...] Read more.
Fucoidans are biocompatible, heterogeneous, and fucose rich sulfated polysaccharides biosynthesized in brown algae, which are renowned for their broad-spectrum biofunctional properties. As a continuation of our preliminary screening studies, the present work was undertaken to extract polysaccharides from the edible brown algae Sargassum polycystum by a modified enzyme assisted extraction process using Celluclast, a food-grade cellulase, and to purify fucoidan by DEAE-cellulose anion exchange chromatography. The apoptotic and antiproliferative properties of the purified fucoidan (F5) were evaluated on HL-60 and MCF-7 cells. Structural features were characterized by FTIR and NMR analysis. F5 indicated profound antiproliferative effects on HL-60 leukemia and MCF-7 breast cancer cells with IC50 values of 84.63 ± 0.08 µg mL−1 and 93.62 ± 3.53 µg mL−1 respectively. Further, F5 treatment increased the apoptotic body formation, DNA damage, and accumulation of HL-60 and MCF-7 cells in the Sub-G1 phase of the cell cycle. The effects were found to proceed via the mitochondria-mediated apoptosis pathway. The Celluclast assisted extraction is a cost-efficient method of yielding fucoidan. With further studies in place, purified fucoidan of S. polycystum could be applied as functional ingredients in food and pharmaceuticals. Full article
(This article belongs to the Special Issue Fucoidans)
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