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Keywords = Sophora toromiro

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17 pages, 2961 KiB  
Article
Generation of Chloroplast Molecular Markers to Differentiate Sophora toromiro and Its Hybrids as a First Approach to Its Reintroduction in Rapa Nui (Easter Island)
by Ignacio Pezoa, Javier Villacreses, Miguel Rubilar, Carolina Pizarro, María Jesús Galleguillos, Troy Ejsmentewicz, Beatriz Fonseca, Jaime Espejo, Víctor Polanco and Carolina Sánchez
Plants 2021, 10(2), 342; https://doi.org/10.3390/plants10020342 - 10 Feb 2021
Cited by 7 | Viewed by 3627
Abstract
Sophora toromiro is an endemic tree of Rapa Nui with religious and cultural relevance that despite being extinct in the wild, still persists in botanical gardens and private collections around the world. The authenticity of some toromiro trees has been questioned because the [...] Read more.
Sophora toromiro is an endemic tree of Rapa Nui with religious and cultural relevance that despite being extinct in the wild, still persists in botanical gardens and private collections around the world. The authenticity of some toromiro trees has been questioned because the similarities among hybrid lines leads to misclassification of the species. The conservation program of toromiro has the objective of its reinsertion into Rapa Nui, but it requires the exact genotyping and certification of the selected plants in order to efficiently reintroduce the species. In this study, we present for the first time the complete chloroplast genome of S. toromiro and four other Sophora specimens, which were sequenced de-novo and assembled after mapping the raw reads to a chloroplast database. The length of the chloroplast genomes ranges from 154,239 to 154,473 bp. A total of 130–143 simple sequence repeats (SSR) loci and 577 single nucleotide polymorphisms (SNPs) were identified. Full article
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